Preliminary study on Se-enriched Lentinula edodes mycelium as a proposal of new feed additive in selenium deficiency

Abstract

The presence of selenium in European soil is low and this causes its deficiency in livestock and, in consequence, in humans. This study aimed to obtain Lentinula (L.) edodes mycelium with the maximum content of selenium. This species was used for experiment based on its documented medicinal properties. Calves were fed with selenium-enriched L. edodes mycelium, and serum selenium concentration, average daily weight gains and selected immune parameters were estimated. The selenium-enriched mushroom was found to be safe based on cytotoxicity tests (MTT and LDH tests) and for this reason it was used for further experiments. The mean quantity of selenium in the serum of calves fed with selenium-enriched L. edodes mycelium was significantly higher than that of control calves. Additionally, the calves fed with selenium-enriched L. edodes mycelium had higher body weight gains than those of control calves. White blood cell counts and subpopulations of lymphocytes in the experimental and control calves were within the reference range. The administration of L. edodes enriched with selenium had a beneficial effect on state of health of the calves.

Pleurotus spp. Mycelia Enriched in Magnesium and Zinc Salts as a Potential Functional Food

“…Bioelement concentrations in fruiting bodies are markedly lower [ 36 ] and by means of comparison, P. ostreatus and P. eryngii fruiting bodies were obtained supplemented with zinc, containing 6 mg Zn per 100 g d.w. Poursaeid et al [ 37 ] obtained 189 mg Zn from 100 g d.w. of P. florida mycelium supplemented with 200 mg Zn/L medium. In another experimental work, Krakowska et al [ 21 ] using in vitro culture supplementation of another species of mushroom— A. bisporus —in inorganic zinc salts ZnSO 4 ·7H 2 O (200 mg/L medium) and magnesium MgSO 4 ·7H 2 O (2000 mg/L medium) also achieved higher adsorption efficiency: Mg—2696 mg/100 g d.w., Zn—446.2 mg/100 g d.w. A similar effect was observed in other experiments [ 38 ] where in vitro cultures L. edodes were conducted on substrates with the addition of ZnSO 4 (174.47 mg/L medium) and C 8 H 12 N 2 O 8 Zn—zinc hydrogen aspartate (200 mg/L medium)—in both cases, the addition of salt increased the amount of subsidized metal in the obtained biomass.…”

“…The lowest amount was obtained from in vitro cultures of P. citrinopileatus (6.4 mg/100 g d.w.), which were maintained on the medium enriched with ZnCl 2 salts. It should be noted that the quantities of Zn released into the artificial digestive juices of the Pleurotus species were much higher than those which Muszyńska et al [ 38 ] obtained for the species L. edodes (gastric juice—32.5 mg/100 g d.w. and intestinal juice—1.45 mg/100 g d.w.), research also carried out on a substrate enriched with inorganic zinc salt—ZnSO 4 . According to our results, P. pulmonarius released the highest quantity of Mg into the gastric juice (1280 mg/100 g d.w.), which was grown on medium enriched with MgSO 4 ·7H 2 O.…”

Are Obese Patients with Autism Spectrum Disorder More Likely to Be Selenium Deficient? Research Findings on Pre- and Post-Pubertal Children

“…Toenail samples were collected on the same day as the body fluid tests. The detailed procedures of serum, urine and nail sample collection, storage, preparation, and inductively coupled plasma mass spectrometry (ICP-MS) analysis were described in our previous papers [ 20 , 21 , 22 ].…”

A pilot study on the effect of a novel feed additive containing exogenous enzymes, acidifiers, sodium butyrate and silicon dioxide nanoparticles on selected cellular immune indices and body weight gains of calves

“…The tests were performed with an EPICS XL 4C flow cytometer (Beckman Coulter, Brea, CA, USA), using single-cell labelling according to Muszyńska et al . ( 24 ).…”