Preliminary Classification of the ABC Transporter Family in Betula halophila and Expression Patterns in Response to Exogenous Phytohormones and Abiotic Stresses

An, Linjun; Ma, Qing; Du, Jinxia; Yu, Min; Li, Fangrui; Luan, Jiayu; Jiang, Jing; Li, Huiyu

doi:10.3390/f10090722

Cited by 12 publications

(10 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The Arabidopsis ABP, AtABCG25/WBC26 transporter, is an ABA efflux carrier, and its overexpression reduced the water loss by facilitating ABA delivery to guard cells 88 ; similarly, expression of AtABCG36 in transgenic Arabidopsis plants contributed to drought stress and salt resistance 89 . An increased level of ABP and GBP was observed in Arabidopsis and Betula halophila plants under drought stress, and the significant increase in ABP is consistent with the increased activity of ATPase 90 , 91 . Thus, all three date palm cultivars opted for similar molecular mechanisms to mitigate the drought stress by overexpressing the ABP-associated ESTs.…”

Section: Discussionsupporting

confidence: 63%

Common and novel metabolic pathways related ESTs were upregulated in three date palm cultivars to ameliorate drought stress

Alhajhoj

Munir

Sudhakar

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Date palm is an important staple crop in Saudi Arabia, and about 400 different date palm cultivars grown here, only 50–60 of them are used commercially. The most popular and commercially consumed cultivars of these are Khalas, Reziz, and Sheshi, which are also widely cultivated across the country. Date palm is high water-demanding crop in oasis agriculture, with an inherent ability to tolerate drought stress. However, the mechanisms by which it tolerates drought stress, especially at the transcriptomic level, are still elusive. This study appraised the physiological and molecular response of three commercial date palm cultivars Khalas, Reziz, and Sheshi at two different field capacities (FC; 100% and 25%) levels. At 25% FC (drought stress), leaf relative water content, chlorophyll, photosynthesis, stomatal conductance, and transpiration were significantly reduced. However, leaf intercellular CO2 concentration and water use efficiency increased under drought stress. In comparison to cvs. Khalas and Reziz, date palm cv. Sheshi showed less tolerance to drought stress. A total of 1118 drought-responsive expressed sequence tags (ESTs) were sequenced, 345 from Khalas, 391 from Reziz, and 382 from Sheshi and subjected to functional characterization, gene ontology classification, KEGG pathways elucidation, and enzyme codes dissemination. Three date palm cultivars deployed a multivariate approach to ameliorate drought stress by leveraging common and indigenous molecular, cellular, biological, structural, transcriptional and reproductive mechanisms. Approximately 50% of the annotated ESTs were related to photosynthesis regulation, photosynthetic structure, signal transduction, auxin biosynthesis, osmoregulation, stomatal conductance, protein synthesis/turnover, active transport of solutes, and cell structure modulation. Along with the annotated ESTs, ca. 45% of ESTs were novel. Conclusively, the study provides novel clues and opens the myriads of genetic resources to understand the fine-tuned drought amelioration mechanisms in date palm.

show abstract

Section: Discussionsupporting

confidence: 63%

Common and novel metabolic pathways related ESTs were upregulated in three date palm cultivars to ameliorate drought stress

Alhajhoj

Munir

Sudhakar

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Stress in plants creates hormonal imbalances, leading to distorted metabolic function and interfering with plant growth and development. In response to stress, plants often adjust various physiological functions to accommodate or compensate for the affected hormone and adapt to harsh environmental conditions ( An et al, 2019 ). A candidate gene, TraesCS6A01G353700 , located on chromosome 6A, codes for Chlorophyllide a oxygenase (CAO), which plays a critical role in stress-related responses.…”

Section: Discussionmentioning

confidence: 99%

Genomic Regions, Molecular Markers, and Flanking Genes of Metribuzin Tolerance in Wheat (Triticum aestivum L.)

et al. 2022

View full text Add to dashboard Cite

Understanding the genetics of metribuzin (a group C herbicide) tolerance in wheat is vital in developing tolerant cultivars to improve wheat productivity in dryland farming systems. This study investigated metribuzin tolerance in wheat by conducting a Genome-wide Association Studies (GWAS) with a panel of 150 wheat genotypes of diverse genetic backgrounds and genotyped them with the wheat 90 K SNP genotyping assay. The phenotyping was conducted in a temperature-controlled glasshouse at the University of Western Australia (UWA). Genotypes were sprayed with a metribuzin dose of 400 grams of active ingredient (g. a.i.) ha−1 as pre-emergent in a specialized spraying cabinet and transferred to the glasshouse where the tolerance level of the genotypes was assessed by measuring the relative reduction in chlorophyll content of the leaves. The decrease in chlorophyll content of the treated plants compared to the control was regarded as the phytotoxic effects of metribuzin. GWAS analysis following a mixed linear model revealed 19 genomic regions with significant marker-trait associations (MTAs), including ten on chromosome 6A, three on chromosome 2B, and one on chromosomes 3A, 5B, 6B 6D, 7A, and 7B, respectively. Sequences of the significant markers were blasted against the wheat genome, IWGSC RefSeq V1.0, and candidate genes having annotations related to herbicide tolerance in wheat, especially in pathways reported to be involved in metribuzin tolerance, such as cytochrome P450 pathways and ATP Binding Cassette (ABC) superfamilies, were identified in these genomic regions. These included TraesCS6A01G028800, TraesCS6A02G353700, TraesCS6A01G326200, TraesCS7A02G331000, and TraesCS2B01G465200. These genomic regions were validated on 30 top tolerant and 30 most susceptible genotypes using the five closest SSR makers to the flanked SNPs. Sufficient polymorphism was detected on two markers (wms193 and barc1036) that were found to differentiate between the susceptible and tolerant alleles and a t-test analysis of the phenotypic data shows a significant (value of p < 0.001) difference suggesting that these markers can be used for marker-assisted selection (MAS) in metribuzin studies and wheat breeding programs.

show abstract

“…Deppe et al (2018) identified the ABC transporter Lr34 from wheat and reported its potential role in manipulating the composition of phospholipids in the plasma membrane and triggering the biotic and abiotic stress response in barley. Similarly, An et al (2019) observed significant changes in the expression of genes belonging to the ABC transporter family (ABCB and ABCG subfamilies) in response to exogenous phytohormones treatment and abiotic stresses. Their gene expression was reported to be linked with ABA‐dependent signaling pathways, which play an important role in modulating the stress tolerance level.…”

Section: Functions Of Abc Transporters In Heat Stress Tolerance Of Plantsmentioning

confidence: 91%

“…ABC transporters have been characterized in other plant models. For example, in Betula halophile , a model plant that survives under extreme salinity and drought conditions, has 15 diverse ABC transporter genes (An et al, 2019). The identified ABC transporters were classified into four sub‐families: ABCB, ABCG, ABCF and ABCI.…”

Section: Classification Of Abc Transporters and Their Structuresmentioning

confidence: 99%

“…Betula halophila , a member of the Betulaceae family, is highly tolerant to salt and drought stresses and has been used as model plant to understand the role of ABC transporters in salt tolerance. Further, using advanced techniques, 15 different BhABC genes were identified and validated to be involved in modulating the tolerance against salt and drought stresses (An et al, 2019). ABCB and ABCG were reported to be the most active in responses to hormones and abiotic stress.…”

Section: Abc Transporter‐mediated Regulation Of Salinity Stressmentioning

confidence: 99%

See 1 more Smart Citation

Role of ATP‐binding cassette transporters in maintaining plant homeostasis under abiotic and biotic stresses

Dahuja

Kumar

Sakhare

et al. 2020

Physiologia Plantarum

128

View full text Add to dashboard Cite

The ATP‐binding cassette (ABC) transporters belong to a large protein family predominantly present in diverse species. ABC transporters are driven by ATP hydrolysis and can act as exporters as well as importers. These proteins are localized in the membranes of chloroplasts, mitochondria, peroxisomes and vacuoles. ABC proteins are involved in regulating diverse biological processes in plants, such as growth, development, uptake of nutrients, tolerance to biotic and abiotic stresses, tolerance to metal toxicity, stomatal closure, shape and size of grains, protection of pollens, transport of phytohormones, etc. In mitochondria and chloroplast, the iron metabolism and its transport across the membrane are mediated by ABC transporters. Tonoplast‐localized ABC transporters are involved in internal detoxification of metal ion; thus protecting against the DNA impairment and maintaining cell growth. ABC transporters are involved in the transport of secondary metabolites inside the cells. Microorganisms also engage a large number of ABC transporters to import and expel substrates decisive for their pathogenesis. ABC transporters also suppress the seed embryonic growth until favorable conditions come. This review aims at giving insights on ABC transporters, their evolution, structure, functions and roles in different biological processes for helping the terrestrial plants to survive under adverse environmental conditions. These specialized plant membrane transporters ensure a sustainable economic yield and high‐quality products, especially under unfavorable conditions of growth. These transporters can be suitably manipulated to develop ‘Plants for the Future’.

show abstract

Preliminary Classification of the ABC Transporter Family in Betula halophila and Expression Patterns in Response to Exogenous Phytohormones and Abiotic Stresses

Cited by 12 publications

References 35 publications

Common and novel metabolic pathways related ESTs were upregulated in three date palm cultivars to ameliorate drought stress

Common and novel metabolic pathways related ESTs were upregulated in three date palm cultivars to ameliorate drought stress

Genomic Regions, Molecular Markers, and Flanking Genes of Metribuzin Tolerance in Wheat (Triticum aestivum L.)

Role of ATP‐binding cassette transporters in maintaining plant homeostasis under abiotic and biotic stresses

Contact Info

Product

Resources

About