1973
DOI: 10.1021/bi00745a038
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Preparation and active-site specific properties of sturgeon muscle glyceraldehyde-3-phosphate dehydrogenase

Abstract: Sturgeon muscle glyceraldehyde-3-phosphate de-hydrogenase has been isolated and purified to maximal activity. The purified enzyme contains four unusually reactive cysteine sulfhydryls per 145,000 daltons. This highly selective

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Cited by 107 publications
(72 citation statements)
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“…The absorbance ratio A280/A260 and absorption coefficient as a function of the clac3PdAD+ content covalently bound to glyceraldehyde-3-phosphate dehydrogenase from sturgeon and B. stearothermophilus (results not shown) were similar to those obtained by Seydoux et al [7] with NAD' on the sturgeon enzyme.…”
Section: Stoichiometry Of the Alkylationsupporting
confidence: 86%
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“…The absorbance ratio A280/A260 and absorption coefficient as a function of the clac3PdAD+ content covalently bound to glyceraldehyde-3-phosphate dehydrogenase from sturgeon and B. stearothermophilus (results not shown) were similar to those obtained by Seydoux et al [7] with NAD' on the sturgeon enzyme.…”
Section: Stoichiometry Of the Alkylationsupporting
confidence: 86%
“…Sturgeon glyceraldehyde-3-phosphate dehydrogenase was prepared using the procedure described by Seydoux et al [7] up to the ammonium sulfate fractionation. At this stage, the protein solution (70 ml, corresponding to 100 g of sturgeon muscle obtained from Caviar Volga, Paris) was dialyzed against 5 mM EDTA, 1 mM dithiothreitol, pH 6.6 and then applied to a column of CM-cellulose (CM 52, Whatman column, 2 x 20 cm), equilibrated with 5 mM EDTA (pH 6.6).…”
Section: Enzyme Purificationmentioning
confidence: 99%
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“…GPD was prepared from sturgeon muscle by the method in [7] . Protein concentration was determined from A280 using E280 = 1.29 X 10' M-' cm-'.…”
Section: Isolation Of Gpdmentioning
confidence: 99%