Preparation, characterization and in-vitro evaluation of sustained release protein-loaded nanoparticles based on biodegradable polymers

Abstract

Controlled drug delivery technology of proteins/peptides from biodegradable nanoparticles has emerged as one of the eminent areas to overcome formulation associated problems of the macromolecules. The purpose of the present investigation was to develop protein-loaded nanoparticles using biodegradable polymer poly L-lactide-co-glycolidic acid (PLGA) with bovine serum albumin (BSA) as a model protein. Despite many studies available with PLGA-based protein-loaded nanoparticles, production know-how, process parameters, protein loading, duration of protein release, narrowing polydispersity of particles have not been investigated enough to scale up manufacturing of protein-loaded nanoparticles in formulations. Different process parameters such as protein/polymer ratio, homogenizing speed during emulsifi cations, particle surface morphology and surface charges, particle size analysis and in-vitro protein release were investigated. The in-vitro protein release study suggests that release profi le of BSA from nanoparticles could be modulated by changing protein-polymer ratios and/or by varying homogenizing speed during multiple-emulsion preparation technique. The formulation prepared with protein-polymer ratio of 1:60 at 17,500 rpm gave maximum protein-loading, minimum polydispersion with maximally sustained protein release pattern, among the prepared formulations. Decreased (10,000 rpm) or enhanced (24,000 rpm) homogenizing speeds resulted in increased polydispersion with larger particles having no better protein-loading and -release profi les in the present study.

Preparation of bioactive interferon alpha– loaded polysaccharide nanoparticles using a new approach of temperature-induced water phase/water-phase emulsion

“…However, some also expose the hydrophobic cores, leading to protein instability (eg, poly(lactic-co-glycolic acid) [PLGA], PLGA-PEG nanoparticles), burst release, irregular nanospherical shapes or low encapsulating efficiency (eg, nanogel, liposomes, polymersomes), and incomplete release. [22][23][24][25][26][27][28][29][30][31][32][33] We have studied a solution that avoids vapor-water and water-oil interfaces for the preparation of bioactive protein microparticles, 19,[34][35][36][37] but the solution cannot be prepared for nanoscale particles, which has resulted in no further preparation of a targeteddelivery protein system.…”

Pressure-sensitive mucoadhesive polymer-based dental patches to treat periodontal diseases: anin vitrostudy

“…Investigation of interaction between drug and excipients is an important preformulation study which predominantly indicates the stability of the drug in the formulation and its release pattern (Mukherjee et al, 2008). Methods such as FTIR spectroscopy, IR spectroscopy, differential scanning calorimetry, etc.…”

Andrographolide nanoparticles in leishmaniasis: characterization and in vitro evaluations

“…The biopolymer blend did not show any absorbance in specific regions of AG reactive functional groups. 20 When AG was blended with PLGA and PVA and scanned in FTIR, all AG functional groups remained intact in position, showing that there was no appreciable chemical interaction between AG and the biopolymer materials used.…”