Predicting the presence and titre of rabies virus‐neutralizing antibodies from low‐volume serum samples in low‐containment facilities

Meza, Diana K.; Broos, Alice; Becker, Daniel J.; Behdenna, Abdelkader; Willett, Brian J.; Viana, Mafalda; Streicker, Daniel G.

doi:10.1111/tbed.13826

Cited by 8 publications

(7 citation statements)

References 70 publications

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“…Points represent the date of (re)capture with the serostatus at that time in different colours. The dashed line represents the cutoff defining seropositivity based on [ 29 ]. (Online version in colour.)…”

Section: Resultsmentioning

confidence: 99%

“…Sera from 2007–2010 were tested for rabies virus neutralizing antibodies (RVNAs) using a micro-neutralization rapid fluorescent focus inhibition test (micro-RFFIT) [ 4 ]. Sera from 2011–2017 were tested using a pseudotype micro-RFFIT (pmRFFIT) which avoided use of live rabies virus and enabled direct quantification of RVNA titres [ 29 ]. We used a cutoff of 0.166 IU ml −1 to define seropositivity on tested samples by pmRFFIT which was previously shown to maximize the balance of sensitivity and specificity [ 29 ].…”

Section: Methodsmentioning

confidence: 99%

“…Sera from 2011–2017 were tested using a pseudotype micro-RFFIT (pmRFFIT) which avoided use of live rabies virus and enabled direct quantification of RVNA titres [ 29 ]. We used a cutoff of 0.166 IU ml −1 to define seropositivity on tested samples by pmRFFIT which was previously shown to maximize the balance of sensitivity and specificity [ 29 ]. Serological results in tested samples by both methods ( n = 2365) are 93.7% similar [ 29 ].…”

Section: Methodsmentioning

confidence: 99%

“…We used a cutoff of 0.166 IU ml −1 to define seropositivity on tested samples by pmRFFIT which was previously shown to maximize the balance of sensitivity and specificity [ 29 ]. Serological results in tested samples by both methods ( n = 2365) are 93.7% similar [ 29 ].…”

Section: Methodsmentioning

confidence: 99%

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Ecological determinants of rabies virus dynamics in vampire bats and spillover to livestock

et al. 2022

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The pathogen transmission dynamics in bat reservoirs underpin efforts to reduce risks to human health and enhance bat conservation, but are notoriously challenging to resolve. For vampire bat rabies, the geographical scale of enzootic cycles, whether environmental factors modulate baseline risk, and how within-host processes affect population-level dynamics remain unresolved. We studied patterns of rabies exposure using an 11-year, spatially replicated sero-survey of 3709 Peruvian vampire bats and co-occurring outbreaks in livestock. Seroprevalence was correlated among nearby sites but fluctuated asynchronously at larger distances. A generalized additive mixed model confirmed spatially compartmentalized transmission cycles, but no effects of bat demography or environmental context on seroprevalence. Among 427 recaptured bats, we observed long-term survival following rabies exposure and antibody waning, supporting hypotheses that immunological mechanisms influence viral maintenance. Finally, seroprevalence in bats was only weakly correlated with outbreaks in livestock, reinforcing the challenge of spillover prediction even with extensive data. Together our results suggest that rabies maintenance requires transmission among multiple, nearby bat colonies which may be facilitated by waning of protective immunity. However, the likelihood of incursions and dynamics of transmission within bat colonies appear largely independent of bat ecology. The implications of these results for spillover anticipation and controlling transmission at the source are discussed.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Ecological determinants of rabies virus dynamics in vampire bats and spillover to livestock

et al. 2022

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“…Images taken from the immunofluorescence assay were quantified using an image processing protocol 34 . Briefly, plates were photographed at 10X objective (70% brightness) under a fluorescent microscope (AMG EVOSfl, Thermo Fisher Scientific Inc, Waltham, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

Optimization in the expression of ASFV proteins for the development of subunit vaccines using poxviruses as delivery vectors

Lopera-Madrid

Medina-Magües

Gladue

et al. 2021

Sci Rep

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African swine fever virus (ASFV) causes a highly contagious hemorrhagic disease that affects domestic pig and Eurasian wild boar populations. To date, no safe and efficacious treatment or vaccine against ASF is available. Nevertheless, there are several reports of protection elicited by experimental vaccines based on live attenuated ASFV and some levels of protection and reduced viremia in other approaches such as DNA, adenovirus, baculovirus, and vaccinia-based vaccines. Current ASF subunit vaccine research focuses mainly on delivering protective antigens and antigen discovery within the ASFV genome. However, due to the complex nature of ASFV, expression vectors need to be optimized to improve their immunogenicity. Therefore, in the present study, we constructed several recombinant MVA vectors to evaluate the efficiency of different promoters and secretory signal sequences in the expression and immunogenicity of the p30 protein from ASFV. Overall, the natural poxvirus PrMVA13.5L promoter induced high levels of both p30 mRNA and specific anti-p30 antibodies in mice. In contrast, the synthetic PrS5E promoter and the S E/L promoter linked to a secretory signal showed lower mRNA levels and antibodies. These findings indicate that promoter selection may be as crucial as the antigen used to develop ASFV subunit vaccines using MVA as the delivery vector.

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Pseudotyped Viruses for Lyssavirus

Wang

Long

Wang

et al. 2023

Advances in Experimental Medicine and Biology

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Predicting the presence and titre of rabies virus‐neutralizing antibodies from low‐volume serum samples in low‐containment facilities

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 8 publications

References 70 publications

Ecological determinants of rabies virus dynamics in vampire bats and spillover to livestock

Ecological determinants of rabies virus dynamics in vampire bats and spillover to livestock

Optimization in the expression of ASFV proteins for the development of subunit vaccines using poxviruses as delivery vectors

Pseudotyped Viruses for Lyssavirus

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