Preconditioning of Nodal Explants in Thidiazuron-Supplemented Liquid Media Improves Shoot Multiplication in Pterocarpus marsupium (Roxb.)

Ahmad, Anees; Ahmad, Naseem; Anis, Mohammad

doi:10.1007/978-981-10-8004-3_8

Cited by 11 publications

(6 citation statements)

References 34 publications

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“…Another study by Huetteman and Preece [ 196 ] addressed the issue of shoot elongation by following a two-step culture strategy consisting of a primary medium fortified with TDZ for the induction of multiple shoot buds, followed by their transfer to a secondary medium fortified with another plant hormone (i.e., BA) that promotes shoot elongation. Such a type of culture strategy of using primary medium (shoot bud breaking/induction medium) and secondary medium (shoot elongation medium) has been successfully applied to a several tree species [ 137 , 147 , 197 , 198 , 199 ].…”

Section: Propagation Of Pterocarpus Marsupiummentioning

confidence: 99%

“…A successful and efficient regeneration protocol from the nodal segment of P. marsupium was established by Ahmad, Ahmad, and Anis [ 147 ]. The NS is a good source of axillary shoot multiplication in the tissue culture system, and even a single explant can give rise to multiple copies of true-to-type plantlets within a few months.…”

Section: Propagation Of Pterocarpus Marsupiummentioning

confidence: 99%

“…The stimulatory effect of IBA on induction of rooting may be due to successive rooting gene activation and better uptake, transportation, and stability compared with other auxins [ 159 , 273 ]. This type of two-step rooting strategy has been applied in many tree species [ 147 , 274 ]. In the first step, the distal ends of microshoots are dipped in a half-strength MS (liquid) medium containing a high dose of auxin (IBA) over a filter paper bridge for several days.…”

Section: Propagation Of Pterocarpus Marsupiummentioning

confidence: 99%

“…In the study by Ahmad and Anis [ 22 ], the hardening of regenerated plantlets of P. marsupium was achieved by the well-rooted shootlets being gently removed from the culture vessel and washed with running tap water to remove any adherent; after which the plantlets were transplanted to Thermocol cups (10-cm diameter) containing sterile Soilrite. Other researchers have covered the culture cups with transparent polyethylene bags as a safeguard to ensure high humidity and placed the cups under 16/8 h (day/night) in culture room conditions [ 147 , 301 ]. These polybags were gradually opened in order to acclimatize the plantlets to natural conditions.…”

Section: Propagation Of Pterocarpus Marsupiummentioning

confidence: 99%

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Biotechnological Advances in Pharmacognosy and In Vitro Manipulation of Pterocarpus marsupium Roxb.

Ahmad

Anis

et al. 2022

Plants

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Trees are vital resources for economic, environmental, and industrial growth, supporting human life directly or indirectly through a wide variety of therapeutic compounds, commodities, and ecological services. Pterocarpus marsupium Roxb. (Fabaceae) is one of the most valuable multipurpose forest trees in India and Sri Lanka, as it is cultivated for quality wood as well as pharmaceutically bioactive compounds, especially from the stem bark and heartwood. However, propagation of the tree in natural conditions is difficult due to the low percentage of seed germination coupled with overexploitation of this species for its excellent multipurpose properties. This overexploitation has ultimately led to the inclusion of P. marsupium on the list of endangered plant species. However, recent developments in plant biotechnology may offer a solution to the overuse of such valuable species if such advances are accompanied by technology transfer in the developing world. Specifically, techniques in micropropagation, genetic manipulation, DNA barcoding, drug extraction, delivery, and targeting as well as standardization, are of substantial concern. To date, there are no comprehensive and detailed reviews of P. marsupium in terms of biotechnological research developments, specifically pharmacognosy, pharmacology, tissue culture, authentication of genuine species, and basic gene transfer studies. Thus, the present review attempts to present a comprehensive overview of the biotechnological studies centered on this species and some of the recent novel approaches for its genetic improvement.

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Section: Propagation Of Pterocarpus Marsupiummentioning

confidence: 99%

Section: Propagation Of Pterocarpus Marsupiummentioning

confidence: 99%

Section: Propagation Of Pterocarpus Marsupiummentioning

confidence: 99%

Section: Propagation Of Pterocarpus Marsupiummentioning

confidence: 99%

See 2 more Smart Citations

Biotechnological Advances in Pharmacognosy and In Vitro Manipulation of Pterocarpus marsupium Roxb.

Ahmad

Anis

et al. 2022

Plants

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“…Due to these listed medicinal and commercial values, P. marsupium has gained a great deal of interest in recent years, and the Government of India has begun to promote a campaign for its large-scale production and conservation programmes. The propagating material of the species is only seed, but has a low germination rate (less than 30%) due to hard fruit coat coupled with a limited viability time and weak pod setting (Anis et al 2005;Husain et al 2010;Ahmad et al 2018). Pathogenic infections on seeds are also affecting their germination rate in natural condition (Yogeshwar et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Molecular Fingerprinting for Detecting Genetic Relationships among different Accessions of Pterocarpus marsupium

Ahmad¹

2021

IJAB

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Pterocarpus marsupium Roxb. is a valuable multipurpose forest tree in India. Generally, it is valued greatly for its excellent wood qualities. Due to its significant multipurpose properties, this tree has been overexploited, which ultimately has led to its inclusion in the list of threatened species. In this regard, studying the genetic diversity in P. marsupium is not only significant for the protection of this species, but also necessary for the development and utilization of germplasm resources for its improvement. Before developing any tree improvement program, information on actual genetic diversity and the cryptic number of the differentiated genetic resource are important aids for its conservation and effective utilization. Thus, in the present study, analysis of phylogenetic relationship among P. marsupium species plays an important role in the identification and selection of elite genotype among the wildly distributed accessions. The phylogenetic relationship among 18 genotypes obtained from various forest regions of central India was studied using DNA based molecular markers. In RAPD analysis, out of 40 scorable amplified bands, 29 were polymorphic resulting in expression of polymorphism percentage (73.2%) with an average of 2.90 amplicons per primer. Based on RAPD analysis, the lowest (37%) similarities among accessions were recorded in Anuppur (MAA), Mandla (MMK) and Jabalpur (MJH) and the highest similarity (100%) were observed among Mandla (MMK), Jablapur (MJH); Jashpur (CJM), Surguja (CSA), Bilaspur (CBP) and Durg (CDB) and Raigarh (CRK) accessions. While the ISSR analysis found 66 amplified bands, 45 were polymorphous and average 68.3 percent polymorphic with an average 4.5 bands per oligo. The lowest (36%) similarity was observed among Anuppur (MAA) and Jabalpur (MJH) accessions and the highest similarity (88%) was recorded among Jashpur (CJM), Chhindwara (MCD) and Bilaspur (CBP) accessions. The combined analysis data of RAPD and ISSR showed that Chandrapur (RCC) and Anuppur's (MAA) acessions had the lowest (35%) similarity, with Jabalpur's (MJHs) and Mandla's (MMKs) accession being the highest similarities (100%) reported. As a result, the study of genetic diversity by means of RAPD and ISSR markers alone or in combination, i.e. the MAA, CKB and CRK accessions, was found to be more diverse among 18 accessions of Central India and given greater space for the collection of elite/superior trees to be used in conservation and forest development programs. © 2021 Friends Science Publishers

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Selection of Elite Genotype of a Multipurpose Forest Tree (Pterocarpus marsupium Roxb.) from Naturally Grown Populations in Central India

Ahmad

Siddique

Varshney

2020

Propagation and Genetic Manipulation of Plants

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Preconditioning of Nodal Explants in Thidiazuron-Supplemented Liquid Media Improves Shoot Multiplication in Pterocarpus marsupium (Roxb.)

Cited by 11 publications

References 34 publications

Biotechnological Advances in Pharmacognosy and In Vitro Manipulation of Pterocarpus marsupium Roxb.

Biotechnological Advances in Pharmacognosy and In Vitro Manipulation of Pterocarpus marsupium Roxb.

Molecular Fingerprinting for Detecting Genetic Relationships among different Accessions of Pterocarpus marsupium

Selection of Elite Genotype of a Multipurpose Forest Tree (Pterocarpus marsupium Roxb.) from Naturally Grown Populations in Central India

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