Predicting associations between microRNAs and target genes in breast cancer by bioinformatics analyses

Zheng, Ting; Zhang, Xing; Wang, Yonggang; Yu, Xiucui

doi:10.3892/ol.2016.4731

Cited by 16 publications

(11 citation statements)

References 34 publications

(30 reference statements)

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“…BCa patients are likely to develop tumor recurrence and metastasis after surgical treatment [18]. In recent years, miRNAs in BCa research have attracted increasing research attention [7,19], for example studies on the interaction between miRNA with related target genes and the regulative role of signaling pathways revealed the critical effects of miRNAs on tumors [20,21]. Thus, discovering clinical targets indicating the prognosis of BCa may contribute to the treatment of BCa.…”

Section: Discussionmentioning

confidence: 99%

MiR-942 regulates the function of breast cancer cell by targeting FOXA2

Zhang¹,

Zhang²,

Sun³

et al. 2019

Bioscience Reports

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MicroRNA (MiR)-942 regulates the development of a variety of tumors, however, its function in breast cancer (BCa) has been less reported. Therefore, the present study investigated the regulatory effects of miR-942 on BCa cells. The expression of miR-942 in whole blood samples and BCa cell lines was detected by quantitative real-time (qRT)-PCR. Direct target gene for miR-942 was confirmed by dual-luciferase reporter assay. FOXA2 expression in adjacent tissues was detected by qRT-PCR. The effects of miR-942, or miR-942 with FOXA2, on the cell viability, proliferation, apoptosis, migration and invasion of BCa cells were determined by cell counting kit-8 (CCK-8), colony formation assay, flow cytometry, wound scratch and Transwell, respectively. The levels of N-Cadherin, E-Cadherin and Snail were determined by Western blot. Kaplan–Meier was used to explore the relationship among the expressions of miR-942 and FOXA2 and the prognosis of BCa patients. MiR-942 had high expressed in BCa, while its low expression significantly suppressed the cell viability, proliferation, migration and invasion of BCa, but increased cell apoptosis. Down-regulation of N-Cadherin and Snail and up-regulation of E-Cadherin were also induced by low-expression of miR-942. FOXA2, which was proved as the direct target gene for miR-942 and was low-expressed in BCa, partially reversed the effect of overexpressed miR-942 on promoting cell viability, proliferation, migration and invasion, and suppressed cell apoptosis. A lower survival rate was observed in BCa patients with a high expression of miR-942 and a low expression of FOXA2. MiR-942 promoted the progression of BCa by down-regulating the expression of FOXA2.

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Section: Discussionmentioning

confidence: 99%

MiR-942 regulates the function of breast cancer cell by targeting FOXA2

Zhang¹,

Zhang²,

Sun³

et al. 2019

Bioscience Reports

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“…Using microarray and PCR, we found a significant downregulation of miR-143 and miR-145 in malignant tumors compared to normal tissue, which is in line with previously published studies in breast cancer [ 29 – 31 ] and other tissues, especially colorectal carcinomas [ 32 ]. However, in our study the results were also verified by ISH, which added interesting data on the cellular and subcellular location of these miRNAs in benign and malignant breast tissue.…”

Section: Discussionsupporting

confidence: 91%

Expression and function of the miR-143/145 cluster in vitro and in vivo in human breast cancer

et al. 2017

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MicroRNAs (miRNAs) are small non-coding RNAs that function as post-transcriptional regulators of gene expression and are dysregulated in cancer. Studies of miRNAs to explore their potential as diagnostic and prognostic markers are of great scientific interest. Here, we investigate the functional properties and expression of the miR-143/145 cluster in breast cancer (BC) in vitro and in vivo. The ER positive MCF7, the HER2 positive SK-BR-3, and the triple negative cell line MDA-MB-231 were used to assess cell proliferation and cell invasion. Expression of miRNA in 108 breast cancers in the Norwegian Women and Cancer Study and 44 benign tissue controls were analyzed by microarray and validated by RT-PCR. Further, in situ hybridization (ISH) was used to study the cellular and subcellular distribution of the miRNAs. In vitro, miR-143 promoted proliferation of MCF7 and MDA-MB-231 cells, whereas miR-145 and the cotransfection of both miRNAs inhibited proliferation in all three cell lines. The cells’ invasive capacity was reduced after transfection and cotransfection of the miRNAs. In line with the tumor suppressive functions in vitro, the expression of miR-143 and miR-145 was lower in malignant compared to benign breast tissue, and lower in the more aggressive tumors with higher tumor grade, loss of ER and the basal-like phenotype. ISH revealed miR-143 to be cytoplasmatic and predominantly expressed in luminal cells in benign tissue, whilst miR-145 was nuclear and with strong staining in myoepithelial cells. Both miRNAs were present in malignant epithelial cells and stromal fibroblasts in BC. This study demonstrates that miR-143 and -145 have functional properties and expression patterns typical for tumor suppressors, but the function is influenced by cellular factors such as cell type and miRNA cotransfection. Further, the nuclear functions of miR-145 should be explored for a more complete understanding of the complexity of miRNA regulation and function in BC.

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“…It reported that microRNA feedback loops induced apoptosis in breast cancer and colorectal cancer 14,15. A study showed that 13 miRNAs were expressed in breast cancer tissues and serum, but they showed variations.16 Thus, miRNAs may be released into the blood via breast cancer cells and be involved in different molecular mechanisms in tissues and serum. There were 1096 patients with breast cancer and 112 controls in the TCGA cohort (Figure 1A and B).…”

Section: Discussionmentioning

confidence: 99%

<p>miR-937 regulates the proliferation and apoptosis via targeting APAF1 in breast cancer</p>

Fang

Jiang

Jing

et al. 2019

OTT

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Background: Previous research had shown that an imbalance in cell proliferation and apoptosis is a vital mechanism for tumorigenesis and cancer progression that may directly influence biological behaviors of cancer. microRNAs are associated with the occurrence and development of tumors. This study aimed to explore the influence of miR-937 on breast cancer regulation of APAF1 expression. Methods: Cancer Genome Altas microarray analysis (fold change > 2, p<0.05) was used to verify differentially expressed microRNAs and RT-qPCR was used to detect miR-937 mRNA level in breast cancer. Cell viability and proliferation were measured using CCK8 and colony formation assays, respectively, after the miR-937 mimics/inhibitors and their negative control were transfected into MCF7 cells. The variations in cell cycle and apoptosis were examined using flow cytometry. DAVID database was used to perform GO enrichment analysis. We use dual luciferase report system to detect the effect of miR-937 on the transcriptional activity of APAF1. APAF1 protein level was determined by Western blot assay. Results: miR-937 was up-regulated in breast cancer cell lines and high miR-937 expression is associated with a poorer survival rate in cancer patients. miR-937 overexpression promoted the viability, down-regulated the G1 phase ratios and increased the ability of colony formation in breast cancer cells. miR-937 inhibition inhibited the viability and the ability of colony formation, promoted the apoptosis and up-regulated the G1 phase ratios. Our results showed that miR-937 targeted bind to the APAF1-3′UTR. APAF1 overexpression inhibited the viability and the ability of colony formation, promoted the apoptosis and up-regulated the G1 phase ratios. After cells were co-transfection miR-937 mimics and APAF1, cell apoptosis level was increased. Conclusion: APAF1 up-regulation or APAF1 down-regulation in breast cancer may regulate cell proliferation and apoptosis.

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Predicting associations between microRNAs and target genes in breast cancer by bioinformatics analyses

Cited by 16 publications

References 34 publications

MiR-942 regulates the function of breast cancer cell by targeting FOXA2

MiR-942 regulates the function of breast cancer cell by targeting FOXA2

Expression and function of the miR-143/145 cluster in vitro and in vivo in human breast cancer

<p>miR-937 regulates the proliferation and apoptosis via targeting APAF1 in breast cancer</p>

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