Preliminary Study to Investigate the Effect of Lactobacillus Reuteri Administration on Growth Performance, Immunological, Gut Microbiome and Intestinal Mucosa of Chicken

Abstract

The present study was conducted to investigate the efficacy of oral administration of L. reuteri on growth performance, intestine histomorphology, immunological and gut microbiome of broilers. A total of twenty healthy chickens were used in a five-week experimental trial. Birds were assigned into one of two groups with orally administrated L. reuteri probiotic and without probiotic-(Control -Phosphate-buffered saline). A significant (p<0.05) body weight gain was observed in the chickens in L. reuteri treatment group compare to those in the control group at the end of the trial. In addition, the serum IGF-1 cytokines level significantly enhanced in L. reuteri treatment group. However, there were no notable effects observed on the villus height, crypt depth, muscularis thickness, and submucosal thickness in chickens orally inject with and without L. reuteri. At the phylum level, the presence of Firmicutes (99.5%) was highly abundantin the L. reuteri treatment group. Moreover, the fecal microbial communities of Lactobacillus (99.9%) showed average relative abundance at genus level in L. reuteri treatment group. From this, we concluded that oral administration of L. reuteri would be beneficial to enhance the body weight gain, gut microbiome, and immune status of broiler.

Mortality, growth, and egg production do not differ between nontransgenic and transgenic female chickens with ubiquitous expression of the 3D8 single chain variable fragment gene

“…In feeding studies by Sureshkumar et al (2019 , 2020 ), the serum level of IGF-1 was decreased, and growth performance was improved. However, the level of IGF-1 was not decreased or increased in groups fed WT L. salivarius and L. reuteri ( Sureshkumar et al, 2021 , 2022 ). In the current study, TG chickens showed reduced serum levels of IGF-1 .…”