Predominance of G10 Genotype of Rotavirus in Diarrheic Buffalo Calves: A Potential Threat for Animal to Human Zoonotic Transmission

Abstract

| Gastroenteritis among young dairy calves is predominantly caused by group A rotaviruses (RVA), which leads to calf mortality and significant economic losses to dairy farmers. Huge genetic and antigenic diversity exists amongst different RVA isolates due to segmented nature of the dsRNA genome and diverse host range. The RNA-PAGE analysis of 11 diarrheic fecal samples of buffalo calves from organized dairy farm in Hisar region showed presence of RVA. The VP7 (G type) and VP4 (P type) gene based genotyping of all the sample was carried out by semi nested multiplex RT-PCR. All the samples yielded expected product of 864 bp for VP4 and 1013 bp for VP7 genes, respectively, using generic primers. Nine of 11 (81.81%) samples were categorized as P[11] exhibiting 332 bp product whilst two samples remained un-typed by semi-nested multiplex PCR using cocktail of genotype specific primers for P[1], P[5] and P[11] targeting VP4 gene. The G genotyping of VP7 genes yielded 644 bp products in 8 (72.72%) and were categorized as G10. The remaining 3 (27.27%) samples yielded 288 bp products specific for G6 genotypes. The P genotype for two of the G6 genotypes remained unknown by semi nested multiplex RT-PCR. The 8 (72.72%) isolates with G10 genotype were classified to bear G10 P[11], where, as only one G6 genotyped sample was determined to have G6 P[11] genotype combination. Recently, increasing incidences of involvement of animal rotavirus with G10 specificity in human's gastroenteritis poses a serious threat of zoonotic transmission of this virus. Hence presence of G10 type in humans is of great concern and widespread surveillance for animal and human rotaviruses is needed to determine the diversity of rotaviruses circulating in various parts of India before adopting any immunization programs.

Group A Rotavirus Genotypes among Calf Herds in Southwest Nigeria and Implications for Human Rotavirus Vaccines’ Efficiency

“…tified. The zoonotic potential of G10 genotype of bovine strain was evidenced by its transmission from cattle to humans and vice-versa as previously reported and further corroborated by the detection of the G10 strain from buffalo calves and much recently from this study in Nigeria[14] [21][22]. The presence of rotavirus in many species of domestic animals results in serious epidemiological problems whose significance is becoming evident by the detection of genotypes of the virus believed to be strictly of animal origin in human subjects and vice-versa[23].…”

Molecular characterization of unusual G10P[33], G6P[14] genomic constellations of group A rotavirus and evidence of zooanthroponosis in bovines

“…In the present study, out of samples analyzed from diarrheic calves, 27 Evidences from epidemiological studies worldwide indicates, G6 and G10 along with P [5] and P [11] are the most common genotypes identified in bovines. The predominance of G10P [11] combination observed in this study is in concurrence with the previous reports from India (Beg et al, 2010;Minakshi et al, 2015, Tatte et al, 2019. However, these findings are in contrast with Indian studies with G3P [11] predominance (Malik et al, 2012;Sravani et al, 2014) and reports from Iran (Pourasgari et al, 2016) and Ireland with G6P [5] as major genotype (Collins et al, 2014).…”

Molecular characterization of unusual G10P[33], G6P[14] genomic constellations and evidence of zooanthroponosis in bovines

“…Evidences from epidemiological studies worldwide indicates, G6 and G10 along with P[5] and P[11] are the most common genotypes identified in bovines. The predominance of G10P[11] combination observed in this study is in concurrence with the previous reports from India (Beg et al, 2010; Minakshi et al, 2015, Tatte et al, 2019). However, these findings are in contrast with Indian studies with G3P[11] predominance (Malik et al, 2012; Sravani et al, 2014) and reports from Iran (Pourasgari et al ., 2016) and Ireland with G6P[5] as major genotype (Collins et al ., 2014).…”