Preharvest UV-C affects lettuce resistance to Xanthomonas campestris pv. vitians and quality

Sidibé, Amadou; Charles, Marie Thérèse; Nicolas, Olbert; Beaulieu, Carole

doi:10.1016/j.scienta.2021.110094

Cited by 4 publications

(7 citation statements)

References 36 publications

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“…vitians -B07-007 ( Xcv ) ( Nicolas et al, 2018 ), was used in this study. Seeding was carried out in a 98-well tray in a germinator for 2 weeks, and seedlings were then transferred to growth chambers as described by Sidibé et al (2021) . The plants were watered daily, alternating between water and a nutrient solution containing 200 ppm nitrogen, 71 ppm potassium and 200 ppm phosphorus.…”

Section: Methodsmentioning

confidence: 99%

“…Ultraviolet-C treatments began 3 weeks after sowing, corresponding to the transplanting stage of lettuce in the field as described by Sidibé et al (2021) . The growth chambers (Conviron, PGV40, MB, Canada) were modified with a set of three UV-C lamps (254 nm, 160 W, Clean Light Inc., Vineland Station, ON, Canada).…”

Section: Methodsmentioning

confidence: 99%

“…Among the beneficial effects of UV-C on lettuce, Sidibé et al (2021) showed an increase in dry matter and resistance against Xcv with no negative impact on yield. The work of Sidibé et al (2021) suggests that the lettuce plant is able to integrate the negative effects of UV-C-induced oxidative stress signals, return to a state of homeostasis and pursue normal growth while mounting an effective defense fight against BLS. Based on these premises, using transcriptomics in the present study, we propose a hypothetical model describing the regulatory mechanisms that may underlie the beneficial effects induced by preharvest hormetic doses of UV-C radiation on lettuce.…”

Section: Introductionmentioning

confidence: 99%

“…Numerous studies have shown the beneficial effects of postharvest UV-C hormesis in several plant species (for a review, see Duarte-Sierra et al, 2019 ). When applied at the preharvest stage, the beneficial effects of UV-C hormesis have been shown in strawberry ( Janisiewicz et al, 2016a , b ; Xie et al, 2016 ; Xu et al, 2019 ), tomato ( Valencia et al, 2017 ), and lettuce ( Vàsquez et al, 2017 ; Sidibé et al, 2021 ). Among the beneficial effects of UV-C on lettuce, Sidibé et al (2021) showed an increase in dry matter and resistance against Xcv with no negative impact on yield.…”

Section: Introductionmentioning

confidence: 99%

“…When applied at the preharvest stage, the beneficial effects of UV-C hormesis have been shown in strawberry ( Janisiewicz et al, 2016a , b ; Xie et al, 2016 ; Xu et al, 2019 ), tomato ( Valencia et al, 2017 ), and lettuce ( Vàsquez et al, 2017 ; Sidibé et al, 2021 ). Among the beneficial effects of UV-C on lettuce, Sidibé et al (2021) showed an increase in dry matter and resistance against Xcv with no negative impact on yield. The work of Sidibé et al (2021) suggests that the lettuce plant is able to integrate the negative effects of UV-C-induced oxidative stress signals, return to a state of homeostasis and pursue normal growth while mounting an effective defense fight against BLS.…”

Section: Introductionmentioning

confidence: 99%

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Preharvest UV-C Hormesis Induces Key Genes Associated With Homeostasis, Growth and Defense in Lettuce Inoculated With Xanthomonas campestris pv. vitians

et al. 2022

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Preharvest application of hormetic doses of ultraviolet-C (UV-C) generates beneficial effects in plants. In this study, within 1 week, four UV-C treatments of 0.4 kJ/m2 were applied to 3-week-old lettuce seedlings. The leaves were inoculated with a virulent strain of Xanthomonas campestris pv. vitians (Xcv) 48 h after the last UV-C application. The extent of the disease was tracked over time and a transcriptomic analysis was performed on lettuce leaf samples. Samples of lettuce leaves, from both control and treated groups, were taken at two different times corresponding to T2, 48 h after the last UV-C treatment and T3, 24 h after inoculation (i.e., 72 h after the last UV-C treatment). A significant decrease in disease severity between the UV-C treated lettuce and the control was observed on days 4, 8, and 14 after pathogen inoculation. Data from the transcriptomic study revealed, that in response to the effect of UV-C alone and/or UV-C + Xcv, a total of 3828 genes were differentially regulated with fold change (|log2-FC|) > 1.5 and false discovery rate (FDR) < 0.05. Among these, of the 2270 genes of known function 1556 were upregulated and 714 were downregulated. A total of 10 candidate genes were verified by qPCR and were generally consistent with the transcriptomic results. The differentially expressed genes observed in lettuce under the conditions of the present study were associated with 14 different biological processes in the plant. These genes are involved in a series of metabolic pathways associated with the ability of lettuce treated with hormetic doses of UV-C to resume normal growth and to defend themselves against potential stressors. The results indicate that the hormetic dose of UV-C applied preharvest on lettuce in this study, can be considered as an eustress that does not interfere with the ability of the treated plants to carry on a set of key physiological processes namely: homeostasis, growth and defense.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%