Preliminary findings on the expression of thymosin beta-10 in human breast cancer

Verghese-Nikolakaki, S.; Apostolikas, N.; Livaniou, Evangelia; Ithakissios, Dionyssis S.; Evangelatos, Gregory P.

doi:10.1038/bjc.1996.562

Cited by 41 publications

(32 citation statements)

References 12 publications

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“…Thymosin b-10 has also been proposed to have dual functions: programmed cell death (Hall, 1995) and invasion or metastasis (Verghese-Nikolakaki et al, 1996;Marian et al, 1993). Whereas thymosin b-10 may contribute to invasion or metastasis by some cancer types, our results demonstrated its involvement in apoptosis in ovarian tissue.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of the thymosin β-10 gene in human ovarian cancer cells disrupts F-actin stress fiber and leads to apoptosis

et al. 2001

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Section: Discussionmentioning

confidence: 99%

Overexpression of the thymosin β-10 gene in human ovarian cancer cells disrupts F-actin stress fiber and leads to apoptosis

et al. 2001

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“…The peak may represent thymosin h-10, a member of a family of highly conserved small acid peptides that control the growth and differentiation of many cell types (40,41). Thymosin h-10 can act as a major actin-sequestering factor (42) and is suggested to play a role in invasion and metastasis (40,41). Thymosin h-10 is highly expressed in fetal tissues and down-regulated in normal adult tissues (43); however, its overexpression is seen in many tumors, including breast cancer.…”

Section: Discussionmentioning

confidence: 99%

Proteomic Profiling of Primary Breast Cancer Predicts Axillary Lymph Node Metastasis

et al. 2006

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To determine if protein expression in primary breast cancers can predict axillary lymph node (ALN) metastasis, we assessed differences in protein expression between primary breast cancers with and without ALN metastasis using surfaceenhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS). Laser capture microdissection was performed on invasive breast cancer frozen sections from 65 patients undergoing resection with sentinel lymph node (SLN) or level I and II ALN dissection. Isolated proteins from these tumors were applied to immobilized metal affinity capture (IMAC-3) ProteinChip arrays and analyzed by SELDI-TOF-MS to generate unique protein profiles. Correlations between unique protein peaks and histologically confirmed ALN status and other known clinicopathologic factors were examined using ANOVA and multivariate logistic regression. Two metalbinding polypeptides at 4,871 and 8,596 Da were identified as significant risk factors for nodal metastasis (P = 0.034 and 0.015, respectively) in a multivariate analysis. Lymphovascular invasion (LVI) was the only clinicopathologic factor predictive of ALN metastasis (P = 0.0038). In a logistic regression model combining the 4,871 and 8,596 Da peaks with LVI, the area under the receiver operating characteristic curve was 0.87. Compared with patients with negative ALN, those with z2 positive ALN or non-SLN metastases were significantly more likely to have an increased peak at 4,871 Da (P = 0.016 and 0.0083, respectively). ProteinChip array analysis identified differential protein peaks in primary breast cancers that predict the presence and number of ALN metastases and non-SLN status. (Cancer Res 2006; 66(24): 11825-30)

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“…Additional members of the β-thymosin family have been identified and these peptides exhibit similar properties to thymosin β 4 . Thymosin β 4 and other β-thymosins appear to be involved in a number of different processes like cancerogenesis (Hall, 1991;Bao et al, 1996;Verghese-Nikolakaki et al, 1996;Califano et al, 1998;Kobayashi et al, 2002;Otto et al, 2002) and apoptosis (Iguchi et al, 1999;Niu and Nachmias, 2000;Müller et al, 2003). In the extracellular space, thymosin β 4 participates in several physiological processes, e.g.…”

Section: Introductionmentioning

confidence: 99%

Nuclear localisation of the G-actin sequestering peptide thymosin β4

Huff

Rosorius

Otto

et al. 2004

Journal of Cell Science

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Thymosin β4 is regarded as the main G-actin sequestering peptide in the cytoplasm of mammalian cells. It is also thought to be involved in cellular events like cancerogenesis, apoptosis, angiogenesis, blood coagulation and wound healing. Thymosin β4 has been previously reported to localise intracellularly to the cytoplasm as detected by immunofluorescence. It can be selectively labelled at two of its glutamine-residues with fluorescent Oregon Green cadaverine using transglutaminase; however, this labelling does not interfere with its interaction with G-actin. Here we show that after microinjection into intact cells, fluorescently labelled thymosin β4 has a diffuse cytoplasmic and a pronounced nuclear staining. Enzymatic cleavage of fluorescently labelled thymosin β4 with AsnC-endoproteinase yielded two mono-labelled fragments of the peptide. After microinjection of these fragments, only the larger N-terminal fragment, containing the proposed actin-binding sequence exhibited nuclear localisation, whereas the smaller C-terminal fragment remained confined to the cytoplasm. We further showed that in digitonin permeabilised and extracted cells, fluorescent thymosin β4 was solely localised within the cytoplasm, whereas it was found concentrated within the cell nuclei after an additional Triton X100 extraction. Therefore, we conclude that thymosin β4 is specifically translocated into the cell nucleus by an active transport mechanism, requiring an unidentified soluble cytoplasmic factor. Our data furthermore suggest that this peptide may also serve as a G-actin sequestering peptide in the nucleus, although additional nuclear functions cannot be excluded.

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Preliminary findings on the expression of thymosin beta-10 in human breast cancer

Cited by 41 publications

References 12 publications

Overexpression of the thymosin β-10 gene in human ovarian cancer cells disrupts F-actin stress fiber and leads to apoptosis

Overexpression of the thymosin β-10 gene in human ovarian cancer cells disrupts F-actin stress fiber and leads to apoptosis

Proteomic Profiling of Primary Breast Cancer Predicts Axillary Lymph Node Metastasis

Nuclear localisation of the G-actin sequestering peptide thymosin β4

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