Precocious induction of oocyte maturation and ovulation in rainbow trout (Salmo gairdneri) : problems when using 17 α-hydroxy-20 β-dihydroprogesterone

Jalabert, Bernard; Breton, Bernard; Fostier, Alexis; Reinaud, Pierrette; Heydorff, Micheline

doi:10.1051/rnd:19780534

Cited by 37 publications

(10 citation statements)

References 3 publications

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“…For instance, the active uptake and conversion of vitellogenin into yolk proteins followed by the packaging of these proteins into "yolk globules" (Khoo 1978;Wallace and Selman 1981) requires the production of enzymes. The final maturation of the ovaries is a process during which meiosis is resumed and germinal vesicle breakdown occurs (Jalabert et al 1978;Jalabert and Finet 1986). These processes also require specific proteins.…”

Section: Protein Metabolism In the Sexually Maturing Female Salmonmentioning

confidence: 99%

Protein metabolism during sexual maturation in female Atlantic salmon (Salmo salar L)

Martin

Houlihan

Talbot

et al. 1993

Fish Physiol Biochem

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Body composition and fractional rates of protein synthesis (percentage of the protein mass synthesized per day) were determined in female Atlantic salmon returning to the River Tay, Scotland in July and in October after a 95 day period without food, during which time the animals became sexually mature. During the 95 day period of starvation/sexual maturation the ventricle and red muscle remained as a constant proportion of fresh weight whereas the liver, gill and ovary increased and the stomach and white muscle decreased. Fractional rates of protein synthesis increased markedly in the liver, stomach and ovary during the period of starvation/sexual maturation. In the gill, ventricle and white muscle fractional protein synthesis rates increased slightly or remained constant. From the estimated rates of protein loss or gain in the various tissues it is concluded that there is considerable protein turnover and repartitioning of amino acids during the period of starvation and sexual maturation. The absolute rate of protein synthesis rates in the ovary indicates that this tissue made the largest contribution to the energy and amino acid demands of the fish, whilst most of the amino acids required for maturation of the ovary were derived from white muscle, principally as the result of increased muscle protein degradation.

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Section: Protein Metabolism In the Sexually Maturing Female Salmonmentioning

confidence: 99%

Protein metabolism during sexual maturation in female Atlantic salmon (Salmo salar L)

Martin

Houlihan

Talbot

et al. 1993

Fish Physiol Biochem

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“…Endocrine and paracrine signals are necessary for the coordination of the different follicular compartments and depend on the receptivity to MIS and gonadotropins, and for the modulation of intercellular communication such as that of gap junctions [1,7]. As long as this differentiation stage, or follicular maturational competence (FMC) [8] is not reached, a precocious stimulation of the oocyte by the MIS or of the follicle by the gonadotropins can result in no oocyte maturation, oocyte maturation not followed by ovulation [2,3,9], or ovulation of poor-quality oocytes exhibiting a poor developmental competence [9]. Thus, maturational competence implies that each cellular component of the follicle is able to receive and correctly translate an external signal (receptivity) within a normal physiological range (sensitivity) and is subsequently capable of giving the appropriate response (responsiveness) [1].…”

Section: Introductionmentioning

confidence: 99%

Targeted Gene Expression Profiling in the Rainbow Trout (Oncorhynchus mykiss) Ovary During Maturational Competence Acquisition and Oocyte Maturation

Bobe¹,

Tri²,

Jalabert³

2004

Biology of Reproduction

103

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A real-time polymerase chain reaction-based gene expression survey was performed using 37 target genes and 22 female rainbow trout sampled during follicular maturational competence (FMC) acquisition or during oocyte maturation. In females sampled before meiosis resumption, FMC was estimated using an in vitro assay. Several growth factors, bone morphogenetic proteins, steroidogenic enzymes, cathepsins, genes known to play a role in the fish preovulatory ovary, as well as previously unstudied genes, were analyzed in this survey. Gene expression profiling was performed using a supervised clustering analysis in order to identify groups of genes exhibiting similar expression profiles in the ovary during FMC acquisition and follicular maturation. From the clustering analysis, three clusters exhibiting a specific expression during FMC acquisition or at the time of oocyte maturation were identified. Cluster 1 was characterized by a progressive increase in gene expression during FMC acquisition, whereas cluster 2 exhibited an increased expression at the time of oocyte maturation. In contrast, cluster 3 was characterized by a decreased mRNA expression at the time of oocyte maturation. Among the 37 target genes used in this survey, 18 were significantly regulated during maturational competence acquisition or at the time of oocyte maturation. Among these 18 genes, 16 belonged to one of the three clusters identified. Although the results allowed a global description of gene expression profiles, they also suggest an important role for several factors, including some previously unstudied bone morphogenetic proteins, in the paracrine control of FMC acquisition and meiosis resumption.

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“…After manual stripping, the developmental stage of oocytes was assessed under a binocular microscope according to previously described criteria (Jalabert et al, 1978;Jalabert and Fostier, 1984).…”

Section: In Vivo Monitoring Of Oocyte Massmentioning

confidence: 99%

Hydration of rainbow trout oocyte during meiotic maturation andin vitroregulation by 17,20β-dihydroxy-4-pregnen-3-one and cortisol

Milla¹,

Jalabert²,

Rime³

et al. 2006

Journal of Experimental Biology

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Although oocytes of many teleost fish, especially marine species, are subjected to a hydration process during meiotic maturation, which leads to an important volume increase, no noticeable hydration of the preovulatory oocyte has ever been reported in rainbow trout (Oncorhynchus mykiss). In the present study, oocyte water content and dry mass were monitored using consecutive samples taken in vivo from the same female rainbow trout, from 4-5·days prior to ovulation to up to 7·days postovulation. In addition, yolk protein electrophoretic patterns were compared between oocytes sampled prior to germinal vesicle breakdown (GVBD) and unfertilized eggs. Furthermore, the effect of the maturation-inducing steroid (17,20␤ ␤-dihydroxy-4-pregnen-3-one, 17,20␤ ␤-P), cortisol and 11-deoxycorticosterone (DOC) on oocyte dry and wet masses, as well as GVBD occurrence was assessed in vitro. Finally, mRNA expression profiles of glucocorticoid and mineralocorticoid receptors as well as 11␤ ␤-hydroxysteroid dehydrogenase (11␤ ␤-HSD) were monitored in the periovulatory ovary by real-time PCR.Both in vivo and in vitro data showed, for the first time in rainbow trout, that a significant oocyte hydration occurs during oocyte maturation. In addition, an intra-oocyte dry matter increase was reported in vivo during the periovulatory period. However, yolk protein migration patterns were similar in preGVBD oocytes and unfertilized eggs, suggesting that no or little yolk proteolysis occurs during oocyte maturation. We also showed that oocyte hydration can be induced in vitro by 17,20␤ ␤-P and cortisol but not by DOC. In contrast, GVBD was only observed after 17,20␤ ␤-P stimulation. Finally, real-time PCR analysis showed an up-regulation of 11␤ ␤-HSD and glucocorticoid receptor 2 transcripts in the ovary at the time of oocyte maturation. Together, these results suggest that cortisol could participate in the control of oocyte hydration and possibly in other periovulatory ovarian functions.

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Precocious induction of oocyte maturation and ovulation in rainbow trout (Salmo gairdneri) : problems when using 17 α-hydroxy-20 β-dihydroprogesterone

Cited by 37 publications

References 3 publications

Protein metabolism during sexual maturation in female Atlantic salmon (Salmo salar L)

Protein metabolism during sexual maturation in female Atlantic salmon (Salmo salar L)

Targeted Gene Expression Profiling in the Rainbow Trout (Oncorhynchus mykiss) Ovary During Maturational Competence Acquisition and Oocyte Maturation

Hydration of rainbow trout oocyte during meiotic maturation andin vitroregulation by 17,20β-dihydroxy-4-pregnen-3-one and cortisol

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