Preclinical EIS Study of the Inflammatory Response Evolution of Pure Titanium Implant in Hank’s Biological Solution

Benea, Lidia; Bounegru, Iulian; Forray, Alexandra; Axente, Elena Roxana; Buruiana, Daniela Laura

doi:10.3390/molecules28124837

Cited by 9 publications

(3 citation statements)

References 32 publications

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“…It is believed that the release of titanium may be the result of an anodic corrosion process, where the TiO 2 layer is damaged. The cathodic part of the corrosion process involves oxygen reduction at physiological pH with the generation of ROS and non-radical compounds, such as hydrogen peroxide H 2 O 2 [ 5 , 23 , 24 ]. Lin et al [ 25 ] claim that the thickening of the TiO 2 layer in biological solutions is due to the continuous corrosion process of titanium implants in the human body.…”

Section: Discussionmentioning

confidence: 99%

Mitochondrial Redox Balance of Fibroblasts Exposed to Ti-6Al-4V Microplates Subjected to Different Types of Anodizing

Zalewska,

Antonowicz,

Szulimowska

et al. 2023

IJMS

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Despite the high biocompatibility of titanium and its alloys, the need to remove titanium implants is increasingly being debated due to the potential for adverse effects associated with long-term retention. Therefore, new solutions are being sought to enhance the biocompatibility of titanium implants. One of them is to increase the thickness of the passive layer of the implant made of titanium dioxide. We were the first to evaluate the effect of hard-anodized (type II) Ti-6Al-4V alloy discs on the cytotoxicity, mitochondrial function, and redox balance of fibroblasts mitochondria compared to standard-anodized (type III) and non-anodized discs. The study used fibroblasts obtained from human gingival tissue. The test discs were applied to the bottom of 12-well plates. Cells were cultured for 24 h and 7, 14, and 21 days and mitochondria were isolated. We demonstrated the occurrence of oxidative stress in the mitochondria of fibroblasts of all tested groups, regardless of the presence and type of anodization. Type II anodization prevented changes in complex II activity (vs. control). The lowest degree of citrate synthase inhibition occurred in mitochondria exposed to titanium discs with type II anodization. In the last phase of culture, the presence of type II anodization reduced the degree of cytochrome c oxidase inhibition compared to the other tests groups and the control group, and prevented apoptosis. Throughout the experiment, the release of titanium, aluminium, and vanadium ions from titanium discs with a hard-anodized passive layer was higher than from the other titanium discs, but decreased with time. The obtained results proved the existence of dysfunction and redox imbalance in the mitochondria of fibroblasts exposed to hard-anodized titanium discs, suggesting the need to search for new materials perhaps biodegradable in tissues of the human body.

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Section: Discussionmentioning

confidence: 99%

Mitochondrial Redox Balance of Fibroblasts Exposed to Ti-6Al-4V Microplates Subjected to Different Types of Anodizing

Zalewska,

Antonowicz,

Szulimowska

et al. 2023

IJMS

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“…У багатьох системах імплантатів використовують титановий сплав Ti-6Al-4V (клас 5) як основний матеріал для імплантату. Незважаючи на низку переваг титанових сплавів як матеріалів для імплантатів, вони чутливі до утворення біоплівки [12] і корозії [13]. Морфологія поверхні імплантату збільшує площу контакту з кісткою, що оточує, сприяючи остеоінтеграції та створюючи антизапальні умови для запобігання періімплантним захворюванням [14].…”

Section: обговоренняunclassified

Evaluation of anti-inflammatory properties on the surface of dental implants depending on the type of processing (Part 1)

Varzhapetian,

Shyshkin,

Strogonova

2024

Pathologia

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The aim. To study of the anti-inflammatory properties of the surface of commercial dental implants with different processing methods using the example of the course of the first stage of implantation. Materials and methods. Dental implants made of titanium with 3D Active and Xpeed surface treatment were used. The surface microstructure and biocompatibility of the samples, surface corrosion, elemental analysis of the surface was studied. The assessment of the severity of peri-implantitis and mucositis based on the depth of the peri-implant pocket and bleeding during probing, determination of the coefficient of stability of implants (KSI) is given. Median test (χ2), Kruskel–Wallis test (H), univariate variance analysis (F) were used for multiple comparison of samples. The difference in parameters was considered statistically significant at the p ≤ 0.05 level. Results. The surfaces of 3D Active implants are monolithic with rounded pores in the form of wells of different diameters and depths; the upper layer of the Xpeed sample consists of closely adjacent to each other fragments of a rectangular or polygonal shape, in the form of tiles, leaving rectangular faces on the outside. On the surface of the 3D Active samples, in addition to oxygen and carbon, there was phosphorus – 5.04 wt%, as well as S, Ca, Na, Zr, Zn which weight percentage did not exceed 1 wt%. A significant difference in the elemental composition of Xpeed implants was the presence of chlorine 0.07 wt% and 0.93 wt%, silicon – 0.10 wt% and 0.14 wt%, aluminum 0.06 wt% and 0.23 wt%, respectively, with the latter has a significant advantage in these elements. Xpeed had an insignificant weight percentage of iron in its composition – 0.12 wt%. The assessment of cell adhesion to the surface of the samples on the first day of incubation demonstrated that the samples with the Xpeed and 3D Active surfaces did not have a statistically significant difference between them. Also, on the 7th day of incubation, the level of reduction of resazurin in Xpeed was lower, no significant difference was observed (p > 0.05). The clinical signs of bone tissue resorption were identified in 36.4 % of 3D Active implants and 60.0 % of Xpeed implants (p = 0.3); inflammation of the mucous membrane – in the areas of installation of 54.5 % of 3D Active implants. No signs of mucositis were detected around the Xpeed implants (p = 0.23). 9.1 % of 3D Active implants were “lost”; no cases of loss of Xpeed implants have been identified. The KSI of 3D Active – 64.0 ± 5.9 units, Xpeed – 65.1 ± 3.7 units. Conclusions. According to KSI indicators, titanium implants with Xpeed coating (65.1 ± 3.7 units) and 3D Active (64.0 ± 5.9 units) showed the same capabilities. Bone tissue resorption without signs of inflammation is more common around Xpeed-coated implants than 3D Active-coated implants. Clinical signs of inflammation are more frequent and more severe around implants with a 3D Active coating, which affected both the severity of the course of the first stage of implantation and its results. Xpeed-coated implants are more reliable than 3D Active: loss among 3D Active implants was 9.1 %, Xpeed – 0.0 %.

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“…Stainless steel provides corrosion resistance, high-temperature sustainability, excellent mechanical strength, and durability. Titanium is also an excellent choice for geothermal heat exchangers due to its lightweight and outstanding corrosion resistance [2]. Material selection depends on the geothermal fluid chemistry influenced by geographical location and heat exchanger configuration.…”

Section: Introductionmentioning

confidence: 99%

Spray Parameters and Coating Microstructure Relationship in Suspension Plasma Spray TiO2 Coatings

Mittal,

Malik,

Bhuvanendran Nair Jayakumari

et al. 2023

Coatings

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In recent years, there has been growing interest in thermal spray techniques using suspension or solution-based coatings. These techniques offer precise control over particle size and microstructure, improving feedstock flowability and allowing for high-quality coating customization. Spray parameters, such as stand-off distance (SOD) and feedstock flow rate, can alter the performance and characteristics of these coatings. Geothermal power plant heat exchangers often face issues like corrosion, scaling, and fouling. The literature suggests that these issues could be mitigated, at least in part, by the use of spray coatings. In this study, TiO2 coatings were applied on a carbon steel substrate using suspension plasma spray (SPS) to enhance the performance of geothermal heat exchanger materials. The impact of SOD (50, 75, and 100 mm) and feedstock flow rate (10, 20, and 30 mL/min) on these coatings was examined through various techniques, including scanning electron microscope (SEM), profilometry, X-ray diffraction (XRD), and adhesion testing. The results demonstrated that coatings deposited using a 10 mL/min feedstock flow rate were well adhered to the substrate due to the efficient melting of the coating material, but as the SOD and feedstock flow rate increased due to poor thermal and kinetic energy exchange between the torch and feedstock particles, adhesion between the coating and substrate decreased.

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Preclinical EIS Study of the Inflammatory Response Evolution of Pure Titanium Implant in Hank’s Biological Solution

Cited by 9 publications

References 32 publications

Mitochondrial Redox Balance of Fibroblasts Exposed to Ti-6Al-4V Microplates Subjected to Different Types of Anodizing

Mitochondrial Redox Balance of Fibroblasts Exposed to Ti-6Al-4V Microplates Subjected to Different Types of Anodizing

Evaluation of anti-inflammatory properties on the surface of dental implants depending on the type of processing (Part 1)

Spray Parameters and Coating Microstructure Relationship in Suspension Plasma Spray TiO2 Coatings

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