Preliminary clinical report on safety and efficacy of photodynamic therapy using talaporfin sodium for malignant gliomas

Akimoto, Jiro; Haraoka, Jo; Aizawa, Katsuo

doi:10.1016/j.pdpdt.2012.01.001

Cited by 114 publications

(82 citation statements)

References 17 publications

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“…NPe6 has proven selective vascular effects in preclinical studies, an acceptable photosensitivity period of five to seven days, and a positive safety profile [94–97]. We selected a light source to target a secondary absorption peak of 664nm [98].…”

Section: Development Of New Treatment Approachesmentioning

confidence: 99%

The Role of Laser Speckle Imaging in Port-Wine Stain Research: Recent Advances and Opportunities

Choi

Tan

Jia

et al. 2016

IEEE J. Select. Topics Quantum Electron.

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Here, we review our current knowledge on the etiology and treatment of port-wine stain (PWS) birthmarks. Current treatment options have significant limitations in terms of efficacy. With the combination of 1) a suitable preclinical microvascular model, 2) laser speckle imaging (LSI) to evaluate blood-flow dynamics, and 3) a longitudinal experimental design, rapid preclinical assessment of new phototherapies can be translated from the lab to the clinic. The combination of photodynamic therapy (PDT) and pulsed-dye laser (PDL) irradiation achieves a synergistic effect that reduces the required radiant exposures of the individual phototherapies to achieve persistent vascular shutdown. PDL combined with anti-angiogenic agents is a promising strategy to achieve persistent vascular shutdown by preventing reformation and reperfusion of photocoagulated blood vessels. Integration of LSI into the clinical workflow may lead to surgical image guidance that maximizes acute photocoagulation, is expected to improve PWS therapeutic outcome. Continued integration of noninvasive optical imaging technologies and biochemical analysis collectively are expected to lead to more robust treatment strategies.

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Section: Development Of New Treatment Approachesmentioning

confidence: 99%

The Role of Laser Speckle Imaging in Port-Wine Stain Research: Recent Advances and Opportunities

Choi

Tan

Jia

et al. 2016

IEEE J. Select. Topics Quantum Electron.

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“…Combination of NPe6-PDT with surgical resection was found to achieve better therapeutic results than conventional protocols, particularly in patients with newly diagnosed malignant glioma [14]. We also examined the effect of NPe6-PDT on cell death modalities in glioma cells, with findings showing that NPe6-PDT induces mitochondrial apoptotic cell death accompanied by necrotic cell death [15].…”

Section: Introductionmentioning

confidence: 99%

Photodynamic therapy using talaporfin sodium induces concentration-dependent programmed necroptosis in human glioblastoma T98G cells

et al. 2015

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Photodynamic therapy (PDT) using photosensitizer induces several types of cell death, such as apoptosis, necrosis, and autophagy, depending on the PDT procedure, photosensitizer type, and cell type. We previously demonstrated that PDT using the photosensitizer talaporfin sodium (mono-L-aspartyl chlorine e6, NPe6; NPe6-PDT) induces both mitochondrial apoptotic and necrotic cell death in human glioblastoma T98G cells. However, details regarding the mechanism of necrosis caused by NPe6-PDT are unclear. Here, we investigated whether or not necroptosis, a recently suggested form of programmed necrosis, is involved in the necrotic cell death of NPe6-PDT-treated T98G cells. Leakage of lactate dehydrogenase (LDH) from the cell layer into conditioned medium was significantly increased by NPe6 (25 and 50 μg/ml)-PDT, indicating that NPe6-PDT induces necrosis in these cells. NPe6 (25 μg/ml)-PDT treatment also induced conversion of microtubule-associated protein 1 light-chain 3 (LC3)-I into phosphatidylethanolamine-conjugated LC3-II accompanying autophagosome formation, indicators of autophagy; however, of note, NPe6 (50 μg/ml)-PDT did not induce such autophagic changes. In addition, both necrostatin-1 (a necroptosis inhibitor) and knockdown of necroptotic pathway-related proteins [e.g., receptor interacting serine-threonine kinase (RIP)-1, RIP-3, and mixed lineage kinase domain-like protein (MLKL)] inhibited leakage of LDH caused by NPe6 (25 μg/ml)-PDT. Taken together, the present findings revealed that NPe6-PDT-induced necrotic cell death is mediated in part by the necroptosis pathway in glioblastoma T98G cells.

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“…PDT has the potential to become a useful cancer therapy, as tumor cell death can be selectively induced via PDT (Wilson, 1992). We recently examined the safety and efficacy of PDT using talaporfin sodium (mono-L-aspartyl chlorine e6, NPe6) as an additional intraoperative treatment for malignant glioma patients and found that PDT, in addition to surgical resection, achieved better therapeutic results than conventional protocols, particularly in patients with newly diagnosed malignant glioma (Akimoto et al, 2012). We are therefore now investigating useful methods of talaporfin sodium-mediated PDT (NPe6-PDT) to provide more effective and safe treatment for glioma patients.…”

Section: Introductionmentioning

confidence: 99%

“…These observations indicate that necrotic cell death induces delayed obstacles after radiation therapy for the brain. In addition, necrosis may injure normal brain tissue via induction of occlusion or shut down of brain vasculature (Akimoto et al, 2012). Thus, positive induction of apoptotic, and not necrotic, cell death may improve the quality of life of glioma patients who undergo NPe6-PDT.…”

Section: Introductionmentioning

confidence: 99%

Effect of talaporfin sodium-mediated photodynamic therapy on cell death modalities in human glioblastoma T98G cells

et al. 2014

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-While photodynamic therapy (PDT) is an effective treatment for glioma, induction of apoptotic cell death of glioma cells is important for ensuring efficacy and safety of PDT treatment in glioma patients, as necrotic cell death can induce late appearance of obstacles in treatment. Here, we investigated the relationship between type of cell death and PDT treatment conditions involved in laser and photosensitizer dosage in human glioblastoma T98G cells. Photosensitizer talaporfin sodium-mediated PDT (NPe6-PDT) treatment induced laser and NPe6 dose-dependent cell death in T98G cells, whereas almost all cells pretreated with NPe6 at ≥ 30 μg/mL were killed by laser irradiation, regardless of laser dose. Morphological analysis showed that combination of high doses of NPe6 and laser irradiation changes the dominant cell death process from apoptosis to necrosis. Biochemical analysis (detection of caspase-3 activity and staining of cell surface-exposed phosphatidylserine) also showed that increasing laser dose changes the type of cell death from apoptotic to necrotic cell death after high-dose treatment with NPe6. Lactate dehydrogenase leakage assay demonstrated that a laser dose of 5 J/cm 2 induced less leakage than 30 J/cm 2 . Our results suggested that type of glioma cell death in NPe6-PDT changed with fluctuations in laser and NPe6 dose, and that combination of 30 μg/mL NPe6 with 5 J/cm 2 laser is the best treatment condition for inducing an increase in apoptotic cells while keeping rate of necrotic cell death low in this in vitro study.

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Preliminary clinical report on safety and efficacy of photodynamic therapy using talaporfin sodium for malignant gliomas

Cited by 114 publications

References 17 publications

The Role of Laser Speckle Imaging in Port-Wine Stain Research: Recent Advances and Opportunities

The Role of Laser Speckle Imaging in Port-Wine Stain Research: Recent Advances and Opportunities

Photodynamic therapy using talaporfin sodium induces concentration-dependent programmed necroptosis in human glioblastoma T98G cells

Effect of talaporfin sodium-mediated photodynamic therapy on cell death modalities in human glioblastoma T98G cells

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