Predictive early gene signature during mouse Bhas 42 cell transformation induced by synthetic amorphous silica nanoparticles

Kirsch, Anaïs; Dubois-Pot-Schneider, Hélène; Fontana, C. M.; Schohn, Hervé; Gaté, Laurent; Guichard, Yves

doi:10.1016/j.cbi.2019.108900

Cited by 4 publications

(3 citation statements)

References 58 publications

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“…A concentration-dependent increase in cell transformation ability was demonstrated in Balb/c 3T3 [41] and Caco-2 cells [43], as well as induced malignancies in BEAS-2B cells exposed to Ag-NP [42]. Synthetic amorphous silica nanoparticle (SAS) (NM-200, nm-201, nm-202, and nm-203)-exposed Bhas 42 cell transformation model showed concentration dependency [45,46]. Only the highest concentration (20 µg/mL) showed significant cell transformation ability in TiO 2 -NP (NM102)-exposed BEAS-2B cells [54].…”

Section: Exposure Concentrationsmentioning

confidence: 99%

“…Size-dependent cell transformation was observed when the HBEC-3KT cell line was exposed to the TiO 2 -NP and MWCNT with a smaller diameter but not to that with a larger diameter, in the soft-agar colony formation assay [57]. SAS (NM-200 and nm-201, nm-202 and nm-203) were reported as tumor-promoter substances in the Bhas 42 cell transformation model, and a marked size-dependency was apparent, in which a higher the particle size indicated a higher transformation ability [45]. In a comparison between coated (either with citrate or silica) vs. uncoated TiO 2 -NP and ZrO 2 NP, it was shown that coating with silica seems to prevent Balb/3T3 morphological transformation induced by ZrO 2 NP [58].…”

Section: The Influence Of Physicochemical Properties Of Nms In Cell T...mentioning

confidence: 99%

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In Vitro Cell Transformation Assays: A Valuable Approach for Carcinogenic Potentiality Assessment of Nanomaterials

Chatterjee

Alfaro-Moreno

2023

IJMS

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This review explores the application of in vitro cell transformation assays (CTAs) as a screening platform to assess the carcinogenic potential of nanomaterials (NMs) resulting from continuously growing industrial production and use. The widespread application of NMs in various fields has raised concerns about their potential adverse effects, necessitating safety evaluations, particularly in long-term continuous exposure scenarios. CTAs present a realistic screening platform for known and emerging NMs by examining their resemblance to the hallmark of malignancy, including high proliferation rates, loss of contact inhibition, the gain of anchorage-independent growth, cellular invasion, dysregulation of the cell cycle, apoptosis resistance, and ability to form tumors in experimental animals. Through the deliberate transformation of cells via chronic NM exposure, researchers can investigate the tumorigenic properties of NMs and the underlying mechanisms of cancer development. This article examines NM-induced cell transformation studies, focusing on identifying existing knowledge gaps. Specifically, it explores the physicochemical properties of NMs, experimental models, assays, dose and time requirements for cell transformation, and the underlying mechanisms of malignancy. Our review aims to advance understanding in this field and identify areas for further investigation.

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Section: Exposure Concentrationsmentioning

confidence: 99%

Section: The Influence Of Physicochemical Properties Of Nms In Cell T...mentioning

confidence: 99%

In Vitro Cell Transformation Assays: A Valuable Approach for Carcinogenic Potentiality Assessment of Nanomaterials

Chatterjee

Alfaro-Moreno

2023

IJMS

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“…Recently, in vitro and in vivo models were utilized to demonstrate that silica NPs in Xuanwei bituminous coal may play an important role in the development of lung cancer by inducing inflammation (Li et al, 2019). More recently, it was found that genes related to the early stage of cell transformation induced by silica NPs are related to cancer progression (Kirsch et al, 2020).…”

Section: Nano‐metal Oxidesmentioning

confidence: 99%

Research progress on the carcinogenicity of metal nanomaterials

Liu

Kong

2021

J of Applied Toxicology

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With the rapid development of nanotechnology, new nanomaterials with enormous potentials continue to emerge, especially metal nanomaterials. Metal nanomaterials possess the characteristics of metals and nanomaterials, so they are widely used in many fields. But at the same time, whether the use or release of metal nan4omaterials into the environment is toxic to human beings and animals has now attained widespread attention at home and abroad. Currently, it is an indisputable fact that cancer ranks among the top causes of death among residents worldwide. The properties of causing DNA damage and mutations possessed by these metal nanomaterials make them unpredictable influences in the body, subsequently leading to genotoxicity and carcinogenicity. Due to the increasing evidence of their roles in carcinogenicity, this article reviews the toxicological and carcinogenic effects of metal nanomaterials, including nano‐metal elements (nickel nanoparticles, silver nanoparticles, and cobalt nanoparticles) and nano‐metal oxides (titanium dioxide nanoparticles, silica nanoparticles, zinc oxide nanoparticles, and alumina nanoparticles). This article provides a reference for the researchers and policymakers to use metal nanomaterials rationally in modern industries and biomedicine.

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In vitro cell-transforming potential of secondary polyethylene terephthalate and polylactic acid nanoplastics

Domenech,

Villacorta,

Ferrer

et al. 2024

Journal of Hazardous Materials

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Predictive early gene signature during mouse Bhas 42 cell transformation induced by synthetic amorphous silica nanoparticles

Cited by 4 publications

References 58 publications

In Vitro Cell Transformation Assays: A Valuable Approach for Carcinogenic Potentiality Assessment of Nanomaterials

In Vitro Cell Transformation Assays: A Valuable Approach for Carcinogenic Potentiality Assessment of Nanomaterials

Research progress on the carcinogenicity of metal nanomaterials

In vitro cell-transforming potential of secondary polyethylene terephthalate and polylactic acid nanoplastics

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