Preliminary study of the influence of red blood cells morphometry on the species determinism of domestic animals

Adili, Nezar; Marrakchi, M.

doi:10.14202/vetworld.2014.219-223

Cited by 8 publications

(11 citation statements)

References 20 publications

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“…This mean value is smaller than the reported average diameter of bovine red blood cells (5.05 μm), but within the reported SD (± 0.16 μm) [62]. The hydrodynamic peak ( d peak ) diameters, as determined by DLS technique, were ≈ 164 nm (polydispersity index (PDI) = 0.10) and 91nm (PDI = 0.07) for the populations of nano-sized respectively (figure 2(b)).…”

Section: Resultssupporting

confidence: 67%

Optical properties of biomimetic probes engineered from erythrocytes

et al. 2016

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Light-activated theranostic materials offer a potential platform for optical imaging and phototherapeutic applications. We have engineered constructs derived from erythrocytes, which can be doped with the FDA-approved near infrared (NIR) chromophore, indocyanine green (ICG). We refer to these constructs as NIR erythrocyte-mimicking transducers (NETs). Herein, we investigated the effects of changing the NETs mean diameter from micron- (≈ 4 μm) to nano- (≈ 90 nm) scale, and the ICG concentration utilized in the fabrication of NETs from 5 to 20 μM on the resulting absorption and scattering characteristics of the NETs. Our approach consisted of integrating sphere-based measurements of light transmittance and reflectance, and subsequent utilization of these measurements in an inverse adding-doubling algorithm to estimate the absorption (μa) and reduced scattering (μs′) coefficients of these NETs. For a given NETs diameter, values of μa increased over the approximate spectral band of 630 – 860 nm with increasing ICG concentration. Micron-sized NETs produced the highest peak value of μa when using ICG concentrations of 10 and 20 μM, and showed increased values of μs′ as compared to nano-sized NETs. Spectral profiles of μs′ for these NETs showed a trend consistent with Mie scattering behavior for spherical objects. For all NETs investigated, changing the ICG concentration minimally affected the scattering characteristics. A Monte Carlo-based model of light distribution showed that the presence of these NETs enhanced the fluence levels within simulated blood vessels. These results provide important data towards determining the appropriate light dosimetry parameters for an intended light-based biomedical application of NETs.

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Section: Resultssupporting

confidence: 67%

Optical properties of biomimetic probes engineered from erythrocytes

et al. 2016

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“…This work is the continuity of the preliminary study realized only in females, which allowed us to seem very highly significant differences in the morphometry of erythrocytes between species [ 13 ]. The current study was performed in males and females of several breeds of cattle, sheep, goats, horses, and dogs; it aims to complete and confirm the results already published and also to define and develop criteria selection of domestic animals species, by considering three morphometric parameters of red blood cells which are the diameter, the circumference, and the surface.…”

Section: Introductionmentioning

confidence: 83%

“…The study of the morphometric parameters of red blood cells was performed with special OPTIKA™ Vision Pro software, of the optical microscope OPTIKA B-350 [ 13 ]. Initially, the diameter of red blood cells was measured, which is still estimated in relation to the shape of the cell; then, two new parameters that have not previously treated were developed, which are the circumference and the surface of erythrocytes.…”

Section: Methodsmentioning

confidence: 99%

Species determination using the red blood cells morphometry in domestic animals

Adili¹,

Marrakchi²,

Belabbas³

2016

Vet World

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Aim:This investigation is placed in the context of continuity of a preliminary study already published; it was conducted in cattle, sheep, goats, horses, and dogs; the main aim is to reveal and develop criteria for the animal species determination based on the morphometric parameters of red blood cells.Materials and Methods:Blood samples were taken from the jugular vein; and the smears were confectioned on slides immediately after the blood collection and stained according to the May-Gründwald Giemsa method. For the morphometric study, three parameters were considered which are: The diameter, the circumference, and the surface of erythrocytes; and measurements were achieved using the OPTIKATM Vision Pro software. Statistical analysis was performed by both analysis of variances and Student’s t analytical tests.Results:The recorded data showed that the three morphometric parameters of red blood cells are higher in dogs followed, respectively, by those of horses, cattle, and sheep, whereas, the goats have the lowest ones. In addition, the obtained results allowed us to propose new reference values for the circumference and the surface of erythrocyte in considered species.Conclusion:This investigation permit concluding that from a drop of blood it is possible to characterize the different animal species, taking into account the diameter, the circumference, and the surface of erythrocytes.

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“…Because red blood cells in ruminants have a life span of about 130-150 days (Adili and Melizi, 2014), levels of these elements in whole blood often change with a slow rate. Consequently, the experimental diets enriched with SeY or SeVI revealed negligible influence on the concentrations of macro bioelements (Ca and Mg) and trace toxic metals (i.e.…”

Section: Discussionmentioning

confidence: 99%