Preliminary Results on the Staining of Spores and other structures ofHomobasidiomycetes in Cotton Blue and its importance for Taxonomy

Kotlaba, František; Pouzar, Zdeněk

doi:10.1002/fedr.4880690204

Cited by 25 publications

(14 citation statements)

References 5 publications

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“…metachromatic reaction, is marked CRB+; blue reaction (orthochromatic) CRB-. For further references on the use of CB in Aphyllophorales, the reader is referred to Kotlaba and Pouzar (1964), Jülich (1974), and Niemelä (2005).…”

Section: Morphologymentioning

confidence: 99%

Obba and Sebipora, new polypore genera related to Cinereomyces and Gelatoporia (Polyporales, Basidiomycota)

Miettinen

Rajchenberg

2011

Mycol Progress

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Cinereomyces clade is a newly proposed monophyletic group of polypores containing currently four genera and five species, including two promising biopulping fungi, Ceriporiopsis rivulosa and C. subvermispora. The Cinereomyces clade is well-delimited in nrDNA-based phylogenetic analysis, but its position in Polyporales remains unclear. Its closest relative may be found in the core polyporoid clade. Only a few morphological characters are common for all the species in the clade, e.g. CB-and CRB+ hyphae, white fruiting bodies, presence of oil, and middlesized spores. Culturally, the species are unified by producing simple-septate generative hyhpae in the margin, which produce simple-clamped hyphae backwards. The genus Gelatoporia is the correct place for Ceriporiopsis subvermispora. Two new genera are described in the group: Obba to incorporate C. rivulosa and a new austral species, Obba valdiviana, known from southern Argentina and recorded here also from Tasmania, and Sebipora to accommodate a new species from tropical Asia, S. aquosa. ITS sequences imply that Eurasian Gelatoporia subvermispora may belong to a different species from the North American one. G. subvermispora is recorded as new to Indonesia.

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Section: Morphologymentioning

confidence: 99%

Obba and Sebipora, new polypore genera related to Cinereomyces and Gelatoporia (Polyporales, Basidiomycota)

Miettinen

Rajchenberg

2011

Mycol Progress

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“…Foram preparados cortes do material à mão livre, utilizando-se lâmina inoxidável, sob estereomicroscópio. Foram utilizadas solução de KOH 3%, reagente de Melzer e Azul de Algodão para verificar as reações de mudança de cor, amiloidia e cianofilia de micro estruturas como basidiósporos e hifas (MARTIN, 1934;KOTABLA;POUZAR, 1964;SINGER, 1986). A observação das microestruturas foi realizada em microscópio binocular Zeiss West Germany.…”

Section: Análise Morfológica Dos Espécimesunclassified

Seleção de Basidiomycetes da Amazônia para produção de enzimas de interesse biotecnológico

Souza¹,

Oliveira²,

Andrade³

2008

Ciênc. Tecnol. Aliment.

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A classe Basidiomycetes propriamente dita inclui os fungos em sua maioria saprófitos, que possuem basídios, corpos frutíferos que vão de alguns centímetros até quase um metro, todos visíveis a olho nu. A sistemática é feita pela morfologia do píleo, lamelas e poros .Os fungos têm sido bastante usados como produtores de diferentes substâncias de interesse econômico, tais como: enzimas, antibióticos, vitaminas, aminoácidos e esteróides (BRAGA; DESTÉFANO; MESSIAS, 1999). As enzimas são usadas, em grande escala, na indústria de tecidos (celulases), detergentes (proteases e lipases), de alimentos (amilases, pectinases, proteases e celulases) e de couro (proteases e lipases) (NIELSEN; OXENBOLL, 1998).Dentre as enzimas de importância industrial, produzidas por fungos, e de interesse na área de alimentos, foram abordadas neste trabalho as celulases, fenoloxidases, pectinases, amilases e proteases. AbstractMushrooms, edible basidiomycetes, have been extensively used as producers of different substances of economical interest, such as enzymes, antibiotics, vitamins, amino acids, and steroids. The objective of this study is to detect the production of enzymes such as amylases, proteases, cellulases, phenoloxidases and pectinases for lineages of basidiomycetes originating from the Amazonian forest. For the production of enzymes, mushrooms were cultivated in a liquid medium with substratum (0,5%), pH adjusted for each enzyme, and incubated at 28 °C, under agitation and 140 rpm for 96 or 120 hours. After this period, the samples were filtered for the separation of the mycelial mass. The filtrates were inoculated in solid medium surface perforated cup-plates of 6 mm diameter, appropriate for the detection of enzymes in Petri dishes. The plates were incubated at 28 °C for 24 hours, for observation to reveal enzymatic halos. It was verified the activity of the amylase and protease produced by the mushrooms grown in the liquid medium, with different nutritive sources. It was possible to detect the cellulase and protease production for all the ones that were isolated, 40% produced amylases, 50% produced phenoloxidases, and 10% produced pectinases. Regarding the activity of the amylase, the wheat bran substrate presented the largest degradation halos revealing Daedalea sp.4E6 and Daedalea sp. 1A, Stereaceae 22B and Pycnoporus sanguineus 12B mushrooms. Considering the substrata tested for protease production, it was observed that the fish protein concentrate was the best. The P. sanguineus 12B, Stereaceae 22B and Cantharellus guyanensis 4Bl mushrooms were the best protease producers. Keywords: enzymatic activity; mushrooms; biotechnology. ResumoOs fungos têm sido bastante usados como produtores de diferentes substâncias de interesse econômico, tais como: enzimas, antibióticos, vitaminas, aminoácidos e esteróides. Este estudo teve como objetivo detectar a produção de enzimas por linhagens de Basidiomycetes, oriundas de áreas de floresta da Amazônia. Para a produção de enzimas, os fungos foram cultivados em meio líquido adicionado de ...

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“…Line drawings were made with a camera lucida attachment. Cyanophily of basidiospore and hyphal walls was observed in 0.1% cotton blue in 60% lactic acid (Kotlaba & Pouzar 1964;Singer 1986). Q values were obtained from dividing average basidiospore length by width for at least 30 spores (Kirk et al 2008).…”

Section: Methodsmentioning

confidence: 96%

The genusDendrothele(Agaricales, Basidiomycota) in New Zealand

Nakasone

Burdsall

2011

New Zealand Journal of Botany

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Preliminary Results on the Staining of Spores and other structures ofHomobasidiomycetes in Cotton Blue and its importance for Taxonomy

Cited by 25 publications

References 5 publications

Obba and Sebipora, new polypore genera related to Cinereomyces and Gelatoporia (Polyporales, Basidiomycota)

Obba and Sebipora, new polypore genera related to Cinereomyces and Gelatoporia (Polyporales, Basidiomycota)

Seleção de Basidiomycetes da Amazônia para produção de enzimas de interesse biotecnológico

The genusDendrothele(Agaricales, Basidiomycota) in New Zealand

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