Preparation and characterization of branched polymers as postoperative anti-adhesion barriers

Way, Tzong‐Der; Hsieh, Shih‐Rong; Chang, Chi‐Jung; Hung, Tsung-Wei; Chiu, Chun-Hwei

doi:10.1016/j.apsusc.2009.12.029

Cited by 17 publications

(8 citation statements)

References 17 publications

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“…Sulfuric acid 2 N (H 2 SO 4 ) was obtained from Panreac (Barcelona, Spain). The primary antibodies used to detect FN (human polyclonal anti‐human FN4) were also purchased from Sigma (Spain); the RGD (monoclonal antibody anti‐human FN, cellular union domain, clone P1H114) was from Chemicon (Spain), and FNIII 7–10 (HFN7.1 antibody5) was from Abcam (Spain). The secondary antibodies (conjugated with horse radish peroxidase [HRP]) rabbit polyclonal secondary antibody to mouse IgG ‐ H&L (HRP) was obtained from Abcam (Spain) and anti‐rabbit IgG–peroxidase antibody produced in goat from Sigma (Spain).…”

Section: Methodsmentioning

confidence: 99%

“…In many instances, cells adhere to synthetic surfaces via proteins adsorbed from physiological fluids and culture media. The physicochemical properties of the material, including topography, chemistry, and surface energy, modulate protein adsorption; this fact plays an important role in determining the design of the biomaterial 1–6. The amount and conformation of the adsorbed proteins affect different cellular functions, including adhesion, spreading, migration, and differentiation.…”

Section: Introductionmentioning

confidence: 99%

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Fibronectin fixation on poly(ethyl acrylate)–based copolymers

et al. 2013

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2 AbstractThe aim of this paper is to quantify the adhered fibronectin (by adsorption and/or grafting) and the exposure of its cell adhesive motifs (RGD and FNIII7-10) on poly (ethyl acrylate) copolymers whose chemical composition has been designed to increase wettability and to introduce acid functional groups. Fibronectin was adsorbed to poly (ethyl acrylate), poly (ethyl acrylate-co-hydroxyethyl acrylate), poly (ethyl acrylate-co-acrylic acid) and poly (ethyl acrylate-co-methacrylic acid) copolymers and covalently cross-linked to poly (ethyl acrylateco-acrylic acid) and poly (ethyl acrylate-co-methacrylic acid) copolymers. Amount of adhered fibronectin and exhibition of RGD and FNIII7-10 fragments involved in cell adhesion were quantified with ELISA tests.Even copolymers with a lower content of the hydrophilic component showed a decrease in water contact angle. In addition, fibronectin was successfully fixed on all surfaces, especially on the hydrophobic surfaces. However, it was demonstrated that exposure of its cell adhesion sequences, which is the key factor in cell adhesion and proliferation, was higher for hydrophilic surfaces.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fibronectin fixation on poly(ethyl acrylate)–based copolymers

et al. 2013

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“…Nevertheless, it is found that these materials do not achieve a perfect efficacy from clinical perspective [10]. For instance, the biodegradation rate of hyaluronic acid is too fast in the damage location, which will greatly influence the result of anti-adhesion [11]. Since hydroxyapatite cannot be absorbed by the body, it will exist doi: 10.1007/s11426-015-5425-7 for a long time in the body and may cause some mechanical irritation [9].…”

Section: Introductionmentioning

confidence: 95%

A comparative study of preventing postoperative tendon adhesion using electrospun polyester membranes with different degradation kinetics

et al. 2015

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Complications arising from tendon injury include tendon sheath infection and peritendinous adhesion, in which tendon adhesion often leads to serious motor dysfunction. In this work, the electrospun membranes of poly(L-lactide) (PLA) and poly(-caprolactone) (PCL) with different degradation kinetics were used to investigate their efficacy for anti-adhesion toward Achilles tendon repair. Compared with the PCL membrane, the PLA sample showed a faster rate of degradation in 42 d, and all the degradation media (i.e., phosphate-buffered saline) maintained at a constant pH of around 7.4. Meanwhile, the superior biocompatibility of both the PLA and PCL membranes were proved by the in vitro cellular adhesion tests and in vivo histopathological assays. Simultaneously, the PLA membrane was more effective than the PCL sample in decreasing adhesion and promoting functional recovery. Furthermore, the experiment result was further confirmed by hematoxylin-eosin and Masson's trichrome staining, and type I collagen immunohistochemical analysis. All results revealed that the model treated with the electrospun PLA membrane was obviously better with regard to both anti-adhesion and tendon repair than that in the PCL membrane group. Considering the results of degradation and adhesion prevention efficacy, the electrospun polyester membranes, especially the PLA one, would be applied with fascinating potential in clinical prevention of postoperative tendon adhesion.anti-adhesion, Achilles tendon repair, biodegradability, electrospun membrane, poly(L-lactide), poly(-caprolactone)

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“…Several limitations still exist that include selective blockage of scar tissue formation, appropriate degradation profile, and overall clinical efficacy. Cross‐linked hyaluronic acid based materials, currently the most commonly used material for preventing peritendinous adhesions, tend to degrade too quickly to be used for tendon surgery . Many synthetic materials such as poly(lactic‐co‐glycolic acid), however, produce noxious acidic by‐products during degradation …”

Section: Introductionmentioning

confidence: 99%

“…Cross-linked hyaluronic acid based materials, currently the most commonly used material for preventing peritendinous adhesions, tend to degrade too quickly to be used for tendon surgery. 10 Many synthetic materials such as poly(lactic-co-glycolic acid), however, produce noxious acidic by-products during degradation. 11 Cartilage extracellular matrix (ECM) contains antiadhesive and antiangiogenic molecules such as chondromodulin-1, thrombospondin-1, and endostatin.…”

mentioning

confidence: 99%

Cross‐linked cartilage acellular matrix film decreases postsurgical peritendinous adhesions

et al. 2019

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Cartilage extracellular matrix contains antiadhesive and antiangiogenic molecules such as chondromodulin‐1, thrombospondin‐1, and endostatin. We have aimed to develop a cross‐linked cartilage acellular matrix (CAM) barrier for peritendinous adhesion prevention. CAM film was fabricated using decellularized porcine cartilage tissue powder and chemical cross‐linking. Biochemical analysis of the film showed retention of collagen and glycosaminoglycans after the fabrication process. Physical characterization of the film showed denser collagen microstructure, increased water contact angle, and higher tensile strength after cross‐linking. The degradation time in vivo was 14 d after cross‐linking. The film extract and film surface showed similar cell proliferation, while inhibiting cell migration and cell adhesion compared to standard media and culture plate, respectively. Application of the film after repair resulted in similar tendon healing and significantly less peritendinous adhesions in a rabbit Achilles tendon injury model compared to repair only group, demonstrated by histology, ultrasonography, and biomechanical testing. In conclusion, the current study developed a CAM film having biological properties of antiadhesion, together with biomechanical properties and degradation profile suitable for prevention of peritendinous adhesions.

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Preparation and characterization of branched polymers as postoperative anti-adhesion barriers

Cited by 17 publications

References 17 publications

Fibronectin fixation on poly(ethyl acrylate)–based copolymers

Fibronectin fixation on poly(ethyl acrylate)–based copolymers

A comparative study of preventing postoperative tendon adhesion using electrospun polyester membranes with different degradation kinetics

Cross‐linked cartilage acellular matrix film decreases postsurgical peritendinous adhesions

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