Preliminary Studies on the Antibacterial Mechanism of a New Plant-Derived Compound, 7-Methoxycoumarin, Against Ralstonia solanacearum

Han, Songting; Yang, Liang; Wang, Yao; Ran, Yuao; Li, Shili; Ding, Wei

doi:10.3389/fmicb.2021.697911

Cited by 20 publications

(19 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…nicotianae has a high survival ability in an environment with complex pathogenic factors, the use of traditional chemical control methods with streptomycin and thiodiazole‐copper for controlling bacterial wilt shows few positive effects in the field 48 . Based on this, botanical pesticides are gradually developed and applied, hydroxycoumarins, 7‐methoxycoumarin, caffeic acid could be applied as potential protective agents in the management of tobacco bacterial wilt 46,49,50 …”

Section: Discussionmentioning

confidence: 99%

Antibacterial activity and action mechanism of curcusionol from Carex siderosticta Hance against Ralstonia nicotianae

Ren,

Zhou,

Liu

et al. 2023

Pest Management Science

View full text Add to dashboard Cite

BACKGROUNDTobacco bacterial wilt is a typical soil‐borne disease caused by Ralstonia nicotianae, which causes huge losses in tobacco production every year. The crude extract of Carex siderosticta Hance was shown to have antibacterial activity against R. nicotianae during our search, and the natural antibacterial components were sought after using bioassay‐guided fractionation of the compounds.RESULTEthanol extract of Carex siderosticta Hance with the minimum inhibitory concentration (MIC) value of 100 μg/mL against R. nicotianae in vitro. The potential of these compounds as antibactericides against R. nicotianae were assessed. Curcusionol (1), showed the highest antibacterial activity against R. nicotianae with MIC value of 12.5 μg/mL in vitro. In the protective effect tests, the control effect of curcusionol (1) was 92.31 and 72.60%, respectively, after application of 7 and 14 days, at a concentration of 1500 μg/mL, being comparable to that of streptomycin sulfate at a concentration of 500 μg/mL, confirming that curcusionol (1) showed the potential for the development of new antibacterial drugs. RNA‐sequencing, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) analysis confirmed that curcusionol mainly destroys R. nicotianae cell membrane structure and affects quorum sensing (QS) to inhibit pathogenic bacteria.CONCLUSIONThis study revealed that the antibacterial activity of Carex siderosticta Hance makes it a botanical bactericide against R. nicotianae, while curcusionol as lead structures for antibacterial development is obvious by its potent antibacterial activity. © 2023 Society of Chemical Industry.

show abstract

Section: Discussionmentioning

confidence: 99%

Antibacterial activity and action mechanism of curcusionol from Carex siderosticta Hance against Ralstonia nicotianae

Ren,

Zhou,

Liu

et al. 2023

Pest Management Science

View full text Add to dashboard Cite

show abstract

“…They were suitable for the treatment of in uenza, bronchial wheezing, allergic reaction and other bacteria(A et al 2021). 7-Methoxycoumarin, which could be used as an organic synthesis intermediate, was also a drug with the effect of at asthma, expectorant, cough suppression (Han et al 2021). The basic function of melatonin was to participate in the antioxidant system and prevent cells from causing oxidative damage ,which was the strongest endogenous free radical scavenger ever found.…”

Section: Discussionmentioning

confidence: 99%

Analysis of primary metabolites of Morchella fruit bodies and mycelium based on widely targeted metabolomics

Yang

Wang³

et al. 2021

Arch Microbiol

View full text Add to dashboard Cite

Morchella is a kind of medicinal and edible homologous fungia that is rich in multiple metabolites. The metabolites from Morchella are a kind of essential substance because of their biological activities. In this study, Morchella fruit bodies and mycelium were selected to identify their metabolites. The primary metabolites of the two experimental group were analyzed using a method of widely targeted metabolome based on UPLC-ESI-MS/MS. A total of 354 different metabolites, including 188 upregulated metabolites and 166 downregulated metabolites, were characterized. Further, the main 20 metabolic pathways of the metabolites were analyzed. The rst 9 ones are tyrosine metabolites, thyroid hormone biosynthetic pathway, phenylalanine metabolites, linoleic metabolites synthetic pathway, glycerophosphate metabolic pathway, choline in tumors, methyl butyl metabolites, arginine synthetic pathway, arginine, arginine and proline metabolites. This study provides theoretical basis for the analysis of metabolic pathway of Morchella fruit bodies and mycelium that serving for further research of their medicinal mechanism and effective components.

show abstract

“…6-Methylcoumarin causes cell elongation, disrupts cell division, and inhibits the expression of ftsZ, the gene encoding cytokinin (Yang et al, 2021b). 7-methoxycoumarin inhibits the growth of R. solanacearum (Yang et al, 2021a) and suppresses its virulence-related genes epsE, hrpG and popA (Han et al, 2021). Hydroxycoumarins can inhibit the expression of R. solanacearum flagellar genes fliA and flhC, and disrupt their cell membranes and inhibit biofilm formation (Yang et al, 2016).…”

Section: Plant Metabolites Involved In the Fight Against Ralstonia So...mentioning

confidence: 99%

“…7-methoxycoumarin inhibits the growth of R . solanacearum ( Yang et al, 2021a ) and suppresses its virulence-related genes epsE , hrpG and popA ( Han et al, 2021 ). Hydroxycoumarins can inhibit the expression of R .…”

Section: Plant Metabolites Involved In the Fight Against Ra...mentioning

confidence: 99%

Induced defense strategies of plants against Ralstonia solanacearum

et al. 2023

View full text Add to dashboard Cite

Plants respond to Ralstonia solanacearum infestation through two layers of immune system (PTI and ETI). This process involves the production of plant-induced resistance. Strategies for inducing resistance in plants include the formation of tyloses, gels, and callose and changes in the content of cell wall components such as cellulose, hemicellulose, pectin, lignin, and suberin in response to pathogen infestation. When R. solanacearum secrete cell wall degrading enzymes, plants also sense the status of cell wall fragments through the cell wall integrity (CWI) system, which activates deep-seated defense responses. In addition, plants also fight against R. solanacearum infestation by regulating the distribution of metabolic networks to increase the production of resistant metabolites and reduce the production of metabolites that are easily exploited by R. solanacearum. We review the strategies used by plants to induce resistance in response to R. solanacearum infestation. In particular, we highlight the importance of plant-induced physical and chemical defenses as well as cell wall defenses in the fight against R. solanacearum.

show abstract

Preliminary Studies on the Antibacterial Mechanism of a New Plant-Derived Compound, 7-Methoxycoumarin, Against Ralstonia solanacearum

Cited by 20 publications

References 47 publications

Antibacterial activity and action mechanism of curcusionol from Carex siderosticta Hance against Ralstonia nicotianae

Antibacterial activity and action mechanism of curcusionol from Carex siderosticta Hance against Ralstonia nicotianae

Analysis of primary metabolites of Morchella fruit bodies and mycelium based on widely targeted metabolomics

Induced defense strategies of plants against Ralstonia solanacearum

Contact Info

Product

Resources

About