Preliminary X-ray diffraction studies of an RNA pseudoknot that inhibits HIV-1 reverse transcriptase

Abstract

Crystals of a 26-nucleotide pseudoknot RNA, PK26, have been grown. The RNA was produced using phosphoramidite chemistry and was purified by denaturing polyacrylamide electrophoresis. The crystallization was robust with respect to changes in the number of nucleotides and to the salt used as precipitant. The crystals belong to space group P4122 or P4322 with unit-cell dimensions a=b=61.6, c=98.9A. The best crystals diffract X-rays to 2.9,~. Three different sequences incorporating a single 5-bromo-deoxyuridine or 5-bromouridine nucleotide were also crystallized. Two of these derivatives are being used to determine the structure by multiple isomorphous replacement.