Preosteoblasts and fibroblasts respond differently to anatase titanium dioxide nanoparticles: A cytotoxicity and inflammation study

Bernier, Marie-Charlotte; Kirat, Karim El; Besse, Marie; Morandat, Sandrine; Vayssade, Muriel

doi:10.1016/j.colsurfb.2011.09.044

Cited by 28 publications

(26 citation statements)

References 38 publications

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“…Concerning to anatase nanoparticles effect on MC-3T3 cells, increased cell granularity and DNA fragmentation have been observed. In addition, those nanoparticles induced higher secretion of the pro-inflammatory cytokine (IL-6) known to mediate osteolysis 36 . Nonetheless, rutile debris seems to possess a lower bio reactivity on osteoblasts with a reduced release of inflammatory factors 37 .…”

Section: Discussionmentioning

confidence: 99%

Trojan-Like Internalization of Anatase Titanium Dioxide Nanoparticles by Human Osteoblast Cells

Ribeiro

Gemini-Piperni

Travassos

et al. 2016

Sci Rep

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Dentistry and orthopedics are undergoing a revolution in order to provide more reliable, comfortable and long-lasting implants to patients. Titanium (Ti) and titanium alloys have been used in dental implants and total hip arthroplasty due to their excellent biocompatibility. However, Ti-based implants in human body suffer surface degradation (corrosion and wear) resulting in the release of metallic ions and solid wear debris (mainly titanium dioxide) leading to peri-implant inflammatory reactions. Unfortunately, our current understanding of the biological interactions with titanium dioxide nanoparticles is still very limited. Taking this into consideration, this study focuses on the internalization of titanium dioxide nanoparticles on primary bone cells, exploring the events occurring at the nano-bio interface. For the first time, we report the selective binding of calcium (Ca), phosphorous (P) and proteins from cell culture medium to anatase nanoparticles that are extremely important for nanoparticle internalization and bone cells survival. In the intricate biological environment, anatase nanoparticles form bio-complexes (mixture of proteins and ions) which act as a kind of ‘Trojan-horse’ internalization by cells. Furthermore, anatase nanoparticles-induced modifications on cell behavior (viability and internalization) could be understand in detail. The results presented in this report can inspire new strategies for the use of titanium dioxide nanoparticles in several regeneration therapies.

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Section: Discussionmentioning

confidence: 99%

Trojan-Like Internalization of Anatase Titanium Dioxide Nanoparticles by Human Osteoblast Cells

Ribeiro

Gemini-Piperni

Travassos

et al. 2016

Sci Rep

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“… 45 , 50 , 51 , 58–61 Mitochondrial and cell membrane permeability both increase in the presence of TiO 2 particles, suggesting significant cell stress. 58 , 62 Investigations involving human MSCs, osteoclasts, and histiocytic lymphoma cells have confirmed similar responses, 41 , 60 , 63 , 64 yet the current challenge is to define how these cells interact with other peri-implant cell types such as preosteoblasts and osteoblasts.…”

Section: Cellular Uptake and Cytotoxicity Of Tio 2 mentioning

confidence: 93%

Local Cellular Responses to Titanium Dioxide from Orthopedic Implants

Yao

Lewallen

Trousdale

et al. 2017

BioResearch Open Access

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We evaluated recently published articles relevant to the biological effects of titanium dioxide (TiO2) particles on local endogenous cells required for normal bone homeostasis, repair, and implant osseointegration. Structural characteristics, size, stability, and agglomeration of TiO2 particles alter the viability and behavior of multiple bone-related cell types. Resulting shifts in bone homeostasis may increase bone resorption and lead to clinical incidents of osteolysis, implant loosening, and joint pain. TiO2 particles that enter cells (through endocytosis or Trojan horse mechanism) may further disrupt implant retention. We propose that cellular responses to titanium-based nanoparticles contribute to pathological mechanisms underlying the aseptic loosening of titanium-based metal implants.

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“…Although the engineering of NPs internalization into cells is considered as non-specific according to studies with microcopy images 38 , endocytosis is an important mechanism to Ag 39 and TiO2 nanoparticles 40 cell intake. However, particle agglomeration can mitigate the toxic effects of particle size, which may vary depending on cell type and internalization mechanisms 41,42 . In this study, micrographs and granulometric analysis show that AgNP_SiO2 and TiO2 present a tendency to agglomerate, with a set size of 12.97 µm and 0.29 µm, respectively.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Toxicity of Silver/Silica and Titanium Dioxide Particles in Mammalian Cells

Pittol

Tomacheski

Simões

et al. 2018

Braz. arch. biol. technol.

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The increasing concern over the spread of diseases has lead to a high consumption of antimicrobial additives in the medical and industrial fields. Since these particles can lixiviate from loaded materials, the contact between this additive and mammalian cells can occur during manufacture, use and disposal of the products. Silver on fumed silica (AgNP_SiO2) and titanium dioxide (TiO2) can be used as antimicrobial additives that are applied in polymeric formulation. While these additives can inhibit bacteria, fungus and virus proliferation; they may also be harmful to humans. Standard toxicological studies were undertaken using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide), CBPI (cytokinesis-block proliferation index) and micronucleus assay using different sets of additive concentrations. The nanosize of the samples evaluated was confirmed by transmission electronic microscopy. No significant micronucleus frequency increase or cell viability reduction were observed with the exposure of L-929 murine fibroblast cells to AgNP_SiO2 and TiO2 particles at any of the tested concentrations. The non toxic effect of the analyzed particles can be explained by considering its agglomeration tendency, composition, and crystalline form. Further investigations should be done to understand the interference of agglomeration and how it affects the toxicological study.

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Preosteoblasts and fibroblasts respond differently to anatase titanium dioxide nanoparticles: A cytotoxicity and inflammation study

Cited by 28 publications

References 38 publications

Trojan-Like Internalization of Anatase Titanium Dioxide Nanoparticles by Human Osteoblast Cells

Trojan-Like Internalization of Anatase Titanium Dioxide Nanoparticles by Human Osteoblast Cells

Local Cellular Responses to Titanium Dioxide from Orthopedic Implants

Evaluation of the Toxicity of Silver/Silica and Titanium Dioxide Particles in Mammalian Cells

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