Predictive Capacity of Boar Sperm Morphometry and Morphometric Sub-Populations on Reproductive Success after Artificial Insemination

Barquero, Vinicio; Roldán, Eduardo R. S.; Soler, Carles; Yániz, J.L.; Camacho, Marlen; Valverde, Anthony

doi:10.3390/ani11040920

Cited by 17 publications

(25 citation statements)

References 69 publications

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“…General length of spermatozoids has positive correlation relationship with speed and type of their movement. Spermatozoids with longer flagella are considered to move faster through sexual pathways of female and be the first to reach oviducts (Gorski et al, 2018;Barquero et al, 2021). During our studies, we determined that the general length of spermatozoids was the highest in sire boars which had been consuming the mineral additive for 45 days in optimum amount, which likely increased their fertilizing ability.…”

Section: Discussionmentioning

confidence: 97%

Effects of copper citrate on physiological-biochemical parameters of ejaculate of sire boars

Shostya

Siabro

2022

Regul. Mech. Biosyst.

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Revealing peculiarities of the course of physiological and biochemical processes in the formation of parameters of ejaculate allows effective correlation of biological completeness of spermatozoids based on the use of chelate compounds of limiting microelements, particularly copper. The study was focused on determining changes in processes of spermatogenesis during correction of mineral nutrition. In the experiment, we used adult boars of the same age, live weight and quality of ejaculates. They consumed copper citrate during the 45 day experiment in doses higher than the norm by 10% (1.5 mg per 1 kg of combined feed) and 20% (3 mg per 1 kg of combined feed) compared with the control. We determined difference in the effects of different doses of copper citrate on the formation of parameters of ejaculates in sire boars. Addition of the mineral additive to the feed in the doses 10% exceeding the norm promoted increase in the parameters of functional activity of spermatozoids – mobility and survival – on day 45 of the intake, which occurred against the background of acceleration of peroxidation processes (increase in the concentration of thiobarbituric acid of active compounds) and activation of the system of antioxidant protection – increase in the activity of superoxide dismutase and decrease in the level of catalase in the sperm. At the same time, we observed increase in the morphometric parameters of spermatozoids – general length, width, length and volume of the head. On day 30, sire boars that had been consuming copper citrate in the amounts 20% above the norm were observed to have increase in concentration of spermatozoids, their mobility and survivability, though a decrease was seen in the morphometric parameters of the gametes. Further intake of this element caused increase in the amount of abnormal forms of spermatozoids. Under the action of this additive, we determined increase in the amount of metabolites of lipid peroxidation – conjugated dienes and thiobarbituric acids of active compounds, and also weakening of the system of antioxidative protection – decrease in the content of reduced glutathione and accumulation of dehydroascorbic acid. Thus, the intake of copper citrate in the amounts of 10% above the norm facilitated morpho-functional parameters of spermatozoids by activating the system of antioxidant protection. It would be promising to conduct further research to determine the effects of copper citrate on the processes of activation and capacitation of spermatozoids and fertilization of oocytes in in vivo and in vitro conditions.

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Section: Discussionmentioning

confidence: 97%

Effects of copper citrate on physiological-biochemical parameters of ejaculate of sire boars

Shostya

Siabro

2022

Regul. Mech. Biosyst.

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“…The kinematic parameters measured were: curvilinear velocity (VCL), linear velocity (VSL), average path velocity (VAP), linearity (LIN), straightness (STR), wobble (WOB), lateral head displacement (ALH) and beat cross‐ frequency (BCF). The parameters defining progressive motility were STR ≥70% and VAP ≥25 μm/s, as previously described by Barquero et al (2021).…”

Section: Methodsmentioning

confidence: 99%

“…Morphometric parameters were assessed using the Diff Quik Kit (Medion Diagnostics, Germany) as previously described by Barquero et al (2021). For this analysis, 6557 sperm (200 cells/sample) images were captured at 400× magnification.…”

Section: Methodsmentioning

confidence: 99%

Sperm selection techniques in cattle: Microfilter device versus conventional methods

et al. 2022

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Microfluidics and microfilter devices have been developed to mimic the characteristics of the female reproductive tract, minimizing the risk of sperm damage. This study aimed to compare the use of a microfilter device versus conventional methods for sperm selection used in in vitro fertilization (IVF). For selecting spermatozoa, the pooled samples were processed in a microfilter device, swim-up and mini-Percoll gradient. Kinematic and morphometric parameters, vitality and DNA damage were analysed before and after sperm selection. After selection, 10,000 motile spermatozoa per oocyte were used in IVF drops. Embryos were assessed at three (cleavage rate) and seven (blastocyst rate) days post-IVF. Results of sperm kinematic parameters including average path velocity, velocity straight line, curvilinear velocity, linearity, lateral head displacement with the microfilter device were superior to density gradient (p < 0.05), but similar to swim-up method. Likewise, sperm DNA damage was significantly reduced using the microfilter device and swim-up method. Regarding the total sperm recovery rate post selection, results with the microfilter device (17.64%) and mini-Percoll gradient (18.27%) were higher than with swim-up method (6.52%).However, the cleavage and blastocyst rates were the lowest using the microfilter device. In conclusion, sperm selection using the microfilter device and swim-up method can improve kinematic parameters, although the mini Percoll gradient was the most efficient method for embryo production.

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“…Esto se ha analizado también con el uso de técnicas de análisis bayesiano, lo que ha producido resultados interesantes, pero todavía por explorar en mayor medida (Víquez et al, 2020). Aún se desconoce la funcionalidad relativa de cada una de estas subpoblaciones, pero ya hay indicios de su relación con la capacidad fecundante del eyaculado (Barquero, Roldan, Soler, Vargas-Leitón, et al, 2021;Barquero, Roldan, Soler, Yániz, et al, 2021;.…”

Section: Hacia Un Nuevo Paradigma De Qué Es El Eyaculadounclassified

El análisis seminal en la agricultura de precisión en el siglo XXI

Soler

Valverde

2023

Agron. Mesoam.

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Introducción. La reproducción asistida de animales tiene su origen en la domesticación de las especies ganaderas útiles al ser humano. Los consiguientes procesos de selección artificial permitieron desarrollar técnicas biotecnológicas que contribuyeron a la mejora de las capacidades de producción animal. Objetivo. Contextualizar el análisis seminal en la agricultura de precisión en el siglo XXI. Desarrollo. La visualización de espermatozoides en el microscopio puede considerarse como el primer paso en el advenimiento de la biotecnología de la reproducción y la base para el desarrollo de las técnicas de reproducción asistida. Con el perfeccionamiento de los métodos de microscopía, se logró caracterizar los gametos masculinos, lo que significó un avance significativo en la tecnología de inseminación artificial. El punto de inflexión marcado por el desarrollo de técnicas de conservación espermática, implicó un cambio sustantivo en el desarrollo de estas tecnologías en las especies ganaderas, ya sea con semen crio preservado o refrigerado. Estos métodos son de alto valor en el caso de especies amenazadas, dado que se pueden crear bancos de germoplasma con propósitos de conservación genética y rescate de especies en riesgo de extinción. El análisis seminal se ha desarrollado de forma paralela con las técnicas de reproducción asistida, al punto que hoy se considera como una técnica relevante en la biotecnología de la reproducción animal, que se ha perfeccionado mediante el avance de la ciencia y la tecnología, la física óptica y la computación. Conclusión. El análisis seminal ha tenido un cambio de paradigma al desechar técnicas obsoletas de evaluación subjetiva de la calidad seminal y adoptar métodos objetivos de evaluación del semen, mediante el análisis de grandes volúmenes de datos y variables de movilidad, cinéticas, morfométricas, morfológicas y fragmentación del ADN, que permiten caracterizar mejor los eyaculados de los reproductores en centros de inseminación artificial.

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Predictive Capacity of Boar Sperm Morphometry and Morphometric Sub-Populations on Reproductive Success after Artificial Insemination

Cited by 17 publications

References 69 publications

Effects of copper citrate on physiological-biochemical parameters of ejaculate of sire boars

Effects of copper citrate on physiological-biochemical parameters of ejaculate of sire boars

Sperm selection techniques in cattle: Microfilter device versus conventional methods

El análisis seminal en la agricultura de precisión en el siglo XXI

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