Precision stereotaxic procedure for the mouse (Mus musculus): Method and instrumentation

Gb, Jones; Wahłsten, Douglas; Blom, Giorgio

doi:10.1016/0031-9384(77)90294-3

Cited by 11 publications

(3 citation statements)

References 4 publications

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“…Intravenous bolus injections in mice are commonly performed via the caudal vein; however, the blood‐brain barrier (BBB) limits access of some compounds to the central nervous system (CNS) using this injection site . Due to this and other limitations, many preclinical research studies require accurate injection of cells, drugs or contrast agent into precise locations in the brains of mice . Currently, these injections are performed using a stereotactic device with an atlas for reference, or a blind injection by hand with only external anatomical reference such as skull features.…”

Section: Introductionmentioning

confidence: 99%

“…9,10 Due to this and other limitations, many preclinical research studies require accurate injection of cells, drugs or contrast agent into precise locations in the brains of mice. [11][12][13] Currently, these injections are performed using a stereotactic device with an atlas for reference, or a blind injection by hand with only external anatomical reference such as skull features. Both of these techniques can result in inaccurate placement of needles due to human error and normal variations in anatomy, and may require multiple attempts for a successful insertion.…”

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confidence: 99%

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An ultra‐high field strength MR image‐guided robotic needle delivery system for in‐bore small animal interventions

2017

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Purpose: The purpose of this study was to develop and validate an image-guided robotic needle delivery system for accurate and repeatable needle targeting procedures in mouse brains inside the 12 cm inner diameter gradient coil insert of a 9.4 T MR scanner. Many preclinical research techniques require the use of accurate needle deliveries to soft tissues, including brain tissue. Soft tissues are optimally visualized in MR images, which offer high-soft tissue contrast, as well as a range of unique imaging techniques, including functional, spectroscopy and thermal imaging, however, there are currently no solutions for delivering needles to small animal brains inside the bore of an ultrahigh field MR scanner. This paper describes the mechatronic design, evaluation of MR compatibility, registration technique, mechanical calibration, the quantitative validation of the in-bore image-guided needle targeting accuracy and repeatability, and demonstrated the system's ability to deliver needles in situ. Methods: Our six degree-of-freedom, MR compatible, mechatronic system was designed to fit inside the bore of a 9.4 T MR scanner and is actuated using a combination of piezoelectric and hydraulic mechanisms. The MR compatibility and targeting accuracy of the needle delivery system are evaluated to ensure that the system is precisely calibrated to perform the needle targeting procedures. A semi-automated image registration is performed to link the robot coordinates to the MR coordinate system. Soft tissue targets can be accurately localized in MR images, followed by automatic alignment of the needle trajectory to the target. Intra-procedure visualization of the needle target location and the needle were confirmed through MR images after needle insertion. Results: The effects of geometric distortions and signal noise were found to be below threshold that would have an impact on the accuracy of the system. The system was found to have negligible effect on the MR image signal noise and geometric distortion. The system was mechanically calibrated and the mean image-guided needle targeting and needle trajectory accuracies were quantified in an image-guided tissue mimicking phantom experiment to be 178 AE 54 lm and 0.27 AE 0.65°, respectively. Conclusions: An MR image-guided system for in-bore needle deliveries to soft tissue targets in small animal models has been developed. The results of the needle targeting accuracy experiments in phantoms indicate that this system has the potential to deliver needles to the smallest soft tissue structures relevant in preclinical studies, at a wide variety of needle trajectories. Future work in the form of a fully-automated needle driver with precise depth control would benefit this system in terms of its applicability to a wider range of animal models and organ targets.

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Section: Introductionmentioning

confidence: 99%

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confidence: 99%

An ultra‐high field strength MR image‐guided robotic needle delivery system for in‐bore small animal interventions

2017

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“…The development of genetic engineering technology in the early 1990s has brought the mouse into a prominent role for investigations of the mammalian central nervous system. In these mouse-based neuroscience studies, stereotaxic surgical operations are essential for targeted delivery of chemicals, vectors, cells, or implantation of electrodes at specific locations in the brain (Athos and Storm, 2001; Cetin et al, 2006; Jones et al, 1977; Slotnick, 1972). Because the targeted delivery is essentially a blind operation, stereotaxic atlases are an essential resource for these surgeries.…”

Section: Introductionmentioning

confidence: 99%

AtlasGuide: Software for stereotaxic guidance using 3D CT/MRI hybrid atlases of developing mouse brains

Hsu

Jiang

et al. 2013

Journal of Neuroscience Methods

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Stereotaxic operations of the mouse brain are critically important for various types of neuroscience research studies, which include electrical recording of neural activities or site-targeted injection of stem cells, chemical tracers, and vectors, to name a few. To guide such operations, two-dimensional histology-based mouse brain atlases, such as the Paxinos and Franklin atlas, are widely used. Recently, computed tomography (CT) and magnetic resonance imaging (MRI) based hybrid three-dimensional (3D) atlases of developing mouse brains have been introduced. In this study, a new stereotaxic guidance software, called AtlasGuide, is introduced, which was developed to fully utilize the benefits of the 3D atlases for high-precision stereotaxic targeting. The AtlasGuide software provides functions to visualize oblique needle paths in 2D and 3D views, which allow investigators to simultaneously examine brain structures that could be damaged by the needle path and optimize the injection angles for high-precision trajectory selection through critical neural tissue. It allows reorientation and scaling of the atlases dynamically to match the orientation of the animal brain prepared for surgery, thereby eliminating the need to manually align the subject to the atlas, a procedure which is essential while using conventional 2D atlases. In addition, the software enables loading user-defined atlases when researchers need image-based guidance for different age groups, strains, or species. The software with integrated 3D stereotaxic mouse atlases is available for download at the http://lbam.med.jhmi.edu website.

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A self-adjusting head holder without ear-bars for guinea pigs

2018

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A self-adjusting head holder is designed to allow stable fixation and precise positioning (anterior-posterior, pitch, and roll) of guinea pig head in stereotaxic devices. These are achieved with no use of ear-bars. It is thus easy to use, preferable for studies of the auditory system, and for avoiding tissue damage of the ear in general. This head holder can accommodate various head sizes and is thus adapted for males and females of a large range of body weights, as confirmed for guinea pigs of 360-940 g. Moreover, this head holder is easy and cost-effective to manufacture, making it accessible for any lab. Here, we present background and mechanical rationale, the technical specifications, and step-by-step manufacturing instructions for the stainless-steel and the plastic MRI-compatible versions of our self-adjusting head holder.

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Precision stereotaxic procedure for the mouse (Mus musculus): Method and instrumentation

Cited by 11 publications

References 4 publications

An ultra‐high field strength MR image‐guided robotic needle delivery system for in‐bore small animal interventions

An ultra‐high field strength MR image‐guided robotic needle delivery system for in‐bore small animal interventions

AtlasGuide: Software for stereotaxic guidance using 3D CT/MRI hybrid atlases of developing mouse brains

A self-adjusting head holder without ear-bars for guinea pigs

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