Preformulados para control de la mosca blanca Bemisia tabaci (Hemiptera: Aleyrodidae) en condiciones de laboratorio

Abstract

Bemisia tabaci es una plaga de importancia económica debido a su amplia distribución geográfica, al daño que ocasiona, y al gran número de cultivos que afecta. En Colombia el control biológico mediante el uso de bioplaguicidas surge como una alternativa promisoria para programas de manejo integrado de plagas. Por esta razón, el objetivo del presente trabajo fue desarrollar y evaluar preformulados con base en hongos entomopatógenos nativos contra B. tabaci. Se evaluaron diferentes sustratos para la producción masiva de los aislamientos Bv 056 de Beauveria bassiana, Pc 013 de Paecilomyces sp. y Vl 026 de Lecanicillium lecanii. Se seleccionó como el de mayor rendimiento y fácil manipulación un sustrato sólido con base en cereales que presentó un rendimiento promedio de 1x109 conidios/g. El principio activo del bioplaguicida consistente en los conidios separados del medio de cultivo fue caracterizado microbiológica y físicamente. Se evaluó bajo condiciones de laboratorio el efecto de los preformulados sobre ninfas de segundo instar, infestando foliolos de fríjol y aplicándolos a una concentración de 1x108 conidios/mL. Se produjo el mayor porcentaje de eficacia con el preformulado a base de B. bassiana con un 96,5% a los 14 días postaplicación, seguido por los preformulados de Paecilomyces sp. y L. lecanii, con 81,8% y de 70%, respectivamente.

In vitro versus in planta: Comparing the compatibility of Akanthomyces lecanii with pesticides against Trialeurodes vaporariorum

“…Fourteen days after application, the infested leaves were collected. The number of individuals that completed the nymphal stage was determined, as evidenced by the T‐shaped opening exuviae or nymphs with signs of infection, characterized by their pale yellowish colour and flattened appearance (Espinel et al, 2008). Mortality was determined by subtracting the final live nymphs count from those initially counted.…”

“…The experiment was set up under greenhouse conditions located at 'Tibaitatá' Research Center of Agrosavia (Mosquera, Colombia) following the methodology described by Espinel et al (2008) with minor modifications. Bean seed was sown in pots.…”

“…The artificial infestation was carried out with 30 adults per leaflet arranged in clamp cages (120 adults per treatment), which were removed after 48 h. The initial nymph count (second instar nymphs) was carried out 10 days later, and immediately after, the treatments were applied by an airbrush, with a volume of 600 μl, at a concentration of 1 × 10 7 conidia per ml. (Espinel et al, 2008). Mortality was determined by subtracting the final live nymphs count from those initially counted.…”

“…Lecabiol WG® is a Colombian registered biopesticide [Active ingredient: Akanthomyces lecanii (Zimm.) Spatafora, Kepler & B. Shrestha, 2017 (Hypocreales: Cordycipitaceae)] for whiteflies control ( Bemisia tabaci and T. vaporariorum ) in cotton, soybean, bean, eggplant, golden berry and tomato (Espinel et al, 2008; Santos et al, 2018). Considering that, this bioproduct is applied with several pesticides (primarily insecticides and fungicides) to reduce the damage caused by different pests and fungal diseases; therefore, compatibility testing is required to ensure the viability, persistence and efficacy of the entomopathogen against the whitefly (Khun et al, 2020; Wari et al, 2020).…”

Differential Efficacies of Nomuraea Rileyi and Isaria Fumosorosea on Some Serious Pests and the Pests’ Efficient Predator Prevailing in Tomato Fields in Egypt

“…Contrary to the other specific microbial insecticides, entomopathogenic fungi have successfully controlled a wide range of insect pests (Sabbour and Shadia Abd El-Aziz 2002;Sahab 2005, 2007;Thungrabeab and Tongma 2007;Sahab and Sabbour 2011). The two microbial control agents, Nomuraea rileyi and Isaria fumosorosea, proved highly pathogenic to aphids and whiteflies (Espinel et al 2008). The fungus N. rileyi, exhibits host preferential infections in lepidopterous larvae (Ignoffo et al 1976).…”