Predicting Pregnancy after Surgery for Intestinal Endometriosis: A Multivariate Analysis Based on Objective Clinical and Surgical Data

Abstract

myoma. Individual fibroids and blood of all patients were collected during the surgery. DNA extraction and amplification were carried out, the PCR reaction was conducted, and the sequence fragments were identified. Measurements/Results: 77 patients were included in the study (51 and 26 in the first and the second groups, respectively). 167 nodules were collected in patients of the first group. 74.5 % of women in the first group (38/51) were found to have mutations in an exon 2 of the gene MED 12. This mutation was found in 91 out of 167 nodules (54.5 %) of this group. 50% of women in the second group (13/26) were found to have the same somatic mutations in MED 12, demonstrating statistical significance among the groups of patients with studied mutations (p = .042). It was interesting to discover insertion at length 6 n. p. localized in the exon 2 gene MED 12, which has not been published in previous studies. Conclusions: We revealed that women with multiple leiomyoma exhibit mutations in an exon 2 of the gene MED 12 with greater frequency than women with single fibroid. We may propose that somatic mutations in exon 2 of the gene MED 12 can stimulate the affiliate multipotent cells and trigger the migration of their affiliate cells with the subsequent development of multiple leiomyomas.