Preliminary phytochemical and antibacterial screening of crude extract of the leaf ofAdhatoda vasica. L

Abstract

The fresh leaves were dried for 6 hours at 50-60°C. The dried samples were then crushed into powder using an electronic blender. The powdered sample was stored in a bottle at room temperature, prior to analysis. Preparation of Extracts Ethanol extractionA powdered sample of 100 gm was weighed and soaked in 250 ml of 95% ethanol in a separating funnel for 24 hours, with intermittent shaking. The plant extract was then collected and filtered through Whatman No.1 filter paper. The extract was concentrated at 50°C using a rotatory evaporator and then air-dried. The dried powder was stored at 40°C in an airtight bottle. Similarly, the procedure was repeated with petroleum ether and water as solvents, using 100 gm of the fresh ground sample, for each extraction. All the extracts were cooled at room temperature. [2] Phytochemical Analysis The extracts were analyzed for the presence of phenols, tannins, alkaloids, anthraquinones, saponins, flavanoids, aminoacids and reducing sugars, using the standard procedure. [3] Agar-well Diffusion MethodThe antimicrobial activity was carried out by the agar-well diffusion assay using Muller Hinton agar plates. [4] The plates were swabbed with S. aureaus,

Antimicrobial Effect of Aqueous, Ethanol, Methanol and Glycerin Extracts of Satureja bachtiarica on Streptococcus pyogenes, Pseudomonas aeruginosa and Staphylococcus epidermidis

“…The curative properties of medicinal plants are due to the presence of various complex chemical substances of different composition which occur as secondary metabolites [22,23]. In conclusion, it can suggest that S. bachtiarica extract "in vitro" have considerable antimicrobial ability over the studied strains.…”

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“…It has great importance in Ayurveda due to its bronchodilatory effect. It also has anti-inflammatory, anti-bacterial activity 14 , anticestodal activity 15 , antileishmanial activity 16 and anti-helminthic activity 17 .…”

Evaluation of the Efficacy of a Polyherbal Formulation (Bovituss) as a Supportive Treatment in Respiratory Infections of Bovines

“…Bovituss is a scientific blend of various herbs and the clinical action produced by it is due to the varied action of the constituent herbs which act synergistically for better and faster recovery in respiratory tract infection. The key herbs present are Adhatoda vasica having antitussive ( Jahan and Siddiqui, 2012) and anti-bacterial (Karthikeyan et al, 2014) properties; Ocimun sanctum having immunomodulatory (Mukherjee et al, 2005) and anti-inflammatory (Kothari and Sharma, 2012) properties; Hedychium spicatum showing bronchodilator, anti-inflammatory and analgesic activity (Ghildiyal et al, 2012); and Glycyrrhiza glabra showing anti-tussive ( Jahan and Siddique, 2012), anti-inflammatory (Mirmala and Selvaraj, 2011), and anti-bacterial (Nitalikar et al, 2011) activity. …”