Prediction and validation of potential pathogenic microRNAs involved in Phytophthora infestans infection

Cui, Juanjuan; Luan, Yushi; Wang, Weichen; Zhai, Junmiao

doi:10.1007/s11033-014-3037-5

Cited by 14 publications

(11 citation statements)

References 34 publications

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“…After 10 days, the cellophane, which was filled with P. infestans, was transferred to a new culture dish. Tomato leaves were used to cover the upper and lower sides of this cellophane as described previously31. P. infestans s amples were collected from the cellophane at 0, 3, 12, 24, 36, 48 and 72 hpi.…”

Section: Methodsmentioning

confidence: 99%

“…MiR1918 is expressed in all tomato tissues, and it was initially thought to be involved in the regulation of fruit development and maturation30. Recently, a number of P. infestans miRNAs (pi-miRNAs) have been predicted using P. infestans EST data and all the known miRNAs from miRBase, and pi-miR1918 is among these predicted pi-miRNAs, and its level was found to change dramatically in P. infestans -infected Solanum pimpinellifolium leaves31. In addition, a recent silico study has shown that tomato miR1918 might bind to the AC1 gene coding the replication-related protein in Tomato leaf curl virus (ToLCV).…”

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confidence: 99%

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MiR1918 enhances tomato sensitivity to Phytophthora infestans infection

Luan

Cui

Wang

et al. 2016

Sci Rep

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Late blight of tomato is caused by the oomycete pathogen Phytophthora infestans. In our previous work, we identified and characterized a miR1918 in P. infestans (pi-miR1918), and showed that its sequence is similar to the sequence of tomato miR1918 (sly-miR1918). In this study, we used Arabidopsis thaliana pre-miR159a as a backbone to synthesize pi-miR1918 via PCR and mutagenesis. The artificial pi-miR1918 was used to investigate the role of miR1918 in tomato-P. infestans interaction. Trangenic tomato plants that overexpressed the artificial pi-miR1918 displayed more serious disease symptoms than wild-type tomato plants after infection with P. infestans, as shown by increased number of necrotic cells, lesion sizes and number of sporangia per leaf. The target genes of pi-miR1918 and sly-miR1918 were also predicted for tomato and P. infestans, respectively. qPCR analysis of these targets also performed during tomato-P. infestans interaction. The expression of target gene, RING finger were negatively correlated with miR1918 in the all Lines of transgenic tomato plants. In addition, we used the 5′ RACE to determine the cleavage site of miR1918 to RING finger. These results suggested that miR1918 might be involved in the silencing of target genes, thereby enhancing the susceptibility of tomato to P. infestans infection.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

MiR1918 enhances tomato sensitivity to Phytophthora infestans infection

Luan

Cui

Wang

et al. 2016

Sci Rep

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“…The pathogen P. infestans was cultured according to Cui et al (2014). P. nicotianae was cultured at 28°C for 2 weeks in carrot medium.…”

Section: Pathogen Response Assaysmentioning

confidence: 99%

Sp-miR396a-5p acts as a stress-responsive genes regulator by conferring tolerance to abiotic stresses and susceptibility to Phytophthora nicotianae infection in transgenic tobacco

2015

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Overexpression of Sp-miR396a-5p in tobacco increased tolerance to salt, drought, cold stress and susceptibility to Phytophthora nicotianae infection. MicroRNA396 (miR396) is one of the conserved microRNA families in plants, and it targeted growth-regulating factors (GRFs) family. The GRF transcription factors are associated with growth and stress responses. However, the molecular mechanisms of miR396 responding to environmental stresses are elusive. The purpose of this study was to explore the function of tomato miR396a-5p (Sp-miR396a-5p) in Solanaceae responses to abiotic and biotic stresses. We showed that Sp-miR396a-5p transcript levels were up-regulated under salt and drought stresses and down-regulated after Phytophthora infestans (P. infestans) infection. Consistently, overexpression of Sp-miR396a-5p in tobacco enhanced its tolerance to salt, drought and cold stresses. Additionally, the expression of Sp-miR396a-5p was found to be down-regulated under pathogen-related biotic stress. Tobacco plants overexpressing Sp-miR396a-5p showed increased susceptibility to Phytophthora nicotianae (P. nicotianae) infection. Physiological analysis indicated that Sp-miR396a-5p overexpression enhanced osmoregulation and decreased production of reactive oxygen species (ROS). Furthermore, four Sp-miR396a-5p target genes, NtGRF1, NtGRF3, NtGRF7 and NtGRF8, were down-regulated in these plants. Our results suggested that Sp-miR396a-5p plays critical roles in both abiotic stresses through targeting NtGRF7-regulated expression of osmotic stress-responsive genes and pathogen infection via the regulatory networks of NtGRF1 and NtGRF3.

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“…P. infestans was cultured in oat medium according to the method described by Cui, Luan, Wang, and Zhai (). B. cinerea was cultured on potato dextrose agar medium supplemented with 20 g L −1 sucrose in the dark at 20°C for 12 hr.…”

Section: Methodsmentioning

confidence: 99%

“…Polymerase chain reaction was used to amplify the cDNA fragments of the 2.7 | Pathogens culture and pathogenicity assays P. infestans was cultured in oat medium according to the method described by Cui, Luan, Wang, and Zhai (2014). B. cinerea was cultured on potato dextrose agar medium supplemented with 20 g L −1 sucrose in the dark at 20°C for 12 hr.…”

Section: Virus-induced Gene Silencing (Vigs)mentioning

confidence: 99%

Solanum lycopersicum microRNA1916 targets multiple target genes and negatively regulates the immune response in tomato

Chen

Meng

et al. 2018

Plant Cell & Environment

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MicroRNA1916 (miR1916) is one of the nonconserved miRNAs that respond to various stresses in plants, but little has been known at present about its mechanisms in biotic stresses. In this study, the expression of Solanum lycopersicum (sly)‐miR1916 in tomato was found to be down‐regulated after infection with Phytophthora infestans or Botrytis cinerea. Tomato plants that overexpressed sly‐miR1916 displayed significant enhancement in susceptibility to P. infestans and B. cinerea infection, as well as increased tendency to produce reactive oxygen species. Silencing of sly‐miR1916 by short tandem target mimic and artificial microRNA strategies caused the tomato plants to become more tolerant to adverse conditions. In addition, lower sly‐miR1916 expression could up‐regulate the expression of strictosidine synthase (STR‐2), UDP‐glycosyltransferases (UGTs), late blight resistance protein homolog R1B‐16, disease resistance protein RPP13‐like, and MYB transcription factor (MYB12), which ultimately resulted in the accumulation of α‐tomatine and anthocyanins via STR‐2, UGT, and MYB12. Furthermore, ectopic expression of sly‐miR1916/STR‐2 significantly changed the tolerance of tobacco to B. cinerea. Taken together, the results demonstrated that sly‐miR1916 might regulate the expression of STR‐2, UGT, and MYB12 in tomato plant, conferring sensitivity to biotic stress via modulating α‐tomatine and anthocyanins.

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Prediction and validation of potential pathogenic microRNAs involved in Phytophthora infestans infection

Cited by 14 publications

References 34 publications

MiR1918 enhances tomato sensitivity to Phytophthora infestans infection

MiR1918 enhances tomato sensitivity to Phytophthora infestans infection

Sp-miR396a-5p acts as a stress-responsive genes regulator by conferring tolerance to abiotic stresses and susceptibility to Phytophthora nicotianae infection in transgenic tobacco

Solanum lycopersicum microRNA1916 targets multiple target genes and negatively regulates the immune response in tomato

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