Preliminary Phytochemical Screening and in Vitro Antimicrobial Activity of Ethanolic Extract of Stem of the Herb Basella Alba L. Var Rubra (L.) Stewart (Family-Basellaceae)

Abstract

Objective: To evaluate the antimicrobial activity ethanolic extracts of Basella alba L. var. rubra (L.) stewart (family-basellaceae) along with phytochemical analysis. Methods:The ethanolic extract of stems of the herb Basella alba L. var. rubra (L.) stewart (family-basellaceae) were prepared and analysed for phytochemical constituents using standard methods. The antimicrobial activity of the plant extracts were examined against 2 bacterial strains among one is gram positive and other is gram negative and2 fungal strains using agar well diffusion method. Results:The present study describes the phytochemical profile and antimicrobial activity of Basella alba L. var. rubra (L.) stewart. Preliminary phytochemical screening of the extract were carried out and it revealed the presence of carbohydrates, tannins, terpene, saponin. The presence of these bioactive constituents is associated with the antimicrobial activity of the plant. The extract solvated by ethanol showed varying levels of antimicrobial activity against the tested bacteria namely E. coli, S. aureus, and fungi namely Candida albicans and Trichophyton rubrum. The study showed that the extract has a marked Sensitivity towards antibacterial strains namely E. coli, S. aureus, and antifungal strain namely Candida albicans and Trichophyton rubrum. Conclusion:The present study concluded ethanolic extract of stem of the Basella alba L. var. rubra (L.) Stewart revels many phytochemicals such as carbohydrate, tenin, saponin etc. This extract was found to possess promising antimicrobial activity when compared with the standards.

Structural Studies on Diverse Betacyanin Classes in Matured Pigment-Rich Fruits of Basella alba L. and Basella alba L. var. ‘Rubra’ (Malabar Spinach)

“…Recent reports on B. alba betacyanins [ 2 , 3 , 4 , 5 , 6 , 17 , 23 , 24 , 25 ] focused on the main pigment, gomphrenin, only scarcely mentioning some other acylated gomphrenins which were structurally identified two decades ago as the principal acylated pigments in purple Gomphrena globosa L. [ 7 , 29 ]. In this study, our detailed studies evidently detected new betacyanin pigments ( Table 1 , Figure 2 ) and also revealed completely novel structures with unique acylation patterns ( Table 2 and Table 3 ) as well as compared quantitatively the two plant varieties ( Section 2.7 ) [ 30 ].…”

“…Traditional medicine, especially in India and China, uses different parts of said plants to treat many diseases [ 2 ]. In addition to the content of vitamins and minerals, the extracts of these plants can be attributed to antimicrobial, [ 3 ] anti-inflammatory and antidepressant properties [ 2 ]. Valuable sources of pro-health substances are both the stems and leaves of Malabar spinach [ 4 ], but also extracts from its fruits, which have been proven, among others, to show cytotoxic properties against human cervical cancer cells [ 5 ].…”

Basella alba L. (Malabar Spinach) as an Abundant Source of Betacyanins: Identification, Stability, and Bioactivity Studies on Natural and Processed Fruit Pigments

“…The mobile phases were A, 2% formic acid in water and B, methanol. The gradient profile was (t (min), % B), (0, 10), (12,40), (15,80), (19,80). The full-range PDA signal was recorded, and individual chromatograms were displayed at 538, 505, 490, and 440 nm.…”

“…17 These plants are primarily cultivated in subtropical regions, where their potent health benefits have been acknowledged and well-regarded. 18 Their anti-inflammatory and antibacterial properties are highlighted, 19 but studies have also reported the cytotoxic effect of Malabar spinach fruit extracts on human cervical carcinoma cells. 20 Besides containing compounds such as carbohydrates, proteins, lipids, niacin, ascorbic acid, and tocopherols, Malabar spinach is also a rich source of betalain pigments.…”

An updated phytochemical and pharmacological review on Malabar Spinach (Basella alba or Basella rubra)

“…Whereas the standard drug gentamycin showed 29 and 23mm Zone of inhibition respectively at 20mg/ml. The maximum antifungal activity was also observed at 100mg/ml concentration i.e., 20.2 and 15.25mm zone of inhibition and standard drug fluconazole at concentration of 20mg/ml showed 24 and 22mm zone of inhibition against Candida albicans and Trichophyton rubrum respectively [20]. evaluated antimicrobial activity of B. alba fruit using agar well diffusion method against eight species of microorganisms.…”