Preliminary results on associations between leptin gene (<i>LEP</i>) and some reproduction performance traits of boars

Abstract

SummaryStudies on porcine genome contributed to identification of polymorphic loci of the genes that may influence the level of reproduction-related traits. The LEP gene codes for leptin -a protein secreted to blood from adipocytes, important for food intake, energy expenditure, and immune response; the protein also influences some functions of mammalian reproductive system. The aim of this study was to determine the polymorphism of HinfI in the leptin gene and its effect on selected qualitative and quantitative traits of the semen. The analyses included 54 AI boars, which had been derived from crossing Duroc with Pietrain breeds. The genotypes were established with PCR-RFLP. Two alleles LEP/HinfI were identified: LEP T (152 bp) and LEP C (68 and 84 bp) with the frequencies 0.713 and 0.287 respectively. The genotype frequencies were as follows:

Estrogen receptor gene (<i>ESR</i>) and semen characteristics of boars

“…In younger time studies on porcine genome contributed to identification of polymorphic loci of the genes that may influence the level reproduction-relates traits in sows (KMIEĆ, et al, 2001;LINVILLE, et al, 2001;DRÖGEMÜLLER et al, 1999) and boars (MAĆKOWSKI, et al, 2004;KMIEĆ et al, 2003;SCHLINGMANN et al, 2002;KMIEĆ et al, 2001). Progress in genetics and molecular techniques has enabled application of DNA polymorphism by selection of animals known as marker-assisted selection (MAS).…”

R159Q Polymorphism in Leptin Gene and Its Correlation with Semen Quality Parameters in Nili-Ravi Buffalo Bulls

“…Similarly, Hasanain et al (2017) and Dalvi et al (2018) reported non-significant (P<0.05) association of sperm concentration with prolactin and FSH beta gene, respectively. However, Kmieć et al (2003) reported a significant (P<0.05) predominance of LEP CT and TT genotypic boars in respect to sperm concentration in their ejaculates.…”

“…In this study, results of the semen quality parameters in buffalo bulls revealed non-significant (P>0.05) correlation between leptin gene polymorphism and ejaculatory volume. Kmieć et al (2003) investigated the effect of HinfI polymorphism of leptin gene and reported a significant (P<0.05) predominance of LEP CT and TT genotypic boars in respect to ejaculatory volume. However, association of FSH beta gene and prolactin gene polymorphism with semen volume was non-significant (Ghasemi and Ghorbani, 2014;Hasanain et al, 2017).…”

“…These results indicated that LEP gene markers could be used in selection programs in swine (Suwanasopee and Koonawootrittriron, 2011). Kmieć et al (2003) monitored the effects of HinfI polymorphism of LEP gene on some semen quality traits in AI boars and reported a significant (P≤0.05) predominance of LEP CT and TT genotypic boars with respect to ejaculatory volume, live sperm percentage and sperm concentration. Kanchan et al (2014) examined invitro capacitated and fresh ejaculated spermatozoa of buffalo bulls for the existence of leptin gene transcript.…”

Associations between polymorphism of the steroid 21-hydroxylase gene (&lt;i&gt;CYP21&lt;/i&gt;) and litter size of Polish Large White × Polish Landrace sows* (short communication)

“…Schlüsselwörter: Steroid-21-Hydroxylase-Gene, Schwein, Wurfgröße, Reproduktion, Polymorphismus Introduction Last years the identification of genes or markers associated with reproductive traits in swine is an important area of research (DROEGEMUELLER et al, 1999;KMIEĆ et al, 2001;SCHLINGMANN et al, 2002;KMIEĆ et al, 2003;MAĆKOWSKI et al, 2004;SCHWARZ et al, 2005) because the large economic impact these discoveries could have on the swine industry. One of the most important enzymatic complexes that take part in the synthesis of adrenal steroids is 21-hydroxylase (CYP21).…”