Predictable and linear scale-up of four phenolic alkaloids separation from the roots of Menispermum dauricum using high-performance counter-current chromatography

Luo, Houding; Peng, Ming; Ye, Haoyu; Chen, Lijuan; Peng, Aihua; Tang, Minghai; Zhang, Fan; Shi, Jie

doi:10.1016/j.jchromb.2010.05.002

Cited by 19 publications

(14 citation statements)

References 13 publications

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“…The free-space V 2 and thus the scale-up factor are directly calculated on the rotor experimental behavior observations. Therefore, it is not necessary to restrain operating parameters to identical gravitational field or stationary phase retention ratio as observed in the literature [7,22,23] except temperature which is discussed later. The proposed "free-space between peaks" method allows the user to choose rotational speed and flow rate conditions according to its own specifications (pressure, solvent costs, duration and reducing equipment wear).…”

Section: Analytical Injection In the 239-ml Rotor: Free-space Betweenmentioning

confidence: 99%

“…Representation of the separation of two Gaussian peaks illustrating the concept of "free space between peaks", V, compared to the retention difference, Vr, used in the definition of the chromatographic resolution. Luo [7] performed an analytical separation of four phenolic alkaloids using an 18-mL CCC column. He then transferred it on a 50-times larger 900-mL column.…”

Section: Introductionmentioning

confidence: 99%

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Scale-up in centrifugal partition chromatography: The “free-space between peaks” method

Bouju

Berthod

Faure

2015

Journal of Chromatography A

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Centrifugal Partition Chromatography (CPC) is a purification technique using a biphasic liquid system. As a preparative separation technique, scale-up is of primary concern. Once the separation is optimized on a lab-scale instrument, the scale-up transfer is quite straightforward simply using the instrument volume ratio as the linear transfer factor, thanks to the absence of solid support. Such linear transfer underestimates the performances of large-scale CPC rotors that are usually better than that of small rotors. It means that more material than predicted by the linear estimation could be purified. A fully practical method based on experimental observations is proposed. The first step is to determine experimentally the free space volume available between the two peaks of interest doing two analytical separations, one with the small analytical CPC instrument, giving ΔV1, and the second with the large preparative one, giving ΔV2. The second step is to determine on the small CPC instrument how much material can be loaded to reach the maximum mass load still giving the required purity and recovery ratio of the desired compound. Then, an accurate prediction of the maximal quantity of sample that the large-scale rotor can purify is simply obtained by multiplying the maximum mass load on the analytical CPC instrument by the free-space between peaks ΔV2/ΔV1 ratio. For demonstration purposes, the method is applied to the transfer of the CPC separation of a synthetic three-GUESS-compound mixture from a 35mL-rotor to a semi-prep 239-mL rotor. The paper addresses also the operating condition optimization depending on industrial production strategy (maximal load per run or maximal productivity).

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Section: Analytical Injection In the 239-ml Rotor: Free-space Betweenmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Scale-up in centrifugal partition chromatography: The “free-space between peaks” method

Bouju

Berthod

Faure

2015

Journal of Chromatography A

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“…Analytes with different K values in a solvent system may be separated by CCS with that solvent system [5]. The loading material may be a standard sample [6][7][8] or a crude material [9][10][11][12][13][14][15][16]. Such experiments have been described in detail [6,16].…”

Section: Partitioning Experimentsmentioning

confidence: 99%

Solvent System Selection Strategies in Countercurrent Separation

et al. 2015

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The majority of applications in countercurrent and centrifugal partition chromatography, collectively known as countercurrent separation, are dedicated to medicinal plant and natural product research. In countercurrent separation, the selection of the appropriate solvent system is of utmost importance as it is the equivalent to the simultaneous choice of column and eluent in liquid chromatography. However, solvent system selection is often laborious, involving extensive partition and/or analytical trials. Therefore, simplified solvent system selection strategies that predict the partition coefficients and, thus, analyte behavior are in high demand and may advance both the science of countercurrent separation and its applications. The last decade of solvent system selection theory and applications are critically reviewed, and strategies are classified according to their data input requirements. This offers the practitioner an up-to-date overview of rationales and methods for choosing an efficient solvent system, provides a perspective regarding their accuracy, reliability, and practicality, and discusses the possibility of combining multiple methods for enhanced prediction power.

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“…It was run at a speed of 1800 rpm (181 Â g). This instrument provides higher b range 0.68-0.79 and rotor speed than normal HSCCC, which results in high resolution and throughput (16).…”

Section: Apparatusmentioning

confidence: 99%

“…Compared with the common HSCCC, this HPCCC can provide five-fold high ''g'' values and higher flow rates with nearly the same stationary phase retention (S f ) and separation time as analytical HPCCC. Thus, the linear scale-up is easy to achieve after simple calculation (16,18).…”

Section: Parameters Optimizationmentioning

confidence: 99%

Separation and Purification of Quinolone Alkaloids from the Chinese Herbal MedicineEvodia rutaecarpa (Juss.)Benth by High Performance Counter-Current Chromatography

Zhong

Peng

et al. 2011

Separation Science and Technology

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Predictable and linear scale-up of four phenolic alkaloids separation from the roots of Menispermum dauricum using high-performance counter-current chromatography

Cited by 19 publications

References 13 publications

Scale-up in centrifugal partition chromatography: The “free-space between peaks” method

Scale-up in centrifugal partition chromatography: The “free-space between peaks” method

Solvent System Selection Strategies in Countercurrent Separation

Separation and Purification of Quinolone Alkaloids from the Chinese Herbal MedicineEvodia rutaecarpa (Juss.)Benth by High Performance Counter-Current Chromatography

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