Preliminary study on Emodin alleviating alpha-naphthylisothiocyanate-induced intrahepatic cholestasis by regulation of liver farnesoid X receptor pathway

Abstract

The aim of this study is to investigate Emodin on alleviating intrahepatic cholestasis by regulation of liver farnesoid X receptor (FXR) pathway. Cell and animal models of intrahepatic cholestatis were established. Biochemical tests and histomorphology were performed. The messenger RNA (mRNA) and protein expression of FXR, small heterodimer partner (SHP), uridine diphosphate glucuronosyltransferase 2 family polypeptide B4 (UGT2B4), and bile salt export pump (BSEP) was detected. As a result, compared with the model group, the serum levels of biochemical test were significantly lower in the Emodin group (P <0.01). The histopathological changes were remitted significantly by Emodin treatment. In the model group, the mRNA and protein expression of FXR, SHP, UGT2B4, and BSEP was significantly lower than in the normal group in cell models (P <0.05). With Emodin intervention, the expression of FXR, SHP, UGT2B4, and BSEP was notably increased (P <0.05). In conclusion, Emodin plays a protective role in intrahepatic cholestasis by promoting FXR signal pathways.

Li-Dan-He-Ji Improves Infantile Cholestasis Hepatopathy Through Inhibiting Calcium-Sensing Receptor-Mediated Hepatocyte Apoptosis

“…In vivo study, we used ANIT to establish the rat model of hepatotoxicity (Ding et al, 2016;Xiong et al, 2019) and evaluate the effects of LDHJ granules on intrahepatic cholestasis (Kossor et al, 1993). Consistent with the previously reported findings (Wang et al, 2014), the increased levels of biomarkers (TBA, TBIL, DBIL, AST, ALT, ALP, and g-GT) and abnormal histopathological changes were observed in the liver of young rats with ANIT-induced intrahepatic cholestasis.…”

“…The protective effects of forsythoside-A and chlorogenic acid on cell apoptosis have been reported (Ali et al, 2017;Yan et al, 2017). Our research group has found the rescue activity of emodin on the intrahepatic cholestasis (Ding et al, 2016;Xiong et al, 2019). So forsythoside-A, emodin, chlorogenic acid may be promising in representing the LDHJ for further cell experiments.…”

“…Primary hepatocytes were extracted from the livers of 4-week-old SD rats using a modified two-step collagenase perfusion technique (Reinehr et al, 2006). This study also was approved by the Institutional Animal Care and Use Committee at Tongji Medical College, Huazhong University of Science and Technology (2016IACUC Number: 2313. Briefly, rats were anaesthetized with sodium pentobarbital (50 mg/kg, Sigma-Aldrich, MO, USA), followed by an injection of heparin (1,500 U/kg, APExBio, TX, USA) into the inferior vena cava and the introduction of a 14G catheter into the portal vein.…”

Effects of corilagin on alleviating cholestasis via farnesoid X receptor‐associated pathways in vitro and in vivo

“…LO2 cells were maintained in RPMI 1640 media supplemented with 10% FBS. As previously described (Ding et al, ), cells were distributed into either six‐well plates for real‐time PCR and Western blotting or 96‐well plates for the CCK8 experiments and preliminary lentiviral transfection experiments for 24 h. When the cell cultures reached 70% density, they were treated with corilagin, UDCA or DEX. After 24 h, the cells were harvested for real‐time PCR and Western blotting.…”

Corilagin ameliorates the extreme inflammatory status in sepsis through TLR4 signaling pathways

“…The procedure was followed by our past experiment [23, 24]. The expression of TLR-4 and associated signaling molecule proteins were detected by western-blot assay.…”