Preliminary study on local Indonesian sheep MC4R gene as a prospective marker for sustainable high production trait

Abstract

A prospective genetic marker to archive for sustainable growth traits of local Indonesian sheep is the Melanocortin-4 Receptor (MC4R) gene, which arrange feeding behavior. This study were examined the MC4R gene of Indonesian sheep for polymorphism and restriction enzyme for PCR-RFLP within the partial coding sequence (CDS) and 3’UTR regions. A 642 bp of PCR product was amplified from sixteen blood samples (Sakub: 2, Wonosobo: 3, Sapudi: 3, Garut:3, and Garut-Dorper cross: 2). Sequence alignments between the samples and GenBank (NC_056076) discovered five SNPs (CDS: SNPs 1039A>G and 1068G>C, 3’UTR region: SNPs 1403G>A, 1511C>A and 1526G>C). The SNP 1039A>G was categorized as a missense mutation (Met to Val). However, SNP 1068G>C was a silent mutation (Ala to Ala). The restriction enzyme analysis showed that SNPs 1039A>G and 1403G>A were recognized by BssSI and SfaNI. SNPs 1068G>C, 1511C>A and 1526G>C were not recognized by the enzyme. In conclusion, the MC4R gene of Indonesian has five SNPs discovered, among other two SNPs in CDS and three SNPs in 3’UTR region. BssSI and SfaNI enzymes are eligible for further genotyping of sheep samples using the PCR-RFLP method.