Preparation, Characterization and Anti-Complementary Activity of Three Novel Polysaccharides from Cordyceps militaris

Abstract

This investigation focuses on the three novel polysaccharides from Cordyceps militaris and then discusses their characterization and anti-complementary activity. The three polysaccharides from C. militaris (CMP-1, CMP-2 and CMP-3) were prepared using a DEAE-52 cellulose column. The HPLC, HPGPC, FT-IR and Congo red analyses were used to characterize their monosaccharides, molecular weight and stereo conformation, which demonstrated that the three polysaccharides were homogenous polysaccharides with different molecular weights and were composed of at least ten monosaccharides with different molar ratios, and all had a triple-helix conformation. The evaluation of anti-complementary activity demonstrated that the three polysaccharides significantly inhibited complement activation through the classical pathway and alternative pathway. Preliminary mechanism studies indicated that CMP-1, CMP-2 and CMP-3 acted with C2, C5, C9, factor B, factor B, and P components in the overactivation cascade of the complement system. The analysis of the Pearson correlation and network confirmed that the ribose, glucuronic acid and galacturonic acid composition were negatively correlated with the anti-complementary activity of polysaccharides. These results suggested that the three novel polysaccharides are potential candidates for anti-complementary drugs.

Polygonatum odoratum polysaccharide attenuates lipopolysaccharide‐induced lung injury in mice by regulating gut microbiota

“…The POP was prepared by water extraction and the alcohol precipitation method according to previous study (Fu et al, 2020 ; Hu et al, 2022 ). Briefly, the material of P. odoratum was crushed into powder and refluxed twice with deionized water (liquid–solid ratio at 10:1) at 100°C for 2 h. The extracted solution (about 5 L) was pooled and further concentrated to 1/7 of the initial volume under reduced pressure at 50°C.…”

A Systematic Review on the Research Progress on Polysaccharides from Fungal Traditional Chinese Medicine

“…The specific parameters are shown in Table 3. Hu et al [68] purified three new polysaccharides from Cordyceps militaris, and their total sugar contents were all higher than 90%. And their molecular weights were observed to be significantly higher than those of the polysaccharides that were reported previously.…”

“…Three polysaccharides were subjected to DEAE-52 column chromatography, collected, concentrated, dialyzed and lyophilized. Similarly, Hu et al [68] obtained three novel polysaccharides from Cordyceps militaris using this method. Sun et al [69] prepared a novel neutral exopolysaccharide (EPS-III) from culture broth of Cordyceps militaris.…”

Recent Advances in Polysaccharides from Chaenomeles speciosa (Sweet) Nakai.: Extraction, Purification, Structural Characteristics, Health Benefits, and Applications

“…Molecular weight (MW) is also considered to be a crucial factor in the structure characterization of C. speciosa polysaccharides. Common methods used to determine the molecular weights of polysaccharides include HPGPC, vapor osmotic pressure (VPO), viscosity, light scattering, mass spectrometry (MS), and chromatography [47,48]. Based on these techniques, the MWs of various C. speciosa polysaccharides mentioned and discussed in this paper are shown in Table 1.…”