Preparation and characterization of polyclonal and monoclonal antibodies to polyamines

Schipper, Raymond G.; Jonis, J.A.; Rutten, R.G.J.; Tesser, G. I.; Verhofstad, A.A.J.

doi:10.1016/0022-1759(91)90245-b

Cited by 17 publications

(4 citation statements)

References 10 publications

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“…The observed cross-reactivity of the anti-Spm antibod ies with Spd is in line with earlier findings with polyclonal [13.14] and monoclonal [15,16] anti-Spm antibodies and is probably explained by the very close structural similarity between a tetramine (Spm) and a triamine (Spd).…”

Section: Discussionsupporting

confidence: 79%

“…It has been claimed that, because of their low molecular weight, polyamines behave like haptens and require cova lent conjugation to a carrier in order to be immunogenic [13][14][15][16], Here, we present evidence that antibodies to poly amines can be obtained by immunization with nonchemically coupled complexes of Spm and Spd with OVA, as well as with Spm in the free form. However, we cannot rule out the possibility that free polyamines might be complexed in vivo to peptides or proteins and become immu nogenic as a result.…”

Section: Discussionmentioning

confidence: 72%

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Immunization of Rabbits with Spermine Induces Antibodies to Self Antigens

Delcros

Muller

et al. 1993

Int Arch Allergy Immunol

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Rabbits were immunized under different schedules with spermine in the free form or with random noncovalent complexes of spermine or spermidine with ovalbumin. The specificity of the induced antibodies was determined by ELISA and by dot-immunobinding assay. Our results show that in vitro conjugation of spermine and spermidine to a carrier is not an obligatory prerequisite for obtaining corresponding antibodies. Anti-spermine antibodies were found in 9 of 19 animals injected with spermine. Furthermore, all 19 rabbits produced distinct populations of IgM and IgG antibodies which reacted with histones, various synthetic peptides of histones, as well with ubiquitin, a peptide of ubiquitin, dsDNA and two 29-base 5′ synthetic oligodesoxynucleotides. Even in antisera with no detectable reactivity with spermine, antibodies to some of the unrelated antigens were found. The pattern of reactivity of the antisera with the various antigens was different for each immunized animal. These findings lend support to the view that polyamines may play a role in the appearance of autoantibodies.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 72%

Immunization of Rabbits with Spermine Induces Antibodies to Self Antigens

Delcros

Muller

et al. 1993

Int Arch Allergy Immunol

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“…Therefore, considerable effort was put into the immunocytochemical detection of polyamines. Polyclonal (Quash and Wilson, 1967; Bartos et al, 1975; Bartos and Bartos, 1978; Fujiwara et al, 1983; Schipper et al, 1991; Laube and Veh, 1997) and monoclonal (Garthwaite et al, 1989; Schipper et al, 1991; Fujiwara et al, 1994; Delcros et al, 1995; Fujiwara and Masuyama, 1995) antibodies against the polyamines Put, Spd, and Spm have been produced and characterized. Generally, a considerable degree of cross‐reactivity was observed between anti‐Spd and anti‐Spm antibodies, whereas Put could be discriminated.…”

Section: Discussionmentioning

confidence: 99%

Differential distribution of spermidine/spermine‐like immunoreactivity in neurons of the adult rat brain

Laube

Bernstein

Wolf

et al. 2002

J of Comparative Neurology

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The polyamines spermidine and spermine are small, widely distributed polycations. In the brain, they confer rectification properties upon inwardly rectifying potassium channels and Ca(2+)-permeable alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionate (AMPA)/kainate receptors and also modify functional properties of N-methyl-D-aspartate receptors. Therefore, functional roles of spermidine/spermine in the adult brain will depend on the colocalization of the spermidine/spermine-sensitive receptors/channels and the polyamines either in the same or in closely associated cell types. We previously immunocytochemically demonstrated a prominent localization of spermidine/spermine in glial cells, especially astrocytes (Laube and Veh [ 1997] Glia 19:171-179). In contrast to the commonly accepted assumption of a ubiquitous distribution of polyamines in various cell types, in neurons of the rat brain, we detected a highly diverse spermidine/spermine-like immunoreactivity. The immunoreactivity in neurons and neuropil throughout the rat brain is listed according to intensity in arbitrary groups. The strongest neuronal staining was observed in the hypothalamic paraventricular, supraoptic, and accessory neurosecretory nuclei. Strong cytoplasmic staining was also evident in some motor and somatosensory areas such as the Me5 nucleus of the mesencephalic trigeminal tract, the nucleus ruber, and the large motor neurons of the spinal cord ventral horn. In contrast, in most cortical and hippocampal regions spermidine/spermine-like immunoreactivity in neurons was relatively weak, whereas in these areas, the labeling pattern was dominated by a diffuse neuropil labeling. In addition to spermidine/spermine immunocytochemistry, ornithine decarboxylase labeling was performed and the resulting labeling patterns were compared. The prominent localization of spermidine/spermine in neurosecretory neurons might point to a functional role different from channel/receptor modification. In these neurons, polyamines might be involved in secretory processes.

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“…There are several reports concerning the production and characterization of polyclonal (Bartos et al, 1975;Fujiwara et al, 1983;Quash and Wilson, 1967;Schipper et al, 1991) and monoclonal (Delcros et al, 1995;Fujiwara et al, 1994;Fujiwara and Masuyama, 1995;Garthwaite et al, 1989;Schipper et al, 1991) antibodies against the polyamines Put, Spd, and Spm. With polyclonal anti-Spd and anti-Spm antibodies, in most investigations and also in this study a considerable degree of cross-reactivity between Spd and Spm was observed.…”

mentioning

confidence: 97%