Preparation and Characterization of Ceftriaxone Sodium Encapsulated Chitosan Nanoparticles

Manimekalai, P.; Dhanalakshmi, R.; Manavalan, R.

doi:10.22159/ijap.2017v9i6.16317

Cited by 24 publications

(30 citation statements)

References 16 publications

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“…Suitable dilution of A-CNPs dispersion (10 times) was prepared for estimating the particle size (PS), polydispersity index (PDI) and zeta potential (ZP) in triplicate on Zetasizer (Malvern Instruments, U. K.) by using dynamic light scattering technique. The scattering was recorded at 90 ° angle and 25 °C temperature [11,12]. TEM was performed for examining the surface morphology and PS of formulated A-CNPs by using High-resolution transmission electron microscope (TECNAI, at 200 kV).…”

Section: Ps Pdi and Zpmentioning

confidence: 99%

Formulation and Evaluation of Allopurinol Loaded Chitosan Nanoparticles

2019

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Objective: The objective of the present investigation was to fabricate and characterize allopurinol loaded chitosan nanoparticles (A-CNPs) for sustained release of drug. Methods: The allopurinol loaded chitosan nanoparticles were successfully prepared by employing the ionotropic gelation method. Further, particle size (PS), polydispersity index (PDI), zeta potential (ZP), Differential Scanning Calorimetry (DSC), entrapment efficiency (EE), Transmission Electron Microscopy (TEM), in vitro drug release, X-Ray Diffraction (XRD) and Fourier transform infrared (FTIR) were used for evaluating formulated A-CNPs Results: A-CNPs was successfully prepared and the particle size, polydispersity index, ZP and entrapment efficiency were found to be 375.3±10.1 nm, 0.362±0.01 and 32.5±2.7 mV and 52.56±0.10% respectively. In vitro release profile of A-CNPs showed sustained release and Higuchi model was found to be best fit for drug release kinetics. FTIR study depicted no chemical interaction between pure drug allopurinol (AL) and other excipients. Conclusion: The sustained release formulation of allopurinol was successfully prepared using HMW chitosan and evaluated for different parameters.

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Section: Ps Pdi and Zpmentioning

confidence: 99%

Formulation and Evaluation of Allopurinol Loaded Chitosan Nanoparticles

2019

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“…The formed suspension was centrifuged (Thermo scientific, Germany) for 30 min at 10000 rpm. The nanoparticles were repeatedly washed with deionized water and freeze-dried for 48h at −80 °C to obtain the powdered nanoparticles [24,25].…”

Section: Preparation Of Cs Npsmentioning

confidence: 99%

Preparation of Chitosan-TPP Nanoparticles: The Influence of Chitosan Polymeric Properties and Formulation Variables

Al-Nemrawi

Alsharif

Dave³

2018

Int J App Pharm

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Objective: The aim of this work was to prepare chitosan nanoparticles (CS NPs) using sodium tripolyphosphate (TPP) as crosslinker and to study the effect of chitosan polymeric properties and experimental conditions on the properties and stability of NPs.Methods: CS NPs were prepared by ionic gelation method, using TPP as a crosslinker. The particle size (PS), polydispersity index (PDI), zeta potential (ZP) and the morphologies of the NPs were studied. CS NPs prepared by varying the concentration of TPP, Chitosan molecular weight and its degree of deacetylation, the stirring speed, the rate of TPP addition and the freeze-drying method to study the effect of these variables on the NPs. The stability of the CS NPs was evaluated by storing aqueous suspensions of NPs and comparing the PS, PDI and ZP at the beginning and the end of the experiment.Results: This study shows that the PS, ZP and dispersity of the NPs depend on the chitosan polymeric properties and experimental conditions. The NPs sizes range between 145.73 and 724.23 nm. They all carried positive charges ranging between+4.32 and+43.67 mV. Most of the NPs have the same sizes after freeze-drying, but showed higher monodispersity and ZP, indicating higher stability. After twenty days of studying the stability, the NPs that had low ZP showed a large increment in size in comparison to the highly charged NPs.Conclusion: In conclusion, the polymeric properties and formulation variables in the ionic gelation method have a great influence on the CS NPs formed.

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“…The amino group in chitosan has a pKa value of ∼6.5, thus, chitosan is positively charged and soluble in acidic to a neutral solution. Ionic gelation method has been extensively used in the preparation of chitosan nanoparticles as it has numerous advantages such as preparation of small size and compact structured particles [21], use of aqueous media [22], and control of colloidal characteristics of the nanoparticles by the variation of formulation and process parameters [23]. The method involves ionic cross-linking between cations of chitosan and anion such as sodium tripolyphosphate (TPP) [24].…”

Section: Introductionmentioning

confidence: 99%

Self-Assembled Chitosan Nanoparticles for Percutaneous Delivery of Caffeine: Preparation, Characterization and in Vitro Release Studies

et al. 2018

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Objective: Chitosan (CS)-tripolyphosphate (TPP)-nanoparticles (NPs) have been extensively studied during the past few decades due to their wellrecognized applicability in various fields. The present study attempts to optimise the development of these nanoparticles to enhance the percutaneous delivery of caffeine.Methods: CS-TPP-NPs were prepared via ionic cross-linking of CS and TPP and were characterized. The influence of several formulation conditions (CS: TPP mass ratio and concentration of caffeine) and process parameters (stirring speed, stirring time and ultra-sonication time) on the colloidal characteristics of CS-TPP-NPs were investigated and the resulting nanoparticles were characterized using scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) and x-ray diffraction (XRD) analyses. Physicochemical properties, including particle size, zeta potential and polydispersity index (PDI) were examined, and in vitro release studies were conducted to ascertain the release profile of caffeine from the nanoparticles. In addition, the colloidal stability of the prepared NPs was also assessed on storage.Results: Process parameters appeared to exert a significant effect on the physicochemical characteristics of the CS-TPP-NPs. The CS-TPP-NPs prepared under optimum conditions (CS concentration of 0.2 mg/ml, CS: TPP volume ratio of 25:12 ml, stirred at 700 rpm for 60 min, with 0.97 mg/ml caffeine concentration and treatment with low ultra-sonication for 30 min) had shown a mean particle size of ~143.43±1.69 nm, zeta potential of+43.13±1.10 mV, PDI of ~0.30±0.01. A drug loading capacity and encapsulation efficiency of 48.89% and 60.69%, respectively, were obtained. Cumulative release study for drug-loaded CS-NPs was significantly (p<0.001, paired t-test) higher (58.7% caffeine released) compared to control formulation (41.5% caffeine released) after 72 h. Stability studies conducted for 28 d showed that caffeine-loaded CS-NPs degraded much quicker when stored at 25 ⁰C than 4 ⁰C. It was also noted that caffeine-loaded CS-NPs in the freeze-dried form were unstable as the surface charge of nanoparticles dropped from positive zeta potential to-3.55 mV within 2 d at 4 ⁰C and at 25 ⁰C, surface charge dropped to-3.16 mV within 14 d of the experiment. Conclusion:Chitosan (CS)-tripolyphosphate (TPP)-nanoparticles (NPs) appear to be a promising strategy to achieve sustained percutaneous delivery of caffeine.

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Preparation and Characterization of Ceftriaxone Sodium Encapsulated Chitosan Nanoparticles

Cited by 24 publications

References 16 publications

Formulation and Evaluation of Allopurinol Loaded Chitosan Nanoparticles

Formulation and Evaluation of Allopurinol Loaded Chitosan Nanoparticles

Preparation of Chitosan-TPP Nanoparticles: The Influence of Chitosan Polymeric Properties and Formulation Variables

Self-Assembled Chitosan Nanoparticles for Percutaneous Delivery of Caffeine: Preparation, Characterization and in Vitro Release Studies

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