Preparation, characterization, and anti-inflammatory effect of the chelate ofFlammulina velutipespolysaccharide with Zn

Abstract

The optimal conditions for preparing Zn-Flammulina velutipes polysaccharide (Zn-FVP) chelate were studied by using L 9 (3 4) orthogonal test. The results showed that the optimized conditions for the chelating reaction were as follows: initial concentration of Zn (II), 6 mg/mL; ratio of purified polysaccharide (FVPp) to Zn (II), 1:2; chelating time, 8 h; and pH, 5. Chelating rate achieved 98.11% under the optimal conditions. Characteristic bands for OH stretching at 3000-3500 cm −1 and bands corresponding to C-H variable angle vibration between 1000-1100 cm −1 showed some obvious differences for chelation of polysaccharides and zinc. The qualitative spectrum of Zn-FVP indicated the presence of ZnSO 4 and the abundant Zn (II) groups were widely present in the chelated samples. The anti-inflammatory effect of FVPp and Zn-FVP on Lipopolysaccharide-stimulated RAW 264.7 cells was evaluated. In the range of 5-50 μg/mL, Zn-FVP did not exhibit obvious inhibition of the survival rate of cells. Zn-FVP in noncytotoxic levels showed strong suppressive effect on the inflammatory cytokines (IL-6, TNF-α, INF-γ and NO) and these provided a theoretical base for the anti-inflammatory mechanism of Zn-FVP.

Therapeutic effects ofLigularia stenocephalaagainst inflammatory bowel disease by regulating antioxidant and inflammatory mediators

“…It is in good agreement with the previous comparative studies on antioxidant activity of plant extracts (Debnath et al, 2011;Samad et al, 2014). LPS stimulates macrophages to produce NO and inflammatory cytokines as well (Zhao, Li, Chen, Hu, & Zhao, 2017). The effect of LSW on the viability of RAW 264.7 cells was examined by the MTT method.…”

Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes

“…Zhao et al . found that the combination of Zn and purified polysaccharide could change the immunoactivity of this polysaccharide, and exhibited its anti-inflammatory effect through inhibition of inflammatory cytokines release including IL-6, TNF-α, INF-γ and NO 33 . In this study, we demonstrated that FVPB2 had a pronounced stimulatory effect on the activation and immunoglobulin production of B cells.…”

Inorganic or organic zinc and MUC-2, IgA, IL-17, TGF-β4 gene expression and sIgA secretion in broiler chickens

“…Follicular inductive sites in the intestine are rich in cytokines with IgA-inducing functions, including transforming growth factor β (TGF-β) and interleukin 17 (IL-17) (Cerutti & Rescigno, 2008). On the other hand, zinc is known to modulate some immunological parameters in vitro (Zhao, Li, Chen, Hu, & Zhao, 2016) and in broilers has a positive effect on the immunological capacity by improving the levels of immunoglobulins IgA, IgM, and IgG (Vieira et al, 2013). Zinc supplementation decreases oxidative stress markers and increases phagocytosis, Moreover, Zn promotes monocyte adhesion to endothelial cells in vitro and is important for the production of pro-inflammatory cytokines (Bonaventura, Benedetti, Albarède, & Miossec, 2015).…”