Preliminary studies on therapeutic effect of ethanolic extract of Tylophora villosa leaves against paracetamol-induced hepatotoxicity in mice

Abstract

This study intended to investigate the therapeutic effect of ethanolic extract of Tylophora villosa leaves (E2TL) against paracetamol (PC)-induced hepatotoxicity (PCIH) in mice ( Mus musculus ). PCIH were generated using daily 250 mg/kg body weight (bw) PC administration by gavage for seven days, and then daily 27.5; 55.0; 82.5; 110.0; or 220.0 mg/kg bw E2TL were treated by gavage for seven or fourteen days. Meanwhile, the controls were given solvent only in the same manner. Mortality, blood glucose, and condition (color, weight, volume) of the livers were observed on day 15 (D15). Serum glutamate pyruvate transaminase (SGPT) and serum glutamate oxaloacetate transaminase (SG0T) were examined on D15, D22, and D30, and then malondialdehyde (MDA) was determined on D15. Results of this study revealed that on D15, the dosage of 110.0 mg/kg bw E2TL most effectively decreased MDA due to PCIH, from 6.78 ± 1.70 μmol/L to 3.45 ± 0.43 μmol/L, approaching the control condition (2.45 ± 0.05 μmol/L). PC administration was really toxic dosage and caused 13.3 % mortality. Blood glucose, weight, and volume of the liver decreased as the effect of PC administration, and then 220.0 mg/kg bw E2TL treatment could recover the condition as well as the controls. Color of the liver indicated a similar recovery by E2TL treatment. SGPT and SG0T increased significantly by PC administration, and this PCIH facts could be recovered gradually near the controls according to the dosages (55.0; 110.0; or 220.0 mg/kg bw) and duration (seven or fourteen days) of E2TL treatment. It could be concluded that E2TL showed therapeutic effect against PCIH in M. musculus .

Leaf ethanolic extract of Etlingera hemesphaerica Blume mitigates defects in fetal anatomy and endochondral ossification due to mercuric chloride during the post-implantation period in Mus musculus

“…This study was carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health This study was conducted by following the ethics of animal use, including aspects of the humane treatment of animals, in accordance with the principle of 5F (Freedom), namely; (a) free from hunger and thirst, (b) free from discomfort, (c) free from pain, injury and disease, (d) free from fear and the long-term stress, (e) freely expressing behavior naturally, given space and appropriate facilities [ 27 – 29 ]. The protocol was approved by the Committee on the Ethics of Animal Experiments of Bengkulu University.…”

OPTIMASI EKSTRAKSI DAUN NANGKA KUNING (Vincetoxicum villosum (Blume) Kuntze) DARI BERBAGAI PELARUT DAN METODE

“…Kemudian, hasil skrining fitokimia untuk ekstrak dengan pelarut etanol menunjukkan positif pada uji alkaloid dan tani serta hasil negatif pada uji steroid, flavonoid dan saponin. Sementara itu, dari penelitian sebelumnya diketahui bahwa ekstrak etanol menunjukkan hasil positif pada uji alkaloid, flavonoid dan triterpenoid serta hasil negatif untuk saponin dan steroid (Ruyani et. al, 2019).…”

“…Kajian menarik lainnya adalah mengenai organ target pada tubuh yang menjadi tujuan penggunaan ekstrak daun nangka kuning oleh masyarakat lokal dan ekstrak dari penelitian sebelumnya. Masyarakat lokal biasanya menggunakan ekstrak daun nangka kuning untuk mengobati penyakit kuning yang disebabkan oleh kerusakan pada hepar (Ruyani et. al, 2019).…”