Prediction of Pasteurella multocida serotypes based on whole genomic sequences

Sajid, Sajid Mahmood; Bisgaard, Magne; Magistrali, Chiara Francesca; Massacci, Francesca Romana; Liman, Martin; Menke, Theresa; Bischoff, Henning; Olsen, John Elmerdahl

doi:10.1016/j.vetmic.2022.109492

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Cited by 5 publications

(2 citation statements)

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“…The whole-genome sequences were queried in the Pasteurella multocida Bacterial Isolate Genome Sequence Database ( https://ivsmlst.sund.ku.dk ), which typed both strains as capsular type D and lipopolysaccharide (LPS) group 6 ( 13 ).…”

Section: Announcementmentioning

confidence: 99%

De Novo Whole-Genome Sequencing of Two Pathogenic Pasteurella multocida Type D:6 Strains Isolated from Pigs

Livingstone

Jørgensen

McCall

et al. 2023

Microbiol Resour Announc

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Section: Announcementmentioning

confidence: 99%

De Novo Whole-Genome Sequencing of Two Pathogenic Pasteurella multocida Type D:6 Strains Isolated from Pigs

Livingstone

Jørgensen

McCall

et al. 2023

Microbiol Resour Announc

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“…Pasteurella multocida (P. multocida) is a facultative anaerobic Gram-negative bacillus that has five serogroups, A, B, D, E, and F, according to differences in its capsular antigens [1,2]. Its most important virulence factors are the capsule, outer membrane protein, lipopolysaccharide, P. multocida toxin (PMT), fimbriae, and adhesin molecules [3,4].…”

Section: Introductionmentioning

confidence: 99%

CXCL8 Knockout: A Key to Resisting Pasteurella multocida Toxin-Induced Cytotoxicity

Yuan,

Zhao,

et al. 2024

IJMS

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Pasteurella multocida, a zoonotic pathogen that produces a 146-kDa modular toxin (PMT), causes progressive atrophic rhinitis with severe turbinate bone degradation in pigs. However, its mechanism of cytotoxicity remains unclear. In this study, we expressed PMT, purified it in a prokaryotic expression system, and found that it killed PK15 cells. The host factor CXCL8 was significantly upregulated among the differentially expressed genes in a transcriptome sequencing analysis and qPCR verification. We constructed a CXCL8-knockout cell line with a CRISPR/Cas9 system and found that CXCL8 knockout significantly increased resistance to PMT-induced cell apoptosis. CXCL8 knockout impaired the cleavage efficiency of apoptosis-related proteins, including Caspase3, Caspase8, and PARP1, as demonstrated with Western blot. In conclusion, these findings establish that CXCL8 facilitates PMT-induced PK15 cell death, which involves apoptotic pathways; this observation documents that CXCL8 plays a key role in PMT-induced PK15 cell death.

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Development of a MALDI-TOF MS model for differentiating haemorrhagic septicaemia-causing strains of Pasteurella multocida from other capsular groups

Maddock,

Stenger,

Roberts

et al. 2024

Journal of Microbiological Methods

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Prediction of Pasteurella multocida serotypes based on whole genomic sequences

Cited by 5 publications

References 38 publications

De Novo Whole-Genome Sequencing of Two Pathogenic Pasteurella multocida Type D:6 Strains Isolated from Pigs

De Novo Whole-Genome Sequencing of Two Pathogenic Pasteurella multocida Type D:6 Strains Isolated from Pigs

CXCL8 Knockout: A Key to Resisting Pasteurella multocida Toxin-Induced Cytotoxicity

Development of a MALDI-TOF MS model for differentiating haemorrhagic septicaemia-causing strains of Pasteurella multocida from other capsular groups

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