Prediction of gradient retention data for hydrophilic interaction liquid chromatographic separation of native and fluorescently labeled oligosaccharides

Abstract

In this work, we have investigated the predictive properties of mixed-mode retention model and oligomeric mixed-mode model, taking into account the contribution of monomeric units to the retention, in hydrophilic interaction liquid chromatography. The gradient retention times of native maltooligosaccharides and their fluorescent derivatives were predicted in the oligomeric series with number of monomeric glucose units in the range from two to seven. The maltooligosaccharides were separated on a packed column with carbamoyl-bonded silica stationary phase and 15 gradient profiles with different initial and final mobile phase composition were used with the gradient times 5; 7.5 and 10min. The predicted gradient retention times were compared for calculations based on isocratic retention data and gradient retention data, which provided better accuracy of the results. By comparing two different mobile phase additives, the more accurate retention times were predicted in mobile phases containing ammonium acetate. The acidic derivatives, prepared by reaction of an oligosaccharide with 2-aminobenzoic acid or 8-aminonaphthalene-1,3,6-trisulfonic acid, provided more accurate predictions of the retention data in comparison to native oligosaccharides or their neutral derivatives. The oligomeric mixed-mode model allowed prediction of gradient retention times using only one gradient profile, which significantly speeded-up the method development.

Understanding and manipulating the separation in hydrophilic interaction liquid chromatography

“…They investigated the addition of a further term to the mixed mode model describing the contribution of the oligomeric glucose unit to retention. A later paper studied the application of the mixed mode equation and its extended variant to give predictions of the gradient retention time of the same solutes [79] .…”

Liquid chromatography at the University of Pardubice: A tribute to Professor Pavel Jandera

“…Inspired by the work of Professor Jandera, the prediction of gradient retention data for HILIC separation was studied by Česla et al [105]. Due to the application of the oligomeric mixed-mode model, Equation (9) cannot be solved analytically, and numerical integration was employed.…”