Precursor Supply Strategy for Tetramethylpyrazine Production by Bacillus Subtilis on Solid-State Fermentation of Wheat Bran

Hao, Fei Lin; Wu, Qun; Xu, Yan

doi:10.1007/s12010-012-0083-0

Cited by 31 publications

(23 citation statements)

References 22 publications

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“…Bacillus species can also generate volatile compounds such as C 4 compounds, pyrazines, volatile acids, and aromatic and phenolic compounds in the liquor. These provide an improved sensory effect and various health benefits [5,34]. However, the functionality of the Bacillus species in Baiyunbian Daqu requires further investigation.…”

Section: Resultsmentioning

confidence: 99%

PCR-DGGE Analysis of the Microbial Communities in Three Different Chinese ��Baiyunbian�� Liquor Fermentation Starters

Xiong

Hu²,

Yan³

et al. 2014

Journal of Microbiology and Biotechnology

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A systematic investigation was performed on the bacterial, Bacillus, fungal, and yeast communities of the three types of Daqu (mechanically prepared, manually prepared, and mixed prepared) used in Baiyunbian Company by reconditioning PCR-denaturing gradient gel electrophoresis (PCR-DGGE). The DGGE results showed that the microbes in the three types of Daqu were mainly thermotolerant and thermophilic microbes, and the most dominant bacterial species were Bacillus and Virgibacillus, followed by Lactobacillus and Trichococcus. Furthermore, the dominant fungi were found to be molds, such as Rasamsonia, Penicillium, Aspergillus, and Monascus, and the dominant yeasts were Saccharomyces cerevisiae, Saccharomycopsis fibuligera, Pichia anomala, and Debaryomyces hansenii. In general, the three types of Daqu showed slight differences in microbial communities, and the Shannon indexes (H') of the manually prepared and mechanically prepared Daqu were similar. The results suggest that mechanically prepared Daqu can replace manually prepared Daqu in liquor production, and this research provides useful information for liquor production and process improvement.

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Section: Resultsmentioning

confidence: 99%

PCR-DGGE Analysis of the Microbial Communities in Three Different Chinese ��Baiyunbian�� Liquor Fermentation Starters

Xiong

Hu²,

Yan³

et al. 2014

Journal of Microbiology and Biotechnology

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show abstract

“…During the first step, acetoin was efficiently biosynthesized from glucose. If DAP had been added into the fermentation medium in this step, excess salts would cause serious inhibition of cell growth and acetoin accumulation [24]. A DAP feeding strategy was applied to deal with this problem [13].…”

Section: Comparison Of Tmp Production Methods From Renewable Feedstocksmentioning

confidence: 99%

Accelerated green process of tetramethylpyrazine production from glucose and diammonium phosphate

Xiao

Hou

Lyu

et al. 2014

Biotechnol Biofuels

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“…Pharmacological studies have demonstrated the ability of TMP to inhibit platelet aggregation, mediate vasodilation, and enhance coronary blood flow. Besides, this compound is widely employed as a flavor additive in the culinary industry 5,6 , and also used as a standard compound in many other industries 7 .…”

mentioning

confidence: 99%

Enhanced Production of Tetramethylpyrazine in Bacillus licheniformis BL1 through aldC Over-expression and acetaldehyde Supplementation

Meng

Ding

Wang

et al. 2020

Sci Rep

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modification. Through strengthening the degradation process and blocking the competing pathways, the carbon flux can flow to acetoin biosynthesis pathway. The acetoin then was accumulated to bolster TMP production. Glucose-derived TMP yields were higher for engineered strains relative to the initial controls. We additionally explored a novel means of adding supplemental acetaldehyde during fermentation, thereby enhancing TMP yields while keeping the overall cost low. Materials and Methods Cells and reagents. The strains, plasmids and their relevant genotypes for this study are compiled in Table 1. Table 2 lists all the PCR primers in this study. All DNA manipulation was conducted via standard approaches. Escherichia coli was cultured at 37 °C with LB medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract) plus ampicillin (100 μg/mL) for transformant selection. During acetoin and TMP fermentation, 70 g/L glucose was supplemented into the LB broth, and every 12 hours we added 5 mL of the supplement (1 mg/mL glucose). In addition, LB plates containing 100 μg/mL filter-sterilized Ampicillin antibiotic were employed for B. lincheniformis transformant selection of Amp resistant strains. Solid medium was prepared via the addition of 20 g/L agar. Recombinant strain production. The oligonucleotides listed in Table 2 were used to construct plasmids (Fig. 1) as a means of preparing a genome integration cassette. First, B. lincheniformis BL1 gDNA was used for the amplification of a 1739 bp CDS region of aldC and a 2462 bp CDS region of alsS using the aldC-F1/aldC-R2 and alsS-F1/alsS-R2 primer pairs, respectively. The PCR products were digested with EcoRV/ EcoRI and BsrGI/ NcoI, respectively, and then inserted into PMA5.1 to construct the PMA5.1-aldC plasmid (Fig. 1a) and the PMA5.1-alsS plasmid (Fig. 1b), respectively. Finally, the CDS region of aldC and the CDS region of alsS were cloned into the plasmid PMA5.1 to construct the PMA5.1-aldC-alsS plasmid. The restriction sites were EcoRV/ EcoRI and BsrGI/ NcoI (Fig. 1c).

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Precursor Supply Strategy for Tetramethylpyrazine Production by Bacillus Subtilis on Solid-State Fermentation of Wheat Bran

Cited by 31 publications

References 22 publications

PCR-DGGE Analysis of the Microbial Communities in Three Different Chinese ��Baiyunbian�� Liquor Fermentation Starters

PCR-DGGE Analysis of the Microbial Communities in Three Different Chinese ��Baiyunbian�� Liquor Fermentation Starters

Accelerated green process of tetramethylpyrazine production from glucose and diammonium phosphate

Enhanced Production of Tetramethylpyrazine in Bacillus licheniformis BL1 through aldC Over-expression and acetaldehyde Supplementation

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