Preliminary phytochemical screening, plant growth inhibition and antimicrobial activity studies of Faidherbia albida legume extracts

Abstract

Phytochemical analysis of the methanolic and aqueous extracts of Faidherbia albida legumes indicated the presence of terpenes, cardiac glycosides, monosaccharides and carbohydrates type of compounds in both extracts. While alkaloids and saponins were found in aqueous extract only, flavonoids were found to be absent in both extracts. The aqueous and methanolic extracts exhibited a potent growth stimulation effect. Inhibition of both the rootlet and shoot showed a dose dependent response. Aqueous extract has a greater inhibitory effect on rootlet growth than shoot growth. The methanolic extract has a greater inhibitory effect than the aqueous extract. Both extracts and some fractions were tested against three pathogenic bacterial species; Staphylococcus aureus, Escherichia coli and Shigella dysenteriae, also tested against three pathogenic fungal species; Fusarium oxysporum, Alternaria alternate, and Aspergillus niger. Most of the plant extracts stimulate the studied fungal growth specially the aqueous extract. Meanwhile it shows interesting results by inhibiting the growth of the studied pathogenic bacterial species with most extracts and fractions

Quantitative Phytochemical Estimation and Evaluation of Antioxidant and Antibacterial Activity of Methanol and Ethanol Extracts of Heliconia rostrata

“…The extracts were screened for the presence of alkaloids, glycosides, steroids and triterpenoids, flavonoids, proteins and amino acids, carbohydrates and reducing sugars, tannins and saponins using standard methods [15][16][17] .…”

“…Change of violet colour to blue indicated the presence of steroids [17] . To 5 mg of extract, 2 ml of chloroform was added followed by the addition of 1 ml of acetic anhydride and 1 ml of concentrated sulphuric acid.…”

“…To this mixture concentrated sulphuric acid was added through the walls of the test tube. Appearance of a violet ring indicates the presence of carbohydrates [17] . Fehling test was done by adding to 1 ml of Fehling A solution, 1 ml of Fehling B solution and boiling the mixture for a minute.…”

“…Then equal amount of extract solution was added and heated in a water bath for 5 to 10 min. Appearance of a brick red precipitate indicated the presence of reducing sugars in the extract [17] .…”

Anticancer Activity of Vernonia amygdalina Del. Extract on WiDr Colon Cancer Cell Line

“…Phytochemical analysis of n-hexane, ethyl acetate, and ethanol extracts was performed to obtain secondary metabolite information dissolved in each extract with different polarities. The secondary metabolites tested were alcaloids, flavonoids, saponins, glycosides, tannins, terpenes, and steroids [11].…”