In order to solve the problem of road dust pollution, an ecological dust suppressant for road surfaces has been developed using monomer, orthogonal, and optimization experiments and based on the dust raising mechanism. A humectant, hygroscopic agent, coagulant and surfactant and their concentration ranges have been determined through monomer experiment. The preliminary formula of the dust suppressant has been obtained through orthogonal experiment, with the water loss rate, moisture content rate, viscosity value, and surface tension value serving as experimental indexes. The optimal formula for the dust suppressor has been calculated through an optimization experiment, with the toxicity, moisture absorption and retention performance of plants, and the relative damage rate of plant seeds serving as experimental indexes. Based on the performance characterization of ecological road dust suppressant, the ecologically and environmentally friendly dust suppressant demonstrates fine moisture absorption and retention performance, good wind and rain erosion resistance, and no toxicity. The ecological road dust suppressant developed herein covers extensive raw material sources. It is ecologically and environmentally friendly, fit for most urban roads, and has a fine dust suppression effect. Meanwhile, it also can bring in good economic and social benefits, demonstrating its broad application prospects.