Preliminary study on fatal hyperthermia in rat liver tissue by Fourier transform infrared microspectroscopy

Wang, Zhijun; Tuo, Ya; Li, Bing; Deng, Kaifei; Han, Shun-qi; Luo, Yang; Sun, Qiran; Li, Zhengdong; Chen, Yijiu; Wang, Zhenyuan; Huang, Ping

doi:10.1080/00450618.2016.1156157

Cited by 5 publications

(2 citation statements)

References 37 publications

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“…Forty-seven male Sprague–Dawley rats weighing 260–300 g (provided by the Animal Centre of Xi’an Jiaotong University) were used for the experiment. The rat models were established as described previously [ 59 , 60 ]. Briefly, the rats were kept for one week in stainless steel cages at 23 ± 2°C until physical conditions were stabilized in a 12-h light/dark environment.…”

Section: Methodsmentioning

confidence: 99%

Biochemical detection of fatal hypothermia and hyperthermia in affected rat hypothalamus tissues by Fourier transform infrared spectroscopy

et al. 2019

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It is difficult to determinate the cause of death from exposure to fatal hypothermia and hyperthermia in forensic casework. Here, we present a state-of-the-art study that employs Fourier-transform infrared (FTIR) spectroscopy to investigate the hypothalamus tissues of fatal hypothermic, fatal hyperthermic and normothermic rats to determine forensically significant biomarkers related to fatal hypothermia and hyperthermia. Our results revealed that the spectral variations in the lipid, protein, carbohydrate and nucleic acid components are highly different for hypothalamuses after exposure to fatal hypothermic, fatal hyperthermic and normothermic conditions. In comparison with the normothermia group, the fatal hypothermia and hyperthermia groups contained higher total lipid amounts but were lower in unsaturated lipids. Additionally, their cell membranes were found to have less motional freedom. Among these three groups, the fatal hyperthermia group contained the lowest total proteins and carbohydrates and the highest aggregated and dysfunctional proteins, while the fatal hypothermia group contained the highest level of nucleic acids. In conclusion, this study demonstrates that FTIR spectroscopy has the potential to become a reliable method for the biochemical characterization of fatal hypothermia and hyperthermia hypothalamus tissues, and this could be used as a postmortem diagnostic feature in fatal hypothermia and hyperthermia deaths.

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Section: Methodsmentioning

confidence: 99%

Biochemical detection of fatal hypothermia and hyperthermia in affected rat hypothalamus tissues by Fourier transform infrared spectroscopy

et al. 2019

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“…Lin et al and Wu et al detected pulmonary edema fluid by FTIR imaging and constructed classification models for determining SCD, 41 anaphylactic shock, 42 hypothermia, 43 or diabetic ketoacidosis 44 as the cause. Tuo et al 45 and Wang et al 46 studied the vitreous fluid and liver tissue, respectively, of animal models of fatal hyperthermia and proposed FTIR spectroscopy as an alternative for determining the cause of death. These reports proved the application potential of FTIR spectroscopy in determining complex causes of death and inspired us to distinguish asphyxia from SCD as the cause of death.…”

Section: Introductionmentioning

confidence: 99%

Distinguishing Asphyxia from Sudden Cardiac Death as the Cause of Death from the Lung Tissues of Rats and Humans Using Fourier Transform Infrared Spectroscopy

et al. 2022

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The ability to determine asphyxia as a cause of death is important in forensic practice and helps us to judge whether a case is criminal. However, in some cases where the deceased has underlying heart disease, death by asphyxia cannot be determined by traditional autopsy and morphological observation under a microscope because there are no specific morphological features for either asphyxia or sudden cardiac death (SCD). Here, Fourier transform infrared (FTIR) spectroscopy was employed to distinguish asphyxia from SCD. A total of 40 lung tissues (collected at 0 h and 24 h postmortem) from 20 rats (10 died from asphyxia and 10 died from SCD) and 16 human lung tissues from 16 real cases were used for spectral data acquisition. After data preprocessing, 2675 spectra from rat lung tissues and 1526 spectra from human lung tissues were obtained for subsequent analysis. First, we found that there were biochemical differences in the rat lung tissues between the two causes of death by principal component analysis and partial least-squares discriminant analysis (PLS-DA), which were related to alterations in lipids, proteins, and nucleic acids. In addition, a PLS-DA classification model can be built to distinguish asphyxia from SCD. Second, based on the spectral data obtained from lung tissues allowed to decompose for 24 h, we could still distinguish asphyxia from SCD even when decomposition occurred in animal models. Nine important spectral features that contributed to the discrimination in the animal experiment were selected and further analyzed. Third, 7 of the 9 differential spectral features were also found to be significantly different in human lung tissues from 16 real cases. A support vector machine model was finally built by using the seven variables to distinguish asphyxia from SCD in the human samples. Compared with the linear PLS-DA model, its accuracy was significantly improved to 0.798, and the correct rate of determining the cause of death was 100%. This study shows the application potential of FTIR spectroscopy for exploring the subtle biochemical differences resulting from different death processes and determining the cause of death even after decomposition.

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Evaluating the effects of causes of death on postmortem interval estimation by ATR-FTIR spectroscopy

et al. 2019

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Preliminary study on fatal hyperthermia in rat liver tissue by Fourier transform infrared microspectroscopy

Cited by 5 publications

References 37 publications

Biochemical detection of fatal hypothermia and hyperthermia in affected rat hypothalamus tissues by Fourier transform infrared spectroscopy

Biochemical detection of fatal hypothermia and hyperthermia in affected rat hypothalamus tissues by Fourier transform infrared spectroscopy

Distinguishing Asphyxia from Sudden Cardiac Death as the Cause of Death from the Lung Tissues of Rats and Humans Using Fourier Transform Infrared Spectroscopy

Evaluating the effects of causes of death on postmortem interval estimation by ATR-FTIR spectroscopy

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