Preparation and characterization of amnion hydrogel and its synergistic effect with adipose derived stem cells towards IL1β activated chondrocytes

Bhattacharjee, Maumita; Ivirico, Jorge L. Escobar; Kan, Ho-Man; Bordett, Rosalie; Pandey, Rishikesh; Otsuka, Takayoshi; Nair, Lakshmi S.; Laurencin, Cato T.

doi:10.1038/s41598-020-75921-w

Cited by 31 publications

(37 citation statements)

References 53 publications

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“…An increase in PRG4 upon IL-1β–induced inflammation was observed and may indicate a negative feedback regulation of inflammation. Interestingly, in addition to having a similar antiinflammatory effect compared to ADSCs [reducing inflammatory effects ADAMTS5 ( 49 , 50 )], SASC was able to reverse the effects of inflammation on NO, SOX9, and PRG4 ( Fig. 4 A – D ).…”

Section: Discussionmentioning

confidence: 93%

“…ADSCs were isolated and purified as described by Bhattacharjee et al. ( 49 ). Rats were killed with CO 2 asphyxiation followed by cervical dislocation.…”

Section: Methodsmentioning

confidence: 99%

“…ADSCs were isolated from 6-to 8-wk-old Sprague-Dawley rats in accordance with the experimental guidelines and regulations approved by University of Connecticut Health Center Institutional Animal Care and Use Committee-approved protocol. ADSCs were isolated and purified as described by Bhattacharjee et al (49). Rats were killed with CO 2 asphyxiation followed by cervical dislocation.…”

Section: Semmentioning

confidence: 99%

“…qRT-PCR. qRT-PCR was done using a procedure used in our group (49). Briefly, total RNA was isolated from cells pooled from four wells using the RNeasy Mini Kit (74104; Qiagen) according to the manufacturer's instructions.…”

Section: Semmentioning

confidence: 99%

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The synthetic artificial stem cell (SASC): Shifting the paradigm of cell therapy in regenerative engineering

Shah

Esdaille

Bhattacharjee

et al. 2022

Proc. Natl. Acad. Sci. U.S.A.

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Stem cells are of great interest in tissue regeneration due to their ability to modulate the local microenvironment by secreting bioactive factors (collectively, secretome). However, secretome delivery through conditioned media still requires time-consuming cell isolation and maintenance and also may contain factors antagonistic to targeted tissue regeneration. We have therefore engineered a synthetic artificial stem cell (SASC) system which mimics the paracrine effect of the stem cell secretome and provides tailorability of the composition for targeted tissue regeneration. We report the first of many applications of the SASC system we have formulated to treat osteoarthritis (OA). Choosing growth factors important to chondrogenesis and encapsulating respective recombinant proteins in poly (lactic-coglycolic acid) 85:15 (PLGA) we fabricated the SASC system. We compared the antiinflammatory and chondroprotective effects of SASC to that of adipose-derived stem cells (ADSCs) using in vitro interleukin 1B-induced and in vivo collagenase-induced osteoarthritis rodent models. We have designed SASC as an injectable therapy with controlled release of the formulated secretome. In vitro, SASC showed significant antiinflammatory and chondroprotective effects as seen by the up-regulation of SOX9 and reduction of nitric oxide, ADAMTS5, and PRG4 genes compared to ADSCs. In vivo, treatment with SASC and ADSCs significantly attenuated cartilage degeneration and improved the biomechanical properties of the articular cartilage in comparison to OA control. This SASC system demonstrates the feasibility of developing a completely synthetic, tailorable stem cell secretome which reinforces the possibility of developing a new therapeutic strategy that provides better control over targeted tissue engineering applications.

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Section: Discussionmentioning

confidence: 93%

“…ADSCs were isolated and purified as described by Bhattacharjee et al. ( 49 ). Rats were killed with CO 2 asphyxiation followed by cervical dislocation.…”

Section: Methodsmentioning

confidence: 99%

Section: Semmentioning

confidence: 99%

Section: Semmentioning

confidence: 99%

See 2 more Smart Citations

The synthetic artificial stem cell (SASC): Shifting the paradigm of cell therapy in regenerative engineering

Shah

Esdaille

Bhattacharjee

et al. 2022

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

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“…Lastly, with advancements towards 3-dimensional cell printing [55,[64][65][66], it would be interesting to evaluate the effect of printing the SVF-like extracellular matrix in a hydrogel scaffold for osteochondral defects, or incorporating SVF into an injectable hydrogel for enhanced retention and viability in the local environment as has been demonstrated in previous studies using adipose-derived stem cells [67].…”

Section: Future Directionsmentioning

confidence: 99%

Stromal Vascular Fraction for Osteoarthritis of the Knee Regenerative Engineering

Ude

Shah

Ogueri

et al. 2021

Regen. Eng. Transl. Med.

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Purpose The knee joint is prone to osteoarthritis (OA) due to its anatomical position, and several reports have implicated the imbalance between catabolic and anabolic processes within the joint as the main culprit, thus leading to investigations towards attenuation of these inflammatory signals for OA treatment. In this review, we have explored clinical evidence supporting the use of stromal vascular fraction (SVF), known for its anti-inflammatory characteristics for the treatment of OA. Methods Searches were made on PubMed, PMC, and Google Scholar with the keywords “adipose fraction knee regeneration, and stromal vascular fraction knee regeneration, and limiting searches within 2017–2020. Results Frequently found interventions include cultured adipose-derived stem cells (ADSCs), SVF, and the micronized/microfragmented adipose tissue-stromal vascular fraction (MAT-SVF). Clinical data reported that joints treated with SVF provided a better quality of life to patients. Currently, MAT-SVF obtained and administered at the point of care is approved by the Food and Drug Administration (FDA), but more studies including manufacturing validation, safety, and proof of pharmacological activity are needed for SVF. The mechanism of action of MAT-SVF is also not fully understood. However, the current hypothesis indicates a direct adherence and integration with the degenerative host tissue, and/or trophic effects resulting from the secretome of constituent cells. Conclusion Our review of the literature on stromal vascular fraction and related therapy use has found evidence of efficacy in results. More research and clinical patient follow-up are needed to determine the proper place of these therapies in the treatment of osteoarthritis of the knee. Lay Summary Reports have implicated the increased inflammatory proteins within the joints as the main cause of osteoarthritis (OA). This has attracted interest towards addressing these inflammatory proteins as a way of treatment for OA. The concentrated cell-packed portion of the adipose product stromal vascular fraction (SVF) from liposuction or other methods possesses anti-inflammatory effects and has been acclaimed to heal OA. Thus, we searched for clinical evidence supporting their use, for OA treatment through examining the literature. Data from various hospitals support that joints treated with SVF provided a better quality of life to patients. Currently, there is at least one version of these products that are obtained and given back to patients during a single clinic visit, approved by the FDA.

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ADSCC‐CM‐Induced Keratin Hydrogel‐Based Bioactive Microneedle Patch Containing Triamcinolone Acetonide for the Treatment of Pathological Scar

Li,

Yi,

et al. 2024

Adv Funct Materials

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Pathological scars (PS) are involved in the excessive response of inflammation and overactivation of myofibroblasts. Herein, a novel microneedle (MN) patch based on the adipose‐derived stem cell concentrated conditioned medium (ADSCC‐CM) cross‐linked keratin hydrogel is developed to load triamcinolone acetonide (TA), thereby achieving the dual‐drug delivery of ADSCC‐CM and TA to simultaneously reduce the inflammation and guide myofibroblast behaviors. Results not only confirm the ability of ADSCC‐CM to drive the formation of keratin‐based hydrogel, but also verify the dual‐drug release capacities of the hydrogel‐developed MNs (TA@AC‐MN). Using human hyperplastic scar fibroblast (HSF), the combination of ADSCC‐CM and TA demonstrates a pronounced synergistic effect in mitigating the detrimental effects of the inflammatory microenvironment on HSFs, including suppressing the production of reactive oxygen species and attenuating the expression of inflammatory factors. Compared with the clinically used TA, TA@AC‐MN promotes scar biomimetic repair (i.e., optimizing the proportion of collagen and increasing the tissue tensile strength). This study demonstrates that TA@AC‐MN has the capacity to provide a self‐managing and minimally invasive therapeutic strategy for PS.

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Preparation and characterization of amnion hydrogel and its synergistic effect with adipose derived stem cells towards IL1β activated chondrocytes

Cited by 31 publications

References 53 publications

The synthetic artificial stem cell (SASC): Shifting the paradigm of cell therapy in regenerative engineering

The synthetic artificial stem cell (SASC): Shifting the paradigm of cell therapy in regenerative engineering

Stromal Vascular Fraction for Osteoarthritis of the Knee Regenerative Engineering

ADSCC‐CM‐Induced Keratin Hydrogel‐Based Bioactive Microneedle Patch Containing Triamcinolone Acetonide for the Treatment of Pathological Scar

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