Preliminary experiment for cell count using flow cytometry

Morono, Yuki; Kallmeyer, Jens; Inagaki, Fumio

doi:10.2204/iodp.proc.329.110.2011

Cited by 7 publications

(4 citation statements)

References 8 publications

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“…Total prokaryotic cells were enumerated in triplicate from 13 uncontaminated sediment samples collected all along the core, using the cell extraction protocol (protocol FCM-A) described by (Morono et al, 2011) until step 9. Then, all supernatants containing extracted cells were filtered onto 0.2-mm filters (Anodisc, Whatman, Versailles, France) and stained with SYBRGreen I (Invitrogen, Cergy Pontoise, France), as described elsewhere (Noble and Fuhrman, 1998).…”

Section: Cell Countsmentioning

confidence: 99%

“…We analyzed and compared cell abundances and cell concentrations reported for different geographic sites using a standardized procedure based on cell extraction and dissolution of silicates (Noble and Fuhrman, 1998;Kallmeyer et al, 2008;Morono et al, 2011) (Figure 2). Mean cell numbers decreased with depth from about 1.5 Â 10 6 ± 4.7 Â 10 4 cells cm À 3 (n ¼ 8) at the surface (3.76 and 15.1 m CSF-A) to 2.5 Â 10 4 ±4.9 Â 10 3 cells cm À 3 (n ¼ 7) within the deepest samples (1911 and 1922 m CSF-A).…”

Section: Vertical Distribution Of Cellsmentioning

confidence: 99%

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Microorganisms persist at record depths in the subseafloor of the Canterbury Basin

et al. 2014

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The subsurface realm is colonized by microbial communities to depths of 41000 meters below the seafloor (m.b.sf.), but little is known about overall diversity and microbial distribution patterns at the most profound depths. Here we show that not only Bacteria and Archaea but also Eukarya occur at record depths in the subseafloor of the Canterbury Basin. Shifts in microbial community composition along a core of nearly 2 km reflect vertical taxa zonation influenced by sediment depth. Representatives of some microbial taxa were also cultivated using methods mimicking in situ conditions. These results suggest that diverse microorganisms persist down to 1922 m.b.sf. in the seafloor of the Canterbury Basin and extend the previously known depth limits of microbial evidence (i) from 159 to 1740 m.b.sf. for Eukarya and (ii) from 518 to 1922 m.b.sf. for Bacteria.

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Section: Cell Countsmentioning

confidence: 99%

Section: Vertical Distribution Of Cellsmentioning

confidence: 99%

Microorganisms persist at record depths in the subseafloor of the Canterbury Basin

et al. 2014

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“…Details of their application are provided in the "Methods" chapter of the IODP Proceedings (Exp. 329 Scientists, 2011), particularly in the sections on biogeochemistry, microbiology, and physical properties.…”

Section: Technical Advancesmentioning

confidence: 99%

IODP Expedition 329: Life and Habitability Beneath the Seafloor of the South Pacific Gyre

2013

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Integrated Ocean Drilling Program (IODP) Expedition 329 made major strides toward fulfilling its objectives. Shipboard studies documented (1) fundamental aspects of habitability and life in this very low activity subseafloor sedimentary ecosystem and (2) first-order patterns of habitability within the igneous basement. A broad range of postexpedition studies will complete the expedition objectives. Throughout the South Pacific Gyre (SPG; Sites U1365–U1370), dissolved oxygen and nitrate are present throughout the entire sediment sequence, and sedimentary microbial cell counts are lower than at all previously drilled IODP/ Ocean Drilling Program (ODP)/Deep Sea Drilling Program (DSDP) sites. In contrast, at Site U1371 in the upwelling zone just south of the gyre, detectable oxygen and nitrate are limited to the top and bottom of the sediment column, manganese reduction is a prominent electron-accepting process, and cell concentrations are higher than at the same depths in the SPG sites throughout the sediment column. Geographic variation in subseafloor profiles of dissolved and solid-phase chemicals are consistent with the magnitude of organic-fueled subseafloor respiration declining from outside the gyre to the gyre center. <br><br> Chemical profiles in the sedimentary pore water and secondary mineral distributions in the basaltic basement indicate that basement alteration continues on the timescale of formation fluid replacement, even at the sites with the oldest basement (84–120 Ma at Sites U1365 and U1366). <br><br> doi:<a href="http://dx.doi.org/10.2204/iodp.sd.15.01.2013" target="_blank">10.2204/iodp.sd.15.01.2013</a>

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“…Time scales consecrated to cultivation might be inadequate to scientist's careers productivity standards. Nevertheless, a fraction of microbial cells maintain potential for metabolic activity [22,25] and at least a fraction of subsurface microbes can be adapted to laboratory growth conditions [26][27][28]. Subseafloor sediments harbor as much as endospores as vegetative cells [22].…”

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confidence: 99%