Preparation, characterization, and antioxidant activity of water-soluble oligochitosan

Nguyễn, Như Gia; Hoang, Dung Van; Nguyen, Ngoc Duy; Hiến, Nguyễn Quốc; Nguyen, Dai Hai

doi:10.1515/gps-2016-0126

Cited by 25 publications

(29 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…According to the previous studies, the degradation of chitosan in the heterogeneous state in the presence of hydrogen peroxide solution is quite efficient [9,20]. However, if the concentration of hydrogen peroxide was at the high level, the main chain structure of chitosan may be changed [20].…”

Section: Preparation Of Chitosan Of Different Molecular Weightsmentioning

confidence: 98%

“…is ATBS •+ solution was kept in the dark for 24 h. en, the ATBS •+ solution was diluted by water to receive an optical density (OD) of 1 ± 0.1 at a wavelength of 734 nm, after which the solution was also diluted with water for the control. For investigating antioxidant activity, the WSC samples with concentration of 0.2% were mixed with 0.1% acetic acid [9]. About 0.6 mL of solution sample was added into the cuvette that contained 1 mL of ABTS •+ solution.…”

Section: Antioxidant Assay Antioxidant Assay Was Carried Out By Dissmentioning

confidence: 99%

“…However, if the concentration of hydrogen peroxide was at the high level, the main chain structure of chitosan may be changed [20]. e way around this situation is the choice of 1% H 2 O 2 [9,13]. In addition, the degradation of chitosan in the heterogeneous state was almost supposed that it occurred in the amorphous region, and as a result, the reduction of molar mass of chitosan is only reached to a certain extent.…”

Section: Preparation Of Chitosan Of Different Molecular Weightsmentioning

confidence: 99%

“…is cause restricted applications of chitosan, and therefore, modification of chitosan with the aim of enhancing its solubility has attracted a great deal of research attention [3,6]. Recently, studies on chitosan modified with resultant products as natural antioxidants were carried out [2,[7][8][9]. However, these studies have only focused on preparation of low molecular weight chitosan or oligomers due to their easy solubility in water and high antioxidant activity [10].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, the antioxidant activity of high molecular weight chitosan [8] and chitosan derivatives [11] has also been investigated. Generally, the lower the molecular weight of the chitosan, the higher the solubility obtained [9]. is means that, chitosan with low molecular weight had a merit in applied ability due to soluble potency.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Study on Preparation of Water-Soluble Chitosan with Varying Molecular Weights and Its Antioxidant Activity

Dự¹,

Vương²

2019

Advances in Materials Science and Engineering

View full text Add to dashboard Cite

The preparation of water-soluble chitosan (WSC) with various molecular weights by gamma Co-60 irradiation of chitosan solution (5%) in the presence of hydrogen peroxide (1%) combined with acetylated reaction was carried out. The average molecular weight (Mw) of chitosan was measured by gel permeation chromatography (GPC). The chemical structure and the crystallinity of chitosan and WSC were characterized by Fourier-transform infrared (FT-IR) spectroscopy and X-ray diffraction (XRD), respectively. The antioxidant activity of WSC and chitosan was investigated using the free radical 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS•+). Obtained results showed that chitosan with Mw of 18–90 kDa could be efficiently prepared by this technique in the dose range from 10 to 24.5 kGy. After the acetylated process, the resulting WSC possesses good solubility in a wide pH level of 2–12, and WSC with low molecular weight exhibited significantly higher antioxidant activity than the one with high molecular weight. In detail, the antioxidant activity was 14.7%, 70.5%, 84.2%, 89.4%, and 97.5% for WSC samples prepared from chitosan with Mw of 140.2 kDa, 91.4 kDa, 51.2 kDa, 35.3 kDa, and 18.1 kDa, respectively, at the same reaction time of 90 min. Moreover, the antioxidant activity of WSC was higher than that of chitosan. Thus, WSC prepared by gamma Co-60 irradiation and acetylated process can be potentially applied as a natural antioxidant agent.

show abstract

Section: Preparation Of Chitosan Of Different Molecular Weightsmentioning

confidence: 98%

Section: Antioxidant Assay Antioxidant Assay Was Carried Out By Dissmentioning

confidence: 99%

Section: Preparation Of Chitosan Of Different Molecular Weightsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Study on Preparation of Water-Soluble Chitosan with Varying Molecular Weights and Its Antioxidant Activity

Dự¹,

Vương²

2019

Advances in Materials Science and Engineering

View full text Add to dashboard Cite

show abstract

A green approach for depolymerization of chitosan: applications in hydrogels

Tabassum,

Ahmed,

Ittisaf

et al. 2023

Cellulose

View full text Add to dashboard Cite

Chitooligosaccharides (COS) are depolymerized chains produced from the natural polymer chitosan and has been determined to exhibit improved biological activities, high solubility in neutral to slightly alkaline pH, because of the lower molecular weight (MW). This makes COS more attractive in biomedical applications. However, earlier studies focused on depolymerization techniques that were either cumbersome or expensive. Here, a convenient two-stage, green synthesis approach was developed and optimized, where gamma irradiation and oxidative degradation with H2O2 were used to depolymerize chitosan to produce COS for biomedical applications. The gamma radiation dose level, H2O2 degradation reaction’s temperature, time and H2O2 concentration were varied to obtain the mildest combination of reaction conditions. The most optimum set of conditions (15 kGy, 25oC, overnight reaction with 2% H2O2) yielded COS that was soluble in physiological pH range (7–8.5). The COS had a MW of 12.8 ± 1.6 kDa (which was a 95% reduction in MW), a 62.3% degree of deacetylation, and a crystallinity index of 33%. A photopolymerized hydrogel using this COS cross-linked with polyethylene glycol diacrylate (PEGDA) and carboxymethyl cellulose (CMC) was also developed. The hydrogel exhibited high swelling ratio (6.44–10.24), a porous morphology, a compression modulus of 4.5 ± 2.7 kPa (similar to soft tissues), and more than 95% biocompatibility with mammalian cells. This newly developed COS hydrogel involves a simple and green approach for the production of COS and shows promise as a scaffold for artificial soft tissue.

show abstract

Preparation of Chitooligosaccharide by Hydrogen Peroxide Degradation of Chitosan and Its Effect on Soybean Seed Germination

et al. 2019

View full text Add to dashboard Cite

Preparation, characterization, and antioxidant activity of water-soluble oligochitosan

Abstract: The preparation of oligochitosan through the heterogeneous degradation of chitosan/H

Cited by 25 publications

References 21 publications

Study on Preparation of Water-Soluble Chitosan with Varying Molecular Weights and Its Antioxidant Activity

Study on Preparation of Water-Soluble Chitosan with Varying Molecular Weights and Its Antioxidant Activity

A green approach for depolymerization of chitosan: applications in hydrogels

Preparation of Chitooligosaccharide by Hydrogen Peroxide Degradation of Chitosan and Its Effect on Soybean Seed Germination

Contact Info

Product

Resources

About