The present study aims to enrich the research on online entrepreneurship education, improve the level and ability of entrepreneurship services in China, and improve the overall success rate of entrepreneurship. Based on the technology acceptance model (TAM) proposed by researcher Davis, the study explores the application of TAM in online entrepreneurship education for new entrepreneurs. First, new entrepreneurs who are users of the online entrepreneurship education platform are selected as research objects, and then the influence of the four factors are studied, including perceived ease of use, perceived usefulness, classroom self-efficacy, and perceived external control. Finally, the hypothesis proposed is tested. Results show that online entrepreneurship education influences the perceived ease of use of the user and perceived credibility: the quality of the online entrepreneurship education has a positive impact on the user's perceived usefulness, interactivity has a positive influence on perceived ease of use and perceived usefulness, and interactivity positively influences the perceived ease of use of the user and perceived credibility. Perceived usefulness, perceived ease of use, and perceived credibility have a positive impact on the behavioral intention of the users. The proposed TAM provides some technical and theoretical support for the application of TAM in online entrepreneurship education for new entrepreneurs.