Preparation and in vitro evaluation of FGF-2 incorporated carboxymethyl chitosan nanoparticles

Nguyen, Cong-Thuan; Nguyen, Thanh-Thao; Nguyen, Thu-Thuy; Nguyen, Phuong Phong; Nguyen, Anh Dzung; Tran, Linh Thuoc; Tran‐Van, Hieu

doi:10.1016/j.carbpol.2017.05.080

Cited by 28 publications

(6 citation statements)

References 32 publications

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“…At present, CMCS drug delivery system has been widely used in the treatment of human diseases ( 18 , 43 ), but few studies pay attention to the effect of loading AMPs on preventing fish diseases and improving immune response. In this study, the effect of oral CMCS-20H on serum biochemical indexes reflecting innate immunity was firstly investigated.…”

Section: Discussionmentioning

confidence: 99%

Oral Administration of Nanopeptide CMCS-20H Conspicuously Boosts Immunity and Precautionary Effect Against Bacterial Infection in Fish

et al. 2022

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Massive mortalities caused by bacterial infections in intensive aquaculture result in serious economic losses. In this study, a novel antimicrobial peptide gcIFN-20H was efficiently expressed in Pichia pastoris (GS115) and loaded on carboxylmethyl chitosan (CMCS) to prepare CMCS-20H nanoparticles. Through physical characterization assays (TEM, DLS, BCA, and Raman) and biological activity tests (antimicrobial activity and cytotoxicity), CMCS-20H nanopeptide was verified to be spherical nanoparticles with sustained release, antimicrobial activity, and negligible toxicity. CMCS-20H nanoparticles are more resistant to intestinal degradation than unloaded gcIFN-20H by indirect immunofluorescence assay. Oral administration was then carried out for 42 days. Complement C3 content, lysozyme, and total superoxide dismutase activities are highest in CMCS-20H group by serum biochemistry index assays. After challenge with Aeromonas hydrophila, the survival rate in CMCS-20H group is highest (46%), which is 64% higher than the control group (28%). Meanwhile, the tissue bacterial loads (intestine, spleen, head kidney, trunk kidney, hepatopancreas, muscle, and blood) in the CMCS-20H group are significantly lower than other groups. By PAS staining analysis, the number of intestinal villi goblet cells and the thickness of mucin in the CMCS-20H group obviously increased. CMCS-20H effectively enhances mRNA expressions of some important immune genes (IL-1β, IL-6, TNF-α, IL-2, IFN-γ2, and IgM). The minimal tissue lesions (Intestine, spleen, and trunk kidney) were seen in the CMCS-20H group by histopathological examination. 16S rRNA sequencing showed that oral CMCS-20H maintains the intestinal microbiome homeostasis in bacterial infection. The results indicate that the novel nanopeptide CMCS-20H as the immunopotentiator can remarkably boost fish immunity and precautionary effect by oral administration and address the theoretical mechanisms and insights into the promising application prospect in aquaculture.

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Section: Discussionmentioning

confidence: 99%

Oral Administration of Nanopeptide CMCS-20H Conspicuously Boosts Immunity and Precautionary Effect Against Bacterial Infection in Fish

et al. 2022

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“…Nguyen et al incorporated fibroblast growth factor 2 (FGF-2), a protein that participates in wound healing but highly unstable in vivo, in carboxylmethyl chitosan (CMCS) nanoparticles [71]. Nanoparticles composed of CMCS:CaCl 2 (ratio of 1:0.8) with 95% of encapsulation efficiency for FGF-2 were produced using CaCl 2 as crosslinking agent.…”

Section: Polymeric Nanoparticlesmentioning

confidence: 99%

New Nanotechnologies for the Treatment and Repair of Skin Burns Infections

Souto

Ribeiro

Ferreira

et al. 2020

IJMS

102

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Burn wounds are highly debilitating injuries, with significant morbidity and mortality rates worldwide. In association with the damage of the skin integrity, the risk of infection is increased, posing an obstacle to healing and potentially leading to sepsis. Another limitation against healing is associated with antibiotic resistance mainly due to the use of systemic antibiotics for the treatment of localized infections. Nanotechnology has been successful in finding strategies to incorporate antibiotics in nanoparticles for the treatment of local wounds, thereby avoiding the systemic exposure to the drug. This review focuses on the most recent advances on the use of nanoparticles in wound dressing formulations and in tissue engineering for the treatment of burn wound infections.

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“…FGF also upregulates the expression of Akt, which activates eNOS, STAT3, PI3K, and MMP, responsible for tissue growth and angiogenesis [ 176 ]. Nguyen et al in 2017 prepared FGF-loaded carboxyl methyl chitosan (CMCS) nanoparticles (FGF-CMCS NPS) using an ionic gelation method for biological applications and FGF-2 remains protected from degradation of trypsin and thus act as an efficient way of FGF2 delivery at wound site for in vivo applications [ 177 ] (Fig. 8 L).…”

Section: Growth Factors and Growth Regulators In Healing Woundsmentioning

confidence: 99%

Protein-modified nanomaterials: emerging trends in skin wound healing

Sharda,

Kaur,

Choudhury

2023

Discover Nano

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Prolonged inflammation can impede wound healing, which is regulated by several proteins and cytokines, including IL-4, IL-10, IL-13, and TGF-β. Concentration-dependent effects of these molecules at the target site have been investigated by researchers to develop them as wound-healing agents by regulating signaling strength. Nanotechnology has provided a promising approach to achieve tissue-targeted delivery and increased effective concentration by developing protein-functionalized nanoparticles with growth factors (EGF, IGF, FGF, PDGF, TGF-β, TNF-α, and VEGF), antidiabetic wound-healing agents (insulin), and extracellular proteins (keratin, heparin, and silk fibroin). These molecules play critical roles in promoting cell proliferation, migration, ECM production, angiogenesis, and inflammation regulation. Therefore, protein-functionalized nanoparticles have emerged as a potential strategy for improving wound healing in delayed or impaired healing cases. This review summarizes the preparation and applications of these nanoparticles for normal or diabetic wound healing and highlights their potential to enhance wound healing. Graphical abstract

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Preparation and in vitro evaluation of FGF-2 incorporated carboxymethyl chitosan nanoparticles

Cited by 28 publications

References 32 publications

Oral Administration of Nanopeptide CMCS-20H Conspicuously Boosts Immunity and Precautionary Effect Against Bacterial Infection in Fish

Oral Administration of Nanopeptide CMCS-20H Conspicuously Boosts Immunity and Precautionary Effect Against Bacterial Infection in Fish

New Nanotechnologies for the Treatment and Repair of Skin Burns Infections

Protein-modified nanomaterials: emerging trends in skin wound healing

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