Pregnancy after ICSI with ejaculated immotile spermatozoa from a patient with immotile cilia syndrome: a case report and review of the literature

Peeraer, Karen; Nijs, Martine; Raick, Dominique; Ombelet, Willem

doi:10.1016/s1472-6483(10)61777-4

Cited by 49 publications

(29 citation statements)

References 25 publications

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“…In another case where the HOS test was used for selection of spermatozoa from a husband with ICS, two grade A embryos at 7-and 8-cells were transferred on day three and resulted in a pregnancy that miscarried at 21 weeks. The subsequent FET resulted in the transfer of two grade A morulae that resulted in a single intrauterine pregnancy [17].…”

Section: Discussionmentioning

confidence: 99%

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Successful twin birth following blastocyst culture of embryos derived from the immotile ejaculated spermatozoa from a patient with primary ciliary dyskinesia: A case report

Kordus

Price

Davis

et al. 2008

J Assist Reprod Genet

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Purpose To describe the ultrastructure of spermatozoa from a patient with complete asthenozoospermia that resulted in live births following blastocyst culture. Materials and methods Analyses of spermatozoa from a 36 year old patient were performed using light and electron microscopy. The hypo-osmotic swelling test was used to select spermatozoa for intracytoplasmic sperm injection. Embryos were cultured to the blastocyst stage. Results 100% of the spermatozoa had dynein arm deficiency with secondary defects varying from 3-17%. Six oocytes were injected; five fertilized normally and one was digynic. All five zygotes formed good quality blastocysts. Three blastocysts were cryopreserved and two blastocysts were transferred. Twin females were born at 37 weeks. Conclusions The hypo-osmotic swelling test can be used to select viable immotile ejaculated spermatozoa from a patient with dynein arm deficiency and can produce excellent fertilization rates and blastocyst development resulting in live births.

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Section: Discussionmentioning

confidence: 99%

“…The hypo-osmotic swelling (HOS) test [14] was used in conjunction with ICSI of immotile spermatozoa with success in several cases as well [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Successful twin birth following blastocyst culture of embryos derived from the immotile ejaculated spermatozoa from a patient with primary ciliary dyskinesia: A case report

Kordus

Price

Davis

et al. 2008

J Assist Reprod Genet

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“…With respect to this, HOS test was found to be helpful since several authors reported pregnancies after selection based on osmotic behavior of the sperm membrane which allows estimating sperm viability [9,11,16].…”

Section: Discussionmentioning

confidence: 99%

“…In patients suffering from PCD or Kartagener syndrome, however, male infertility derives from ultrastructural defects of the flagella, e.g., absence of the inner dynein arms. This scenario automatically leads to severe asthenozoospermia [4][5][6] or much rather to complete absence of motile gametes [7][8][9][10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Healthy twin live-birth after ionophore treatment in a case of theophylline-resistant Kartagener syndrome

Ebner

Maurer

Oppelt

et al. 2015

J Assist Reprod Genet

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Purpose To evaluate whether it is a feasible option to target the oocyte (with Ca 2+ -ionophore) in case that sperm motility cannot be restored in Kartagener syndrome. Methods A case of a male Kartagener syndrome with exclusively immotile spermatozoa that did not react to the dimethylxanthine theophylline. Thus, half of the associated oocytes were treated for 15 min with the ready-to-use-ionophore CultActive immediately after ICSI whereas the other 50 % were injected with routine ICSI without artificial oocyte activation. Rates of fertilization, blastulation, pregnancy and live birth were evaluated. Results Fertilization check revealed that none of the conventionally injected but 4/6 (66.7 %) of the artificially activated oocytes showed two pronuclei. Three embryos were of good and one of fair quality. Corresponding blastocyst formation rate was 3 out of 4 (75 %). A double embryo transfer led to a healthy twin birth in the 34th week of gestation (two boys with a birth weight of 1724 g and 2199 g). Conclusions This case indicates that Ca 2+-ionophore treatment in cycles from theophylline-resistant Kartagener syndrome patients is a feasible option. The future will show if routine application of A23187 in Kartagener or primary cilia dyskinesis patients will be of benefit.

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“…The HOS test is indicated in cases when there are very few or no motile sperm present in the ejaculate. Dead spermatozoa whose 80 As of today, the HOS test is generally used as additional indicator of sperm vitality and can be utilized in cases diagnosed with immotile cilia syndrome 81 or severe astenozoospermia.…”

Section: Hypo-osmotic Swelling (Hos) Testmentioning

confidence: 99%

Semen analysis and sperm function testing

Franken¹,

Oehninger²

2011

Asian J Androl

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Despite controversy regarding the clinical value of semen analysis, male fertility investigation still relies on a standardized analysis of the semen parameters. This is especially true for infertility clinics in both developing and developed countries. Other optional tests or sophisticated technologies have not been widely applied. The current review addresses important changes in the analysis of semen as described in the new World Health Organization (WHO) manual for semen analysis. The most important change in the manual is the use of evidence-based publications as references to determine cutoff values for normality. Apart from the above mentioned changes, the initial evaluation and handling methods remain, in most instances, the same as in previous editions. Furthermore, the review evaluates the importance of quality control in andrology with emphasis on the evaluation of sperm morphology. WHO sperm morphology training programmes for Sub-Saharan countries were initiated at Tygerberg Hospital in 1995. The external quality control programme has ensured that the majority of participants have maintained their morphological reading skills acquired during initial training. This review reports on current sperm functional tests, such as the induced acrosome reaction, and sperm-zona pellucida binding assays, as well as the impact of sperm quality in terms of DNA integrity, and the relationship of sperm function tests to sperm morphology.

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Pregnancy after ICSI with ejaculated immotile spermatozoa from a patient with immotile cilia syndrome: a case report and review of the literature

Cited by 49 publications

References 25 publications

Successful twin birth following blastocyst culture of embryos derived from the immotile ejaculated spermatozoa from a patient with primary ciliary dyskinesia: A case report

Successful twin birth following blastocyst culture of embryos derived from the immotile ejaculated spermatozoa from a patient with primary ciliary dyskinesia: A case report

Healthy twin live-birth after ionophore treatment in a case of theophylline-resistant Kartagener syndrome

Semen analysis and sperm function testing

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