Preparation, characterization and stability assessment of keratin and albumin functionalized gold nanoparticles for biomedical applications

Thilagam, R.; Gnanamani, A.

doi:10.1007/s13204-020-01250-z

Cited by 30 publications

(9 citation statements)

References 76 publications

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“…In 2020, Gnanamani reported a study where keratin and albumin were used to improve the biocompatibility and storage stability of gold nanoparticles (AuNPs) [27]. The authors prepared blank AuNPs, keratin-functionalized AuNPs (KP-AuNPs), albuminfunctionalized AuNPs (AP-AuNPs), and keratin-albumin-functionalized AuNPs (KP-AP-AuNPs) by mixing an aqueous solution of 0.01 M HAuCl 4…”

Section: Human Hair Keratin-based Nanoparticlesmentioning

confidence: 99%

Keratin-Based Nanoparticles as Drug Delivery Carriers

Ferroni

Varchi

2021

Applied Sciences

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Keratin is a structural protein of mammalian tissues and birds, representing the principal constituent of hair, nails, skin, wool, hooves, horns, beaks, and feathers, and playing an essential role in protecting the body from external harassment. Due to its intrinsic features such as biocompatibility, biodegradability, responsiveness to specific biological environment, and physical–chemical properties, keratin has been extensively explored in the production of nanocarriers of active principles for different biomedical applications. In the present review paper, we aimed to give a literature overview of keratin-based nanoparticles produced starting from human hair, wool, and chicken feathers. Along with the chemical and structural description of keratin nanoparticles, selected in vitro and in vivo biological data are also discussed to provide a more comprehensive framework of possible fields of application of this protein. Despite the considerable number of papers describing the production and use of keratin nanoparticles as carries of anticancer and antimicrobial drugs or as hemostatic and wound healing materials, still, efforts are needed to implement keratin nanoparticles towards their clinical application.

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Section: Human Hair Keratin-based Nanoparticlesmentioning

confidence: 99%

Keratin-Based Nanoparticles as Drug Delivery Carriers

Ferroni

Varchi

2021

Applied Sciences

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“…140 With the deepening of research, people have discovered using specific proteins in the human body to decorated Au NPs can improve their long-term stability and avoid immune rejection. 141 This discovery is expected to be a drug delivery system that uses Au NPs as a carrier. On the other hand, some protein-decorated Au NPs exhibit characteristics that are not available in conventional synthetic Au NPs.…”

Section: Low Molecular Weight Protein Decorated Au Npsmentioning

confidence: 99%

High Stability Au NPs: From Design to Application in Nanomedicine

Zhang¹,

Shao²,

Yue³

et al. 2021

IJN

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In recent years, Au-based nanomaterials are widely used in nanomedicine and biosensors due to their excellent physical and chemical properties. However, these applications require Au NPs to have excellent stability in different environments, such as extreme pH, high temperature, high concentration ions, and various biomatrix. To meet the requirement of multiple applications, many synthetic substances and natural products are used to prepare highly stable Au NPs. Because of this, we aim at offering an update comprehensive summary of preparation high stability Au NPs. In addition, we discuss its application in nanomedicine. The contents of this review are based on a balanced combination of our studies and selected research studies done by worldwide academic groups. First, we address some critical methods for preparing highly stable Au NPs using polymers, including heterocyclic substances, polyethylene glycols, amines, and thiol, then pay attention to natural product progress Au NPs. Then, we sum up the stability of various Au NPs in different stored times, ions solution, pH, temperature, and biomatrix. Finally, the application of Au NPs in nanomedicine, such as drug delivery, bioimaging, photothermal therapy (PTT), clinical diagnosis, nanozyme, and radiotherapy (RT), was addressed concentratedly.

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“…The characteristic time of desorption of molecules of the "soft" corona is several hours, while the "hard" corona is stable for a long time [53,54]. Some conjugates of proteins with nanoparticles have been shown to retain activity for more than six months [55]. Thus, the stability of the conjugates directly depends on their composition.…”

Section: Calculation Of the Numbers Of Immunoglobulin G Sorbed On Gnpsmentioning

confidence: 99%

Retention of Activity by Antibodies Immobilized on Gold Nanoparticles of Different Sizes: Fluorometric Method of Determination and Comparative Evaluation

et al. 2021

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Antibody–nanoparticle conjugates are widely used analytical reagents. An informative parameter reflecting the conjugates’ properties is the number of antibodies per nanoparticle that retain their antigen-binding ability. Estimation of this parameter is characterized by a lack of simple, reproducible methods. The proposed method is based on the registration of fluorescence of tryptophan residues contained in proteins and combines sequential measurements of first the immobilized antibody number and then the bound protein antigen number. Requirements for the measurement procedure have been determined to ensure reliable and accurate results. Using the developed technique, preparations of spherical gold nanoparticles obtained by the most common method of citrate reduction of gold salts (the Turkevich–Frens method) and varying in average diameter from 15 to 55 nm have been characterized. It was shown that the number of antibodies (immunoglobulins G) bound by one nanoparticle ranged from 30 to 194 during adsorptive unoriented monolayer immobilization. C-reactive protein was considered as the model antigen. The percentage of antibody valences that retained their antigen-binding properties in the conjugate increased from 17 to 34% with an increase in the diameter of gold nanoparticles. The proposed method and the results of the study provide tools to assess the capabilities of the preparations of gold nanoparticles and their conjugates as well as the expediency of seeking the best techniques for various practical purposes.

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Preparation, characterization and stability assessment of keratin and albumin functionalized gold nanoparticles for biomedical applications

Cited by 30 publications

References 76 publications

Keratin-Based Nanoparticles as Drug Delivery Carriers

Keratin-Based Nanoparticles as Drug Delivery Carriers

High Stability Au NPs: From Design to Application in Nanomedicine

Retention of Activity by Antibodies Immobilized on Gold Nanoparticles of Different Sizes: Fluorometric Method of Determination and Comparative Evaluation

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